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Bromelain-coated fluorescent platinum nano-cluster as well as preparation method and application thereof

A bromelain and platinum nanotechnology, which is applied in the preparation and application of functional fluorescent nanomaterials, can solve the problems of long pretreatment time, complex requirements, and complicated processes, and achieve the effects of low detection cost, high sensitivity, and simple operation

Active Publication Date: 2019-08-02
ANHUI NORMAL UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, these methods not only require complex and precise instruments, but also require a long time for sample pretreatment, complicate the process, and consume financial resources.

Method used

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  • Bromelain-coated fluorescent platinum nano-cluster as well as preparation method and application thereof
  • Bromelain-coated fluorescent platinum nano-cluster as well as preparation method and application thereof
  • Bromelain-coated fluorescent platinum nano-cluster as well as preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0038] Embodiment 1: Preparation of Bromelain-PtNCs

[0039] Take 1.6mL bromelain (Bromelain) in an EP tube, then add 0.4mL 25mM chloroplatinic acid, the molar ratio of bromelain and chloroplatinic acid is 1:13, mix well with a vortexer for 5min; add 1M NaOH on top In the one-step solution, make the pH of the solution 12, mix again with a vortex for 5 minutes, and slowly add NaBH with a concentration of 0.5M 460 μL, the solution changed from light yellow to brown, and the EP tube was incubated in a 60°C water bath for 12 hours under the condition of avoiding light. The product was dialyzed in a 1000Da dialysis bag for 18 hours to obtain a polymer of platinum nanoclusters (Bromelain-PtNCs), which was then stored at 4°C in the dark.

Embodiment 2

[0041] Take 1.6mL bromelain in the EP tube, then add 0.4mL 25mM chloroplatinic acid, the molar ratio of bromelain and chloroplatinic acid is 1:10, mix well with a vortexer for 5min; add 1M NaOH to the solution in the previous step In the solution, the pH value of the solution was 12, and the vortex was mixed again for 5 minutes, and NaBH with a concentration of 0.5M was slowly added 4 60 μL, the solution changed from light yellow to brown, and the EP tube was incubated in a 60°C water bath for 12 hours under the condition of avoiding light. The product was dialyzed in a 1000Da dialysis bag for 18 hours to obtain a polymer of platinum nanoclusters, which was then stored at a low temperature at 4°C and protected from light.

Embodiment 3

[0043] Take 1.6mL bromelain in the EP tube, then add 0.4mL 25mM chloroplatinic acid, the molar ratio of bromelain and chloroplatinic acid is 1:15, mix well with a vortexer for 5min; add 1M NaOH to the solution in the previous step In the solution, the pH value of the solution was 12, and the vortex was mixed again for 5 minutes, and NaBH with a concentration of 0.5M was slowly added 4 60 μL, the solution changed from light yellow to brown, and the EP tube was incubated in a 60°C water bath for 12 hours under the condition of avoiding light. The product was dialyzed in a 1000Da dialysis bag for 18 hours to obtain a polymer of platinum nanoclusters, which was then stored at a low temperature at 4°C and protected from light.

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Abstract

The invention belongs to the field of preparation and application of functional nano fluorescent materials, and discloses a bromelain-coated fluorescent platinum nanocluster and a preparation method and application thereof. The novel bromelain-coated fluorescent platinum nanocluster is prepared by an environment-friendly one-step synthesis method, and the method comprises the following steps: mixing bromelain and chloroplatinic acid, adjusting the pH value, slowly adding NaBH4 until the solution turns from faint yellow to brown, then carrying out water bath, and dialyzing to obtain the bromelain-coated fluorescent platinum nanocluster. The material can be used for detecting the Fe < 3 + > content in a water sample, and the detection method is simple, convenient, efficient, high in specificity and high in sensitivity and has an important value for detection of an actual sample containing heavy metal ions. The bromelain-coated fluorescent platinum nanocluster can also be used for tracking the distribution and effect of the bromelain-coated fluorescent platinum nanocluster in the application fields of foods, cosmetics, medicines and treatment.

Description

technical field [0001] The invention belongs to the field of preparation and application of functional fluorescent nanometer materials, in particular to a fluorescent platinum nanocluster wrapped by bromelain, a preparation method and application thereof. Background technique [0002] Platinum nanocluster is a new type of nanomaterial composed of several to tens of platinum atoms, and its size generally does not exceed 2nm. Platinum nanoclusters have many advantages such as good fluorescence properties, photostability, low toxicity, biocompatibility, and easy surface modification, and have attracted more and more attention from researchers. Due to the excellent characteristics of nanoclusters, it has a wide range of applications in heavy metal detection, biological imaging, etc., but it is difficult to synthesize platinum nanoclusters with high fluorescence quantum yield, good biocompatibility, and simple and easy repeatability. [0003] Excessive iron content in water can ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N21/64C12N11/14
CPCG01N21/6428C12N11/14C12N9/63C12Y304/22004G01N2021/6432
Inventor 李全发徐南南王源秀施雪琴王宝娟高鹏吴爽常欣熊声麒杨刚
Owner ANHUI NORMAL UNIV
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