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Application of total alkaloids of melodinuskhasianus Hook. f.

A technology of total alkaloids and drugs, applied in the field of medicine, can solve problems such as no patent literature reports, and achieve the effects of good development and application prospects, expanding value, and low price

Active Publication Date: 2019-08-02
云南聚和生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The whole plant of Jingdongshan orange can be used as a medicinal material, which has the effects of eliminating internal heat, antispasmodic, dissipating stasis, etc. It is often used for bruises and injuries; Jingdongshan orange is rich in monoterpene indole alkaloids Components such as terpenoids, sterols, and volatile oils have a good regulatory effect on the physiological functions of the body; at present, there are no patent literature reports on the research on the related diseases caused by the prevention and treatment of inflammation caused by the total alkaloids of Jingdongshan orange at home and abroad.

Method used

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  • Application of total alkaloids of melodinuskhasianus Hook. f.
  • Application of total alkaloids of melodinuskhasianus Hook. f.
  • Application of total alkaloids of melodinuskhasianus Hook. f.

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0023] Embodiment 1: Preparation of total alkaloids of Jingdongshan orange

[0024] The aboveground part of Jingdongshan orange was collected from Bijie, Guizhou, dried naturally and powdered to obtain the Jingdongshan orange sample; 100g of Jingdongshan orange sample was weighed, and the ratio of solid to liquid was g:mL=1:10. % methanol aqueous solution for 30 minutes, filtered, and the filter residue was repeated ultrasonic extraction for 3 times, the filtrate was combined, evaporated and concentrated at 45°C to obtain the extract; the extract was dissolved in 0.3% hydrochloric acid aqueous solution, and the pH was adjusted to 2-3 , extracted with ethyl acetate, collected the water layer, then added ammonia water with a volume concentration of 5%, adjusted the pH to 9-10, then extracted with ethyl acetate, collected the ethyl acetate layer, evaporated and concentrated, and freeze-dried to obtain the scene Dongshan orange total alkaloids (TAMK).

Embodiment 2

[0025] Example 2: Evaluation of anti-inflammatory activity at the cell level of RAW 264.7

[0026] LPS-induced RAW 264.7 was used to establish a cell inflammation model, and the expression of the total alkaloids of Jingdongshan orange on the expression of NO and related inflammatory factors in RAW264.7 cells was determined to evaluate the anti-inflammatory activity of the total alkaloids of Jingdongshan orange;

[0027] 1. Cell culture and non-toxic dosage screening

[0028] (1) Inoculate RAW 264.7 cells in DMEM containing 10% fetal bovine serum (FBS), 1% penicillin (100 U / mL) and streptomycin (100 mg / mL) mixed antibiotics, at 37°C, 5% Cultivate in a humid environment of CO2 and 95% air;

[0029] (2) The toxicity of the total alkaloids of Jingdongshan orange to RAW264.7 cells was determined by MTT method, and the cultured RAW264.7 cells were treated with 1×10 4 Each well was connected to a 96-well plate. After 24 hours of culture, the medium was sucked away. The sample group...

Embodiment 3

[0039] Example 3: Establishing a mouse ear swelling model to evaluate the anti-inflammatory activity of total alkaloids in Jingdongshan orange

[0040] The mouse ear swelling model was established by smearing xylene, and the anti-inflammatory activity of the total alkaloids of Jingdongshan orange was evaluated by measuring the weight change of the mouse ear before and after smearing;

[0041] 1. Establishment of mouse ear swelling model and activity evaluation

[0042] Male Kunming mice are 4-5 weeks old Kunming mice (18-22g), 8 mice in each group, and the experimental groups are as follows:

[0043] Group K: control group (distilled water gavage, K), Group M: model group (distilled water gavage, M), Group DEX: positive control group (dexamethasone 5mg / kg), Group TAMKL: total alkaloids of Jingdongshan orange Low-dose group 50mg / kg, GroupTAMKM: Jingdongshan orange total alkaloids middle-dose group 100mg / kg. Group TAMKH: Jingdongshan orange total alkaloid high dose group 200 m...

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Abstract

The invention discloses application of total alkaloids of melodinuskhasianus Hook. f., namely, the application of the total alkaloids of the melodinuskhasianus Hook. f. in preparation of drugs for preventing and / or treating inflammatory diseases, and belongs to the field of natural drug development or medical technologies; the total alkaloids part of the melodinuskhasianus Hook. f. is obtained bytaking the overground part of the melodinuskhasianus Hook. f. as a raw material, performing ultrasonic extraction with a methanol solution, and performing acid and alkali treatment; anti-inflammatoryactivity evaluation is performed on the total alkaloids of the melodinuskhasianus Hook. f. through an LPS (Lipopolysaccharide) induced RAW 264.7 cellular inflammatory model, and in-vivo anti-inflammatory activity verification is performed through mouse ear-swelling and claw-swelling models; experimental results verify that the total alkaloids of the melodinuskhasianus Hook. f. has certain improving effect on inflammation, can have an anti-inflammatory effect in vivo, and can prevent and treat incidence of inflammation related diseases; melodinuskhasianus Hook. f. plant raw materials are easy to obtain, and low in price; an alkaloids extraction process is simple; the total alkaloids of the melodinuskhasianus Hook. f. has good development and application prospect in preparation of the drugsfor preventing and treating the inflammatory diseases.

Description

technical field [0001] The invention relates to the application of total alkaloids of Jingdongshan orange in the preparation of medicines for preventing and treating inflammation and related diseases caused by inflammation, and belongs to the technical field of medicine. Background technique [0002] Inflammatory response is a common clinical pathological process, which is a protective immune response triggered by harmful stimuli and specific conditions such as infection, toxin and tissue damage. However, chronic inflammation is closely related to the pathogenesis of diseases including atherosclerosis, obesity, asthma, arthritis, cancer and autoimmune diseases. [0003] Epidemiological evidence shows that more than 25% of cancers are caused by chronic infection or other types of inflammation, many factors such as microbial infections, autoimmune diseases, and other inflammatory diseases (such as prostate cancer-related prostatitis) Promotes the transition from inflammation ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K36/24A61P29/00
CPCA61K36/24A61P29/00
Inventor 程桂广杨美莲何舒悦曹建新赵天瑞
Owner 云南聚和生物科技有限公司
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