A kind of stage-by-stage culture method of zebrafish

Abstract

The invention relates to a sectional zebra fish breeding method and belongs to zebra fish breeding methods. The method comprises a female fish breeding stage, a parent fish propagation stage, an embryo incubation stage, a larva fish breeding stage, an advanced fry breeding stage, a juvenile fish breeding stage and a grown fish breeding stage, wherein the light intensity of the female fish breedingstage, the parent fish propagation stage, the embryo incubation stage, the larva fish breeding stage, the advanced fry breeding stage, the juvenile fish breeding stage and the grown fish breeding stag is all 1000-1500 lux; the light darkness ratio is a lighting time; the dark time is 16 h:8 h, 14 h:10 h or 12 h:12 h; the water hardness is 130-150 mg*L<-1>; the pH value is 7.5-7.9. In addition, onthe premise that the breeding method provided by the invention does not use artificial synthesis substance which can effect growth and development of zebra fish, or antibiotics which can interfere test results and resistance of the zebra fish, a great amount of test fishes meeting experiment requirements can be bred, and the bred fishes are high in survival rate and low in death rate.

Description

technical field [0001] The invention belongs to the field of cultivating zebrafish, and in particular relates to a stage-by-stage culturing method of zebrafish. Background technique [0002] Water quality benchmarks are an indispensable scientific basis and theoretical basis for ecological environment testing departments to formulate water quality standards, evaluate water quality and conduct water quality management. Water quality standards for aquatic organisms include acute aquatic organisms water quality standards and chronic aquatic organisms water quality standards, which are an important basis for formulating water quality standards and acute aquatic ecological risk assessment. The Aquatic Biological Benchmark takes the aquatic organisms inhabiting the aquatic ecosystem as the protection target, and aims to maintain the integrity of the structure and function of the aquatic ecosystem and biodiversity. [0003] Zebrafish has the advantages of short growth cycle, stron...

AI Technical Summary

Problems solved by technology

[0004] The Chinese invention patent (CN201410158549.3 A high-density fish farming method) relates to a high-density fish farming method, including the following steps: 1) feeding fry, 2) fish pond sealing, 3) water flow regulation, 4) feeding feed, 5) drainage and sewage discharge, 6) fishing operation, although it can solve the problems of difficulty in further increasing the breeding density, complicated aeration equipment, high energy consumption, low utilization rate of pure oxygen, and inconvenient farming operation in the current factory fish farming, but this invention does not The growth process of fish is divided into different stages, and the division of different stages makes the growth rate of fish fast and the survival rate high, and it can cultivate fish in large quantities to meet the needs