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Fluorescent immunoassay set for simultaneous detection of diacetylmorphine, methamphetamine, ketamine and cocaine and its preparation and use method

A technology of diacetylmorphine and methamphetamine, applied in the field of biomedicine, can solve problems such as long consumption time, inability to meet on-site detection, easy addiction and the like

Active Publication Date: 2022-03-01
石家庄洹众生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

It is very easy to become addicted after a large amount of smoking, and the relapse rate exceeds 90%, it is difficult to quit
[0007] At present, the detection of these four drugs mainly uses infrared spectroscopy (FTIR), gas chromatography (GC), liquid chromatography (HPLC), capillary electrophoresis, gas chromatography-mass spectrometry (GC-MS) and liquid chromatography. Chromatography-mass spectrometry (HPLC-MS), etc., although these methods are relatively accurate in detection, they take a long time to detect, and have high requirements for experimenters and equipment, and cannot satisfy law enforcement personnel outside. on-site testing

Method used

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  • Fluorescent immunoassay set for simultaneous detection of diacetylmorphine, methamphetamine, ketamine and cocaine and its preparation and use method
  • Fluorescent immunoassay set for simultaneous detection of diacetylmorphine, methamphetamine, ketamine and cocaine and its preparation and use method
  • Fluorescent immunoassay set for simultaneous detection of diacetylmorphine, methamphetamine, ketamine and cocaine and its preparation and use method

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preparation example Construction

[0063] The present invention also provides the preparation method of fluorescent immunity test paper group described in above-mentioned technical scheme, comprises the following steps:

[0064] S1. Spray independent fluorescent microsphere-labeled diacetylmorphine monoclonal antibody region, fluorescent microsphere-labeled methamphetamine monoclonal antibody region, fluorescent microsphere-labeled ketamine monoclonal antibody region and fluorescent microsphere-labeled monoclonal antibody region to the binding pad. The cocaine monoclonal antibody region obtains the binding pad of the qualitative test paper; the fluorescent microsphere-labeled polyclonal antibody of diacetylmorphine, methamphetamine, ketamine and cocaine is sprayed on the binding pad to obtain the binding pad of the quantitative test paper;

[0065]S2, spray the mixture of diacetylmorphine, methamphetamine, ketamine and cocaine to NC film as detection line, spray IgG polyclonal antibody as quality control line, o...

Embodiment 1

[0079] The test paper set for simultaneously detecting four kinds of drugs of diacetylmorphine, methamphetamine, ketamine and cocaine according to the present invention comprises qualitative test paper and quantitative test paper. The structure of qualitative test paper and quantitative test paper is sample pad, binding pad, NC membrane and absorbent paper from bottom to top. The sample pad, conjugate pad, NC membrane and absorbent paper are all adhered to the PVC bottom plate. Both the sample pad and the binding pad are made of glass fiber membrane, the reaction membrane is nitrocellulose membrane, and the absorbent paper is strong absorbent paper. The specific structure is as figure 1 shown.

[0080] 1. Preparation of qualitative test paper:

[0081] The sample pad of the qualitative test strip is divided into four independent areas, which are the diacetylmorphine monoclonal antibody area labeled with fluorescent microspheres, the methamphetamine monoclonal antibody area ...

Embodiment 2

[0106] The establishment of embodiment 2 standard curve

[0107] Make a standard curve between the concentration of the standard substance and the final detection fluorescence intensity, so that quantitative analysis can be carried out through the fluorescence intensity of the sample.

[0108] Diacetylmorphine, methamphetamine, ketamine and cocaine of known concentrations were diluted to the following concentration series: 1000ng / mL, 500ng / mL, 250ng / mL, 100ng / mL, 50ng / mL, 25ng / mL, 10ng / mL , 0ng / mL.

[0109]Take 100 μL of samples of each concentration and drop them on the sample pad of the quantitative test paper constructed in Example 1, and let it stand at 25°C for 5 minutes; use an immunofluorescence analyzer to measure the fluorescence intensity of the test line and quality control line in the test paper after the reaction is completed. detection. The measurement was repeated three times, and the obtained fluorescence intensity signal T / C values ​​were taken as the ordina...

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Abstract

The invention provides a fluorescent immune test paper set for synchronous detection of diacetylmorphine, methamphetamine, ketamine and cocaine and its preparation and use method, which relate to the technical field of biomedicine. The fluorescent immune test paper set of the present invention includes qualitative test paper and quantitative test paper. Test paper; the sample pads of the qualitative test paper are respectively provided with independent four-drug fluorescent microsphere-labeled monoclonal antibody areas, and the detection line is sprayed with a mixture of the four drugs; the sample pads of the quantitative test paper are provided with the multi- Antibodies were cloned, and four drug antigens were sprayed on the NC membrane as four quantitative detection lines. The fluorescent immunological test paper set provided by the invention can simultaneously measure four kinds of drugs, and can realize qualitative and quantitative detection at one time after combining qualitative test paper and quantitative test paper, which saves time and effort, and is of great significance to the on-site detection of drug law enforcement.

Description

technical field [0001] The invention relates to the technical field of biomedicine, in particular to a fluorescent immune test paper set for synchronously detecting diacetylmorphine, methamphetamine, ketamine and cocaine and a preparation and use method thereof. Background technique [0002] Drug abuse has become a worldwide concern in recent years. Drugs are not only addictive, but also endanger people's physical and mental health, and have an irreparable impact on social tranquility. Therefore, we must stop this illegal behavior as quickly as possible, and rescue drug addicts from the environment of drug abuse in time to create a better society. Therefore, if law enforcement officers can quickly detect drugs on the spot, they can save drug users more effectively. [0003] Heroin is a kind of morphine drug, which is what we usually call opium. It is a semi-synthetic drug with strong drug dependence. Heroin can not only cause great harm to the human body, but also has a ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N33/533G01N33/543
CPCG01N33/533G01N33/54313
Inventor 李晓春李森
Owner 石家庄洹众生物科技有限公司
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