Response-surface-optimized coix seed polyphenol extracting method

Abstract

The invention discloses a response surface optimized coix seed polyphenol extraction method, and belongs to the technical field of coix seed polyphenol extraction. According to the response surface optimized coix seed polyphenol extraction method, an ultrasonic method is adopted to extract coix seed polyphenol, gallic acid is taken as a standard substance, foline-phenol is taken as a color developing agent, and the content of polyphenol is tested by a colorimetric method. The effects of the seven factors of a solvent type, a solid-liquid ratio, a solvent volume fraction, extraction temperature, extraction time, extraction times and particle size of a sample on the extraction rate of the coix seed polyphenol are investigated by an experiment. On the basis of a single factor experiment, a Box-Behnken response surface analysis method is adopted to design an experiment scheme, the solid-liquid ratio, the solvent volume fraction, the extraction time and the extraction temperature are takenas experimental factors, the extraction rate of the coix seed polyphenol is a response value, a regression model is established, the validity of the regression model and the influence degree of various single factors are analyzed, various factors during the coix seed polyphenols extraction are optimized, and optimized process conditions of the coix seed polyphenol extraction are acquired. A good fitting degree exists between an actually tested value and a theoretical value so that the method is suitable for the coix seed polyphenols extraction.

Description

technical field [0001] The invention belongs to the technical field of barley polyphenol extraction, and in particular relates to a response surface optimization method for extracting barley polyphenols. Background technique [0002] Barley, also known as coix seed, is the seed of coix after shelling. Barley is rich in nutrients, containing about 65% carbohydrates, 17% protein, and 5% fat; in addition to the three major energy substances, barley also contains a variety of Active ingredients, such as barley, vitamins, trace elements, polyphenols, etc., among which the content of polyphenols is higher than that of similar plants. Polyphenols are a class of secondary metabolites with a polyphenolic structure that widely exist in plants and are natural antioxidants. The human body cannot produce polyphenols by itself. Regular intake of polyphenol-rich foods is very necessary, which can effectively reduce the occurrence of cardiovascular diseases. Regular consumption of barley i...

AI Technical Summary

Problems solved by technology

[0006] The technical problem to be solved by the present invention is to provide a response surface optimization method for extracting polyphenols from barley to solve the technical problem that has not been reported in the prior art on the combination of ultrasound and response surface method to extract polyphenols from barley

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