Process method for extracting neophytadiene, carotenoid and nicotine from waste tobacco leaves

Abstract

The invention discloses a process method for extracting neophytadiene, carotenoid and nicotine from waste tobacco leaves, and the process method comprises the following steps of: crushing the waste tobacco leaves, adding an ethanol solution, performing twice extraction, combining two extraction solutions, concentrating the two extraction solutions and recovering ethanol to obtain a concentrated solution; adding the concentrated solution into an equal volume of an organic solvent for extraction; concentrating an extractive solution to 1 / 50 of the original volume, volatilizing the solvent to dry, and performing sample loading on a normal phase silica gel column; performing gradient elution with an organic solvent to collect eluents with different gradients, concentrating the eluents, and sequentially obtaining crude neophytadiene, crude carotenoid and crude nicotine products; dissolving the obtained crude neophytadiene, crude carotenoid and crude nicotine products with organic solvents respectively, performing sample loading on gel columns, eluting with organic solvents, respectively collecting eluates and concentrating the eluates to obtain the neophytadiene, the carotenoid and thenicotine. The three products of the neophytadiene, the carotenoid and the nicotine are synchronously separated by the method, and the process method has the advantages of no temperature control, simplicity and high yield, and is suitable for industrial production.

Description

technical field [0001] The invention relates to the technical field of tobacco product processing, in particular to a process for extracting neophytadiene, carotenoids and nicotine from waste tobacco leaves. Background technique [0002] my country is a big country in tobacco production and consumption. During tobacco planting and processing, a large amount of tobacco waste such as low-grade tobacco leaves, tobacco stems and tobacco dust is produced, accounting for 10% to 25% of the total output of tobacco leaves. At present, there are very few ways to comprehensively utilize these wastes, and the degree of comprehensive utilization is very low. Most of them are discarded as waste, causing great pollution to the environment. However, these discarded tobacco leaves are potentially valuable resources. More than 300 compounds have been identified from tobacco leaves, which contain not only important aroma-inducing substances of tobacco leaves such as neophytadiene and nicotine,...

AI Technical Summary

Problems solved by technology

[0003] As for the method of extracting waste tobacco leaves, there are disadvantages such as complex process and difficulty in industrial production, and the same method cannot be used to obtain various substances in waste tobacco leaves, such as described in patent documents CN109134179A and CN109134180A Two kinds of methods for industrialized production of neophytadiene have been proposed, but the purity of both obtained neophytadiene is not high, only reaching 50%, and wherein the patent No. is that the neophytadiene obtained in the technology of CN106501420B has higher purity, reaching 99.4%, but its process is complex, the cost is high, only limited to the use of laboratory preparation as a standard product, and cannot be industrially scaled up, and these two methods can only obtain neophytadiene, while carotenoids in waste tobacco leaves It is not only the main coloring substance in tobacco leaves, but also its degradation product is also an important aroma-generating substance in tobacco leaves. The extraction of this substance is also of great significance. Therefore, there is an urgent need for a process that can extract more than one component in waste tobacco leaves method, especially to extract substances such as neophytadiene and carotenoids

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