Rapid identification method of lipstatin metabolites in streptomyces toxytricini fermentation products

Abstract

The invention provides a rapid identification method of lipstatin metabolites in streptomyces toxytricini fermentation products, and belongs to the technical field of microbial fermentation product analysis. The invention aims to provide the practical, rapid and accurate rapid identification method of the lipstatin metabolites in the streptomyces toxytricini fermentation products. By analyzing profiles of Lipstatin compound fragment ions in streptomyces toxytricini fermentation liquor, comparing the Lipstatin compound fragment ions with characteristic fragment ions, and comprehensively parsinga mass fragmentation pattern of similar compounds, unknown Lipstatin compounds in the fermentation liquor can be subjected to rapid structure identification. The rapid identification method of the lipstatin metabolites in the streptomyces toxytricini fermentation products provided by the invention has simple operation, high sensitivity, strong anti-interference performance and good popularizationand application value.

Description

technical field [0001] The invention provides a rapid identification method for lipstatin metabolites, which are fermentation products of Streptomyces toxin, and belongs to the technical field of analysis of microbial fermentation products. Background technique [0002] Obesity has become a common disease worldwide and is a risk factor for many diseases (such as: non-insulin-dependent diabetes, heart disease, cerebrovascular disease, respiratory system disease, certain cancers), so research and development of safe and effective Weight loss drugs have become a research area of ​​concern in the world. [0003] Most of the weight-loss drugs that have been developed and applied at present act on the central nervous system, and are basically banned due to strong side effects. Pancrelipase inhibitor is the only weight-loss drug that does not act through the central nervous system so far. It selectively inhibits pancreatic lipase in the gastrointestinal tract to reduce the decompo...

AI Technical Summary

Problems solved by technology

Efficient chemical structure identification and analysis methods are crucial to the research in the field of biopharmaceuticals. The traditional analysis mode of separating and purifying natural products and then identifying their structures using spectral techniques is cumbersome, and it is difficult to repeatedly separate known components and obtain pure new components. And other issues

However, only high-performance liquid chromatography (HPLC) is used for component analysis. It takes a long time if the component separation is required to be good, and there is a problem that no UV-absorbing components cannot be detected.

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