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Preparation of acetylcholinesterase biosensor and application to organic phosphorus detection

An acetylcholinesterase and biosensor technology, applied in the acetylcholinesterase organophosphorus pesticide biosensor and its preparation, in the field of biosensors, can solve the problems of being unsuitable for on-site detection, expensive equipment, long measurement time, etc., and achieve a good biological phase. Capacitance and stability, increase electrode stability, resolve time-consuming effects

Active Publication Date: 2019-07-19
GUIZHOU UNIV
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, these methods are expensive in equipment and take a long time to measure, so they are not suitable for on-site detection.

Method used

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  • Preparation of acetylcholinesterase biosensor and application to organic phosphorus detection
  • Preparation of acetylcholinesterase biosensor and application to organic phosphorus detection
  • Preparation of acetylcholinesterase biosensor and application to organic phosphorus detection

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Embodiment Construction

[0014] In order to make the technical solutions and advantages of the present invention easier to be understood by those skilled in the art, the specific implementation of this case will be described in detail below in conjunction with the accompanying drawings. Obviously, the described implementation cases are only a part of the implementation cases of the present invention rather than all the implementation cases. Based on the implementation of the present invention, all other embodiments obtained by those of ordinary skill in the art without creative work are all Belong to the protection scope of the present invention.

[0015] The electrochemical sensor for detecting organic phosphorus in the invention uses a glassy carbon electrode as a working electrode, a silver chloride reference electrode, and a platinum wire electrode as a counter electrode.

[0016] 2 Experimental methods

[0017] 2.1 Preparation of copper nanowires by chemical reduction method, the specific steps ...

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Abstract

Copper nanowires are synthesized and are mixed with rGO-TEPA; a mixture is modified onto a glassy carbon electrode; after the drying at room temperature, a mixture of acetylcholinesterase and chitosanglutaraldehyde is modified onto the electrode; the electrode is put into a refrigerator for incubation to a dry state; and an AChE-CS / rGO-TEPA-Cu NWs / GCE (acetylcholinesterase-chitosan-glutaraldehyde / reductive graphene oxide-tetraethylenepentamine-copper nanowires / glassy carbon electrode) biosensor is built. An electrochemical biosensor used for organic phosphorus detection is built by using theinhibition effect of the organic phosphorus on acetylcholinesterase. The advantages of simplicity, convenience, high sensitivity and the like are realized. A novel space is developed for an organic phosphorus detection technology.

Description

technical field [0001] The invention belongs to the field of organophosphorus pesticides, and relates to biosensors, in particular to an acetylcholinesterase organophosphorus pesticide biosensor and a preparation method thereof. Background technique [0002] my country is a large agricultural country. The wide application of organophosphorus pesticides has played a huge role in preventing crop diseases and insect pests. However, with the long-term abuse of organophosphorus pesticides, it has become a major safety hazard that endangers environmental safety and human health. Therefore, it is particularly important to establish a safe and sensitive method for the detection of organophosphate. [0003] Traditional organophosphorus detection methods include: gas chromatography, liquid chromatography, enzyme-linked immunoassay and mass spectrometry. However, these methods are expensive in equipment and take a long time to measure, so they are not suitable for on-site detection. ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N27/30G01N27/327G01N27/48
CPCG01N27/308G01N27/3277G01N27/3278G01N27/48
Inventor 李升曲立梅
Owner GUIZHOU UNIV
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