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Drosophila culture medium and preparation and subpackaging method thereof

A preparation method and culture medium technology, applied in the directions of packaging, transportation and packaging, special packaging objects, etc., can solve the problems of affecting the growth and egg laying of fruit flies, poor antibacterial properties and culture effect, and high cost of fruit fly culture medium, reaching Improves the ability to resist the growth of microorganisms such as bacteria, is beneficial to sterilization, and has the effect of simple process

Inactive Publication Date: 2019-07-19
ANHUI NORMAL UNIV
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  • Abstract
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  • Application Information

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Problems solved by technology

[0004] The applicant found that the formula components and preparation methods of the above-mentioned medium need to be further optimized
Secondly, the high water content (or hardness) of the medium is easy to stick to the fruit flies, and the low water content will affect the growth and oviposition of the fruit flies.
In addition, the existing Drosophila culture medium has high cost, poor antibacterial performance and poor culture effect, and the packaging is done manually, which is cumbersome, time-consuming and uneven

Method used

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  • Drosophila culture medium and preparation and subpackaging method thereof
  • Drosophila culture medium and preparation and subpackaging method thereof

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Embodiment Construction

[0028] For the following, through the description of the examples, the specific implementation of the present invention, such as the manufacturing process involved, the operation and use method, etc., will be described in further detail, so as to help those skilled in the art to have a more complete concept of the invention and the technical solution of the present invention. , accurate and in-depth understanding.

[0029] Raw materials, reagents and equipment involved:

[0030] 1) Corn flour (Yuyuan brand): fine grade, Shandong Tengzhou Xinglong Food Co., Ltd.;

[0031] 2) Sucrose (A100355): Sangon Bioengineering (Shanghai) Co., Ltd.;

[0032] 3) Yeast powder (Anqi brand): Angel Yeast Co., Ltd.;

[0033] 4) Agar powder (A100637): Sangon Bioengineering (Shanghai) Co., Ltd.;

[0034] 5) Propionic acid (A601757): Sangon Bioengineering (Shanghai) Co., Ltd.;

[0035] 6) Methyl p-hydroxybenzoate (A601186): Sangon Bioengineering (Shanghai) Co., Ltd.;

[0036] 7) 95% ethanol (A5...

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Abstract

The invention discloses a drosophila culture medium and a preparation and subpackaging method thereof, and relates to the technical field of drosophila culture media. The proportion of various nutritional ingredients in a drosophila culture medium formula is optimized. Meanwhile, a methylparaben preservative is added to the culture medium, the preservative capacity is enhanced, and the agar content in the drosophila culture medium is optimized, and the hardness is optimized to make the drosophila culture medium suitable for growth of drosophila. Penicillin and streptomycin are added to effectively inhibit the growth of bacterial microorganisms; the antibacterial property of the culture medium is improved, and the performance test data show that each pair of drosophila of the drosophila culture medium prepared by the invention gives birth to 74.6 offspring, namely 74.6 offspring per pair which is much higher than the existing 43.4 offspring per pair.

Description

technical field [0001] The invention relates to the technical field of fruit fly culture medium, in particular to a fruit fly culture medium and a preparation and packaging method thereof. Background technique [0002] Drosophila (Fruit fly) is a small insect of the order Diptera, which is a complete metamorphosis development type, and its life cycle includes four developmental stages: egg, larva, pupa and adult. Drosophila has the characteristics of easy breeding, short life cycle, high reproductive rate, many mutation traits and easy observation. It is an excellent model organism for studying the regulation mechanism of life activities and is widely used in genetics, neurobiology and developmental biology. Many fields of research in the life sciences such as science. During the research process, raising fruit flies with high quality is the basic guarantee for the normal operation of the experiment. Drosophila has many advantages as experimental materials: first, the prep...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01K67/033B65B3/12
CPCA01K67/033B65B3/12
Inventor 陈冬生孙福玲孙铭钟窦运乔吴华勤
Owner ANHUI NORMAL UNIV
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