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A Portable AHL Molecularly Imprinted Screen Printed Electrochemical Detector

A screen-printed electrode and molecular imprinting technology, which is applied in the direction of material electrochemical variables, etc., can solve the problems of large number of molecular imprinted membranes and waste of resources, and achieve the effects of high specificity, increased sensitivity, and strong detection specificity

Active Publication Date: 2020-06-09
JIANGNAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the molecularly imprinted detection methods reported in the existing reports are all based on disposable molecularly imprinted membranes. Although they are portable, they are a serious waste of resources, and the detection consumes a large number of molecularly imprinted membranes; at the same time, different detection objects are prepared in different ways. The obtained molecularly imprinted membrane is electrochemically detected, showing different data prediction rules, and the data models constructed are also different.

Method used

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  • A Portable AHL Molecularly Imprinted Screen Printed Electrochemical Detector
  • A Portable AHL Molecularly Imprinted Screen Printed Electrochemical Detector
  • A Portable AHL Molecularly Imprinted Screen Printed Electrochemical Detector

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0047] Example 1 Preparation of AHL Molecularly Imprinted Screen Printing Electrode

[0048] The schematic diagram of the preparation of AHL molecularly imprinted screen-printed electrode sheet is as follows figure 1 shown. The specific preparation method is as follows:

[0049] (1) Drop gold nano-crosses on the surface of the working screen printing electrode, drip coating method: drip-coat the configured gold nano-crosses solution on the surface of the working electrode, and dry it under an infrared lamp to complete the dripping of the gold nano-crosses add. The peak current value of the electrode on the CV diagram can be increased by dripping the gold nano cross, that is, the sensitivity of the electrode can be increased, such as image 3 shown.

[0050] (2) Modification of p-mercaptoaniline through gold sulfhydryl bonds, modification method: dip the screen-printed electrode coated with gold nanoparticles in a 50 mmol L-1 ethanol solution of p-mercaptoaniline, soak at r...

Embodiment 2

[0053] Example 2 Portable AHL Molecularly Imprinted Screen Printing Electrochemical Detector

[0054] The structure diagram of the portable AHL molecular imprinting electrochemical detector is as follows figure 2 As shown, the structure is as follows:

[0055] The molecularly imprinted screen-printed electrode obtained in Example 1 includes an electrode substrate, a terminal, an electrode connection, a working electrode, a counter electrode, and an insulating layer; the surface of the working electrode is electroplated with a molecularly imprinted polymer film; the electrode substrate is a primary The consumer substrate, working electrode, counter electrode and reference electrode are derived from the electrode connection to form a convex three-wire socket, which can be inserted into a portable electrochemical detector for detection and reading. The electrode substrate is printed with an electrode base body in which connecting terminals, electrode connecting wires and workin...

Embodiment 3

[0059] Example 3 standard curve

[0060] In a portable detector of the present invention, which is a dedicated portable detector for AHL, the standard curve between AHL concentration and peak current difference has been built into the detector program.

[0061] The drawing method of this standard curve: prepare different known concentrations (concentration range: 1 * 10 -13 ~1×10 -9 mol L -1 )’s AHL molecularly imprinted screen-printed electrode is inserted into the detector to detect the current peak value formed when electrons flow from the counter electrode, through the conductive liquid, and then flow back to the workstation from the working electrode. Take the AHL concentration as the abscissa, and the peak current value as the ordinate Coordinates, the standard curve is obtained, such as image 3 shown. The standard curve equation that obtains is: y=0.56439lgx+8.57752 (R 2 =0.94799), the detection limit was 10 -11 mol L -1 .

[0062] The obtained standard curve i...

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Abstract

The invention discloses a portable AHL molecular imprinting silk-screen printing electrochemical detector, and belongs to the technical field of rapid detection. According to the invention, the surface of a working electrode is coated with a gold nano cross in a dropping manner; then, an AHL molecular analogue is used as a template; a gold-containing electrode is put into an electropolymerizationsolution containing a monomer, a cross-linking agent, a template and an initiator by adopting a surface imprinting technology for electropolymerization; template molecules are eluted to obtain an AHLmolecular imprinting silk-screen printing electrode, a peak current difference value before and after adsorption is obtained based on the electrode in cooperation with specific electrochemical detection conditions, a standard curve is obtained by using the concentration of a to-be-detected substance and the peak current difference value, and the AHL content is calculated. The detector disclosed bythe invention can be used for directly detecting AHL molecules, has the advantages of sensitivity, rapidness, recycling for multiple times, high specificity and the like, is low in price, and is suitable for detecting the AHL molecules on a base layer or on site.

Description

technical field [0001] The invention relates to a portable AHL molecular imprinting screen printing electrochemical detector, which belongs to the technical field of rapid detection. Background technique [0002] Quorum sensing is a unique and diverse group behavior phenomenon that occurs when bacteria grow to a certain density and interact with each other, and perform gene expression and regulation. N-acyl homoserine lactone (AHL) compounds are the most important signal molecules in quorum sensing of Gram-negative bacteria, regulating the expression of many physiological characteristics genes. Rapid, simple and effective detection of whether bacteria can produce AHL has become an important means for in-depth research and understanding of bacterial quorum sensing. [0003] The principles of various detection methods for AHL molecules are different, and each method has its own advantages and disadvantages. However, due to the improvement of the sensitivity and accuracy of de...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N27/48
CPCG01N27/48
Inventor 孙秀兰纪剑黄鹤阳任奕婧皮付伟
Owner JIANGNAN UNIV
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