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A microfluidic chip and detection method for detecting nitrogen and phosphorus content in water

A microfluidic chip, nitrogen and phosphorus technology, applied in chemical instruments and methods, color/spectral characteristic measurement, laboratory containers, etc., can solve problems such as easy omission of important information, small change range, and few selection points, etc. Achieve the effects of simple process route, improved detection efficiency, and low reagent consumption

Active Publication Date: 2021-06-25
SHANDONG POLYTECHNIC
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] The current routine operation is basically based on the national standards GB 11894-89 and GB11893-89 and is moderately optimized according to experience. The range of change is small, the selection points are few, the representativeness is poor, and important information is easy to be missed; in addition, each sample needs to be processed separately, Independent detection and analysis, heavy workload

Method used

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  • A microfluidic chip and detection method for detecting nitrogen and phosphorus content in water
  • A microfluidic chip and detection method for detecting nitrogen and phosphorus content in water
  • A microfluidic chip and detection method for detecting nitrogen and phosphorus content in water

Examples

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Embodiment 1

[0045] This embodiment provides a microfluidic chip, its structure is as follows figure 1 As shown, the microfluidic chip includes four layers: the upper layer (layer 1), the diaphragm layer (layer 2), the lower layer (layer 3), and the support layer (layer 4), all of which are circular; among them:

[0046] The upper layer and the lower layer are respectively equipped with 8 channels, that is, 8 groups of mixing chambers, digestion chambers, color chambers and corresponding microchannels;

[0047] An upper layer reagent injection port 11 located in the center of the circle is provided in the upper layer and a corresponding upper layer liquid reservoir is provided; the eight upper layer mixing chambers 14 are respectively connected to the upper layer reagent injection port 11 through microchannels 171 of equal width, equal height and equal length. Corresponding overflow ports 181 are provided, and the straight-line distances between adjacent upper layer mixing chambers 14 are ...

Embodiment 2

[0064] In this example, the microfluidic chip provided in Example 1 is used to detect the nitrogen and phosphorus content in the water body. The difference is that the total nitrogen content is tested after being digested under the ultraviolet lamp for 15 minutes, and the frequency of the transducer is tested when the total phosphorus content is tested. is 100kHz, the power is 20W, and the processing time is 30s, the result is as follows image 3 As shown, based on the N and P detection results, the digestion solution ratio obtained when the width of the channel 371 is 200 microns is the optimal ratio.

Embodiment 3

[0066] In this example, the microfluidic chip provided in Example 1 is used to detect the nitrogen and phosphorus content in water. The difference is that: the mixed standard solution of nitrogen and phosphorus contains 3.5 mg of nitrogen and 0.4 mg of phosphorus per liter of solution; Test the total nitrogen content after digestion under the lamp for 15 minutes; when testing the total phosphorus content, the frequency of the transducer is 150 kHz, the power is 30 W, and the processing time is 30 s. than the range, the result is as image 3 As shown, based on the N and P detection results, the digestion solution ratio obtained when the width of the channel 371 is 200 microns is the optimal ratio.

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Abstract

The invention provides a microfluidic chip and a detection method for detecting the content of nitrogen and phosphorus in a water body. The microfluidic chip includes an upper layer, a membrane layer, a lower layer, and a support layer arranged sequentially from top to bottom, and the upper layer, the membrane layer, the lower layer, and the support layer are all circular; wherein: the upper layer is provided with an upper layer reagent Injection port, N upper-layer mixing chambers, N upper-layer digestion chambers, N upper-layer color chambers; the lower layer is provided with lower-layer reagent injection ports, N lower-layer mixing chambers, N lower-layer digestion chambers, and N lower-layer color chambers . A reference pool; the upper layer reagent injection port overlaps with the upper layer reagent injection port and is separated by a diaphragm layer. The invention also provides a detection method for detecting the content of nitrogen and phosphorus in the water body by using the microfluidic chip. The microfluidic chip of the present invention can realize rapid, convenient and controllable preparation of digestion solutions with different concentrations.

Description

technical field [0001] The invention relates to a microfluidic chip for detecting nitrogen and phosphorus content in water bodies and a detection method, belonging to the technical field of microfluidic chips. Background technique [0002] A large amount of domestic sewage, farmland drainage or industrial wastewater is discharged into the water body, increasing the content of nitrogen and phosphorus in the water, causing a large number of organisms or microorganisms to multiply, consuming dissolved oxygen in the water, and deteriorating the quality of the water body. In addition, the content of total nitrogen and total phosphorus in different water sources such as lakes, reservoirs, aquaculture wastewater, and sea water is also very different. At present, there is no standard detection method suitable for the detection of nitrogen and phosphorus in various water bodies. Among them, the addition amount and addition ratio of potassium persulfate and sodium hydroxide in the dig...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N21/78G01N21/31B01L3/00
CPCB01L3/5027G01N21/31G01N21/78
Inventor 贾俊刚顾业明
Owner SHANDONG POLYTECHNIC
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