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Lily and rhizoma anemarrhenae extract with antidepressant effect

An Anemarrhena Anemarrhena extract and antidepressant technology are applied in the preparation of antidepressant drugs. The preparation field of Anemarrhena Anemarrhena extract can solve problems such as research gaps in the extraction of furostanol saponins, achieve good behavioral improvement, and improve brain function. The effect of endotransmitter content

Inactive Publication Date: 2019-07-09
JINING MEDICAL UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The inventor believes that the extraction methods for saponins in the prior art are mostly solvent extraction methods, and the extraction research for furostanol saponins is relatively blank

Method used

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  • Lily and rhizoma anemarrhenae extract with antidepressant effect
  • Lily and rhizoma anemarrhenae extract with antidepressant effect
  • Lily and rhizoma anemarrhenae extract with antidepressant effect

Examples

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preparation example Construction

[0022] The first aspect of the present disclosure provides a preparation method of anemarrhena lily extract, the preparation method comprising the following steps: adding an alcohol solution to lily and anemarrhena, obtaining an alcohol extract by percolation, and concentrating under reduced pressure to remove the alcohol extract The organic phase in the extract is obtained; the extract is dissolved in water, and the organic phase is added successively for extraction, the organic phase part is retained, and the solvent is removed to obtain a crude extraction, and the crude extract is transformed into Sphingomonas ginsenosides ( Sphingomonas ginsenosidimutans) fermentation to obtain the Anemarrhena lily extract.

[0023] Preferably, the above preparation method further includes the step of column chromatography purification.

[0024] In some embodiments, the mass ratio of Lily to Zhimu is 4-6:1-3.

[0025] In some embodiments, the alcohol solution is 60-80% ethanol.

[0026] ...

Embodiment 1

[0037] The preparation of embodiment 1 extract

[0038] Weigh 50g lily lily and 20g anemarrhena crude powder and mix them with filter paper, place them in a percolation cylinder, add 10 times the volume of 70% ethanol for percolation extraction, the percolation speed is 3mL / min, and obtain the alcohol extraction liquid. Concentrate under reduced pressure to remove ethanol in the alcohol extract to obtain the extract.

[0039] Weigh 3 mg of extract and add 20 mL of water to dissolve, add 15 mL of ethyl acetate to the solution for extraction, 5 mL each time, and combine the extracts to obtain the ethyl acetate part. Continue to add 15 mL of chloroform to the remaining aqueous phase solution for extraction, 5 mL each time, and combine the extracts to obtain the chloroform fraction. The organic solvents in the ethyl acetate part and the chloroform part were respectively removed by distillation under reduced pressure to obtain a crude extract of Anemarrhena lily.

[0040] Weigh ...

Embodiment 2

[0041] The preparation of embodiment 2 extract

[0042]Inoculate transformed Sphingomonas ginsenosidimutans (Sphingomonas ginsenosidimutans) CICC 10895 into R2A medium, and activate it by culturing it under aerobic conditions at 37°C for 24 hours. Repeat this operation to obtain secondary activated strains, and select colonies with good vigor for expansion. Cultivate the seed solution to obtain the strain.

[0043] The crude extract of Anemarrhena lily prepared in Example 1 was added to R2A medium to form a 1.2 mg / ml solution. The activated strain seed solution was added to the prepared solution at an inoculum amount of 3%, and cultured under aerobic conditions at 28° C. for 7 days to obtain a fermentation product.

[0044] Filter the fermentation product through a 0.5 μm filter membrane, weigh 0.5 mg of the fermentation product and add 6 mL of methanol to dissolve it completely after drying, add a small amount of column chromatography silica gel to mix the sample, and carry ...

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Abstract

The invention belongs to the technical field of natural medicine extracts and particularly relates to a lily and rhizoma anemarrhenae extract and a preparation method and application thereof. The research on antidepressant activity of rhizoma anemarrhenae in the field indicates that total saponins of rhizoma anemarrhenae have antidepressant activity and can reverse hyperfunction of hypothalamic-pituitary-adrenal axis (HPA) by increasing neurotransmitters in brain. The previous research of the inventor proves that the furostanol saponin in the extracts of lily and rhizoma anemarrhenae has goodantidepressant activity. The lily and rhizoma anemarrhenae extract has the good extraction effect on furostanol saponin, and is verified by an animal model so that the extract can effectively improvethe behaviors of depressed animals, the secretion of neurotransmitters in the brain of the animals can be increased, and the extract can be expected to be an antidepressant drug with a good prospect.

Description

technical field [0001] The disclosure belongs to the technical field of natural medicine extracts, and in particular relates to a preparation method of Anemarrhena lily extract and its application in the preparation of antidepressant medicines. Background technique [0002] The information disclosed in this Background section is only intended to increase the understanding of the general background of the disclosure, and is not necessarily to be taken as an acknowledgment or any form of suggestion that the information constitutes prior art that is already known to those skilled in the art. [0003] Lily Anemarrhena Decoction, from the volume of "Synopsis of the Golden Chamber". Lily, moistens the lungs and clears the heart, replenishes qi and calms the nerves; Anemarrhena, clears heat and promotes body fluid, relieves restlessness and moistens dryness. The solvent spring water in this prescription is special, and ancient herbal medicine believes that it has the effects of be...

Claims

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Application Information

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IPC IPC(8): A61K36/8967A61P25/24
CPCA61K36/8964A61K36/8967A61K2236/19A61K2236/333A61K2236/55A61P25/24A61K2300/00
Inventor 钟鸣张敏娜王婷婷郑公朴王光辉周采菊
Owner JINING MEDICAL UNIV
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