Method for preparing biostimulant by utilizing amino acid fermentation tail liquid

A technology of biostimulants and fermentation tails, applied in the fields of chemicals for biological control, botanical equipment and methods, applications, etc., can solve problems such as increasing the burden on enterprises, corrosion, etc., to promote plant growth and improve hydrolysis rate , improve the ability of enzyme production and the effect of cell membrane permeability

Inactive Publication Date: 2019-07-09
许传高
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The applicant's previous patented technology "2014105587445, a method for producing liquid fertilizer by using glutamic acid wastewater" discloses a method for preparing liquid fertilizer by using amino acid fermentation wastewater. , using high-concentration hydrochloric acid of 6-8mol / L, stirred and hydrolyzed at 60°C for 12-16 hours, and prepared amino acids by hydrolyzing bacterial protein. This method can obtain a hydrolysis rate of about 50%, but high-concentration hydrochloric acid is harmful to The equipment caused serious corrosion, and the equipment was updated quickly, which increased the burden on the enterprise

Method used

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  • Method for preparing biostimulant by utilizing amino acid fermentation tail liquid

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0029] A method for preparing a biostimulant using amino acid fermentation tail liquid, comprising the steps of:

[0030] Step 1) Separation, drying and crushing: the amino acid fermentation tail liquid is separated by a disc separator with a rotation speed of 5000 rpm and a centrifugation time of 3 minutes to collect bacterial protein precipitates and waste mother liquor, then dry the bacterial precipitates, and then The pulverizer is crushed into bacterial protein powder;

[0031] Step 2) Concentration: Concentrate the waste mother liquor to one-fifth of the original volume to obtain a concentrate;

[0032] Step 3) Microbial enzymatic hydrolysis treatment: Inoculate Aspergillus niger ATCC 16404 into the medium of the seed tank for cultivation, the temperature is controlled at 30°C, and the cultivation is carried out until the bacterial concentration is 1.0×10 8 cfu / ml, to obtain the seed liquid; the composition of the seed tank culture medium is: 20g / L glucose, 6g / L corn st...

Embodiment 2

[0036] A method for preparing a biostimulant using amino acid fermentation tail liquid, comprising the steps of:

[0037] Step 1) Separation, drying and crushing: the amino acid fermentation tail liquid is separated by a disc separator with a rotation speed of 5000 rpm and a centrifugation time of 3 minutes to collect bacterial protein precipitates and waste mother liquor, then dry the bacterial precipitates, and then The pulverizer is crushed into bacterial protein powder;

[0038] Step 2) Concentration: Concentrate the waste mother liquor to one-fifth of the original volume to obtain a concentrate;

[0039] Step 3) Microbial enzymatic hydrolysis treatment: Inoculate Aspergillus niger ATCC 16404 into the medium of the seed tank for cultivation, the temperature is controlled at 30°C, and the cultivation is carried out until the bacterial concentration is 1.0×10 8 cfu / ml to obtain seed liquid; the composition of the seed tank culture medium is: 20g / L glucose, 6g / L corn steep l...

Embodiment 3

[0043] Effects of different treatment methods on the degree of proteolysis of bacteria:

[0044] The components in the amino acid fermentation tail liquid of the present invention are as follows (dry basis %): bacterial protein 35.2, amino acid 14.1, polysaccharides 5.3, ammonia nitrogen 9.9, sulfur 9.0, calcium 3.2, potassium 1.3, iron 0.9, and the balance is other .

[0045] Set group:

[0046] Take embodiment 1 as experimental group;

[0047] Control group 1: use 6mol / L hydrochloric acid to hydrolyze the bacterial protein with stirring and hydrolysis at a temperature of 60°C for 16 hours, and the rest of the operations are the same as in Example 1;

[0048] Control group 2: 2mol / L hydrochloric acid was used to hydrolyze the bacterial protein with stirring and hydrolysis at 60°C for 32 hours, and the rest of the operations were the same as in Example 1;

[0049] Control group 3: do not add tourmaline powder, all the other operations are the same as embodiment 1;

[0050]...

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Abstract

The invention belongs to the field of amino acid fermentation tail liquid treatment, and discloses a method for preparing biostimulant by using amino acid fermentation tail liquid, which comprises thefollowing steps: 1) separation, drying and crushing, 2) concentration, 3) microbial enzymolysis treatment, 4) seaweed extract preparation, and 5) biostimulant preparation. The fermentation tail liquid is treated, and a concentrated liquid, an enzymatic hydrolysate, humic acid and a seaweed extract are reasonably combined to prepare the biostimulant, so that the physiological process of crops canbe regulated and controlled, the plant growth is promoted, and the product quality is improved.

Description

technical field [0001] The invention belongs to the field of amino acid fermentation tail liquid treatment, and in particular relates to a method for preparing biostimulants by using amino acid fermentation tail liquid. Background technique [0002] Biostimulants are generally a type of product that is naturally derived, contains biologically active substances, and plays a good role in promoting plants. It is neither a pesticide nor a traditional fertilizer; the target of a biostimulant is the crop itself, which can improve the quality of the plant. The physiological and biochemical state of the plant plays a very good role in promoting the physiological system inside the plant, improving the effect of pesticides and fertilizer utilization, improving the level of crop resistance to adversity, and improving the final yield of crops and the quality of agricultural products. In 2013, a survey and research report by the European Union divided biostimulants into 8 categories, inc...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01N65/00A01N63/04A01N61/00A01N37/44A01P21/00
CPCA01N65/00A01N61/00A01N37/44A01N63/30
Inventor 赵兰坤孙钦波赵春晓程士清时夫龙
Owner 许传高
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