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Method for constructing tau protein content detection system in alzheimer disease blood

A technology for Alzheimer's disease and protein content, applied in the medical field, can solve the problems of slow analysis speed, inconvenient use, and poor sensitivity of detection technology, and achieve enhanced specific adsorption capacity, fast analysis speed, and low cost Effect

Inactive Publication Date: 2019-07-05
WUHAN UNIV OF SCI & TECH
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Problems solved by technology

[0006] (1) In the existing detection technology of tau protein content in Alzheimer's disease blood, the specificity is high and the sensitivity is poor, and the existing technology has research on the detection of tau protein by electrochemical biosensor, but there is no combination Research on the body-antibody sandwich model Electrochemical biosensors are applied to the detection of blood tau protein content in Alzheimer's disease, resulting in slow analysis speed, inconvenient use, and high cost of existing detection technologies

Method used

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  • Method for constructing tau protein content detection system in alzheimer disease blood
  • Method for constructing tau protein content detection system in alzheimer disease blood
  • Method for constructing tau protein content detection system in alzheimer disease blood

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preparation example Construction

[0047] (2) Preparation and characterization of gold nanoparticles: Gold nanoparticles generally modify electrodes by electrostatic adsorption, covalent bonding, electrochemical deposition, etc. The modified electrochemical interface is the key to enhancing the signal response of electrochemical biosensors. In the present invention, gold nanoparticles are used as signal amplification materials of electrochemical biosensors. The high surface energy and interface controllability of gold nanoparticles improve the immobilization rate of modified molecules on the surface, enhance the analysis signal, and promote good biocompatibility. charge transfer between biomolecules and electrodes. Use a transmission electron microscope to observe the shape and size of the gold nanoparticles, and determine whether the prepared gold nanoparticles are successful through characterization.

[0048] (3) Immobilizing antibodies: 3-mercaptopropionic acid is used to modify the surface of the gold elect...

Embodiment

[0052] Examples (such as Figure 1-Figure 9 )

[0053] The method for constructing a tau protein content detection system in Alzheimer's disease blood by using an electrochemical biosensor provided by the embodiment of the present invention includes:

[0054] 1) Preparation of electrochemical biosensor in aptamer-antibody sandwich mode:

[0055] (1) Electrode pretreatment: use aluminum powder to polish, ethanol and pure water to ultrasonically clean the electrode surface for 3 minutes each. Immerse the electrode in 2mol / L potassium hydroxide solution, concentrated sulfuric acid diluted 20 times and concentrated nitric acid solution for 15 minutes respectively. The electrode is activated by cyclic voltammetry in 0.5mol / L sulfuric acid solution, the scanning range is 0.4-1.7V, and the scanning is repeated until a stable cyclic voltammetry is reached.

[0056] (2) Preparation and characterization of gold nanoparticles: Add 18mL pure water into a beaker, then add 0.01mol / L tetr...

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Abstract

The invention belongs to the technical field of the medical science, and discloses a method for constructing a tau protein content detection system in alzheimer disease blood. According to the method,an electrode surface modification technology is applied, the antibody is modified to an electrode surface, aptamer is assembled on gold-nanoparticles through covalent combination, the tau protein iscombined to the electrode surface by utilizing the specificity binding of the antibody and the tau protein, and specificity adsorption thereof is enhanced under the effect of the aptamer, thereby constructing an electrochemical biosensor in an aptamer-antibody sandwich mode so as to detect the tau protein content in the alzheimer disease blood. The biochemical biosensor application is extensive, compared with a traditional enzyme linked immunosorbent assay (ELISA) method, the electrochemical biosensor has the advantages of being simple in preparation, good in stability, high in analysis speed,convenient to use and low in cost.

Description

technical field [0001] The invention belongs to the technical field of medicine, and in particular relates to a method for constructing a tau protein content detection system in Alzheimer's disease blood. Background technique [0002] Currently, the closest prior art: [0003] Alzheimer's disease (AD) is the most common chronic neurodegenerative disease, a key progress in the field of AD research is to prove that two classic neuropathic changes senile plaques and neurofibrillary tangles are The final outcome in the pathological process of AD. Among them, the main component of senile plaques is β-amyloid protein (Amyloid β-protein, referred to as Aβ), and the main component of neurofibrillary tangles is tau protein. [0004] At present, the diagnosis of AD still needs autopsy to find senile plaques, neurofibrillary tangles and selective reduction of neurons in specific parts, which cannot be done for living patients. Deposition of tau protein is the main pathological basis...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N27/327G01N27/48
CPCG01N27/3278G01N27/48
Inventor 郭振中税冰清陶丹
Owner WUHAN UNIV OF SCI & TECH
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