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Micro-ecology compound traditional Chinese medicine preparation and preparing method and application thereof

A technology of traditional Chinese medicine microecology and compound preparations, which is applied in the direction of medical formulas, anti-infectives, drug combinations, etc., can solve problems such as the core competitiveness of pig farms and the impact of economic benefits, the reduction of host immune function, and the limitations of antibiotic therapy. Antibiotic residue problem, excellent synergistic effect, good therapeutic effect

Inactive Publication Date: 2019-07-05
山东宝来利来生物工程股份有限公司
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In addition, in some large-scale pig farms with relatively extensive management, the incidence of inflammation in weaned sows is as high as 50-80%. The core competitiveness and economic benefits of the pig farm have a greater impact
[0005] At present, antibiotic treatment is still the main means of controlling sow reproductive tract diseases, especially in preventing acute infection from becoming chronic infection and removing possible infections caused by other pathogenic bacteria. Treatment causes changes in the flora in the sow. While eliminating sensitive bacteria, drug-resistant strains can survive and reproduce, and the bacterial resistance is serious; some antibacterial drugs can damage the function of phagocytic cells, resulting in a decrease in the immune function of the host; On the other hand, antibiotic remnants are a serious hazard to consumers' health, and antibiotic therapy is increasingly limited

Method used

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  • Micro-ecology compound traditional Chinese medicine preparation and preparing method and application thereof

Examples

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Effect test

preparation example 1

[0073] The specific preparation method of the freeze-dried powder of Lactobacillus acidophilus BLCC2-0111 is as follows:

[0074] (I) Strains: Lactobacillus acidophilus BLCC2-0111 was selected;

[0075] (II) Slant culture: inoculate one ring of strains on a solid slant medium, and cultivate at 37° C. for 18 hours to obtain slant strains;

[0076] (Ⅲ) First-class seed liquid: pick a ring of slant strains, insert it into 1000mL sterilized liquid seed medium, and culture it at 37°C for 16 hours to obtain the first-class seed liquid;

[0077] (Ⅳ) Seed tank cultivation: With an inoculum size of 2% (v / v), connect the primary seed liquid in a sterilized seed tank containing 300L liquid seed medium, and culture it statically for 10 h at 37°C , to prepare the seed solution;

[0078] (Ⅴ) Fermentation tank culture: with the inoculum amount of 2% (v / v), the seed liquid obtained from the fermentation of the seed tank was connected to the sterilized liquid fermentation medium of the ferme...

Embodiment 1

[0086] Example 1 Screening of lactic acid bacteria

[0087] 1. Material method

[0088] 1.1 Strains

[0089] 1.1.1 Lactic acid bacteria

[0090] Select Enterococcus faecium BLFC-3-7, Lactobacillus plantarum LP, Lactobacillus acidophilus BLCC2-0111 as candidate strains. The above three strains of lactic acid bacteria were provided by the Bacteria Resource Collection Center of the Bioengineering Research Institute of Shandong Baolai Lilai Bioengineering Co., Ltd.

[0091] 1.1.2 Sow reproductive tract pathogenic strains

[0092] Three strains of pathogenic bacteria isolated from the reproductive tract of sows in a pig farm in Tai'an City, Shandong Province: Escherichia coli, Staphylococcus aureus, and Streptococcus pyogenes.

[0093] 1.2 Medium:

[0094] Lactic acid bacteria medium: MRS medium.

[0095] Pathogen culture medium: BHI brain heart infusion medium.

[0096] 1.3 Test method

[0097] 1.3.1 Preparation of lactic acid bacteria fermentation broth

[0098] Under a...

Embodiment 2

[0108] Example 2 Determination of antibacterial active substances in fermentation product of Lactobacillus acidophilus BLCC2-0111 and crude extraction of antibacterial substances

[0109] 2.1 Determination of antibacterial active substances in the fermentation product of Lactobacillus acidophilus BLCC2-0111

[0110] 2.1.1 Acid inhibition and exclusion test: Since lactic acid bacteria produce a large amount of lactic acid during the fermentation process, the pH value of the fermentation supernatant of lactic acid bacteria can reach about 4.0, and the inhibitory effect on the indicator bacteria may also be the lactic acid secreted by lactic acid bacteria. In order to exclude the interference of lactic acid, the pH values ​​of lactic acid-adjusted distilled water and lactic acid-adjusted MRS medium were 3.0, 4.0, 5.0, and 6.0, respectively, as controls. Take 1 g of the lyophilized powder of Lactobacillus acidophilus BLCC2-0111 and its fermentation product (taken from Preparati...

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Abstract

The invention provides a micro-ecology compound traditional Chinese medicine preparation and a preparing method and application thereof. The micro-ecology compound traditional Chinese medicine preparation comprises a micro-ecology compound traditional Chinese medicine composition and is characterized in that the composition comprises probiotics and compound traditional Chinese medicine; the probiotics are Lactobacillus acidophilus and / or fermentation products thereof; the compound traditional Chinese medicine comprises rhizoma coptidis, folium mori, taraxacum mongolicum and fructus forsythia.The micro-ecology compound traditional Chinese medicine composition and the preparation thereof are good in antibacterial activity and capable of well preventing and treating sow reproductive tract infection.

Description

technical field [0001] The invention relates to the technical field of microecological preparations, in particular to a traditional Chinese medicine microecological compound preparation and its preparation method and application. Background technique [0002] The information disclosed in this background section is only intended to increase the understanding of the general background of the present invention, and is not necessarily taken as an acknowledgment or any form of suggestion that the information constitutes the prior art already known to those skilled in the art. [0003] Sow reproductive tract (including uterus and vagina) disease is a common and frequently-occurring disease in pig production. In the process of sow breeding, due to factors such as poor feeding and production environmental conditions, nutritional imbalance, strong stress response, and unhygienic fertilization, the corresponding symptoms of reproductive tract inflammation in sows to be delivered and p...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K36/718A61P31/00A61P15/00A61K35/747
CPCA61K35/747A61K36/288A61K36/605A61K36/634A61K36/718A61K2236/331A61K2236/39A61P15/00A61P31/00A61K2300/00
Inventor 王春凤单宝龙张志焱于佳民赵倩孙明杰李金敏陈雷谷巍徐海燕王红
Owner 山东宝来利来生物工程股份有限公司
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