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Method for assisting in detecting growth characters by using CNV molecular marker of cattle PLAG1 gene and application thereof

A technology of PLAG1 and yellow cattle, which is applied in the field of real-time fluorescent quantitative PCR technology to detect the CNV markers of yellow cattle PLAG1 gene, can solve the problem of clear detailed mechanism research, and achieve the effects of convenient molecular marker-assisted selection, excellent genetic resources, and low cost

Active Publication Date: 2019-06-28
NORTHWEST A & F UNIV
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Problems solved by technology

However, the detailed mechanism of the PLAG family, especially the PLAG1 gene, in the growth and development of cattle has not been studied clearly

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  • Method for assisting in detecting growth characters by using CNV molecular marker of cattle PLAG1 gene and application thereof
  • Method for assisting in detecting growth characters by using CNV molecular marker of cattle PLAG1 gene and application thereof
  • Method for assisting in detecting growth characters by using CNV molecular marker of cattle PLAG1 gene and application thereof

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Embodiment Construction

[0027] The invention will be described in detail below in conjunction with the accompanying drawings and embodiments, and the embodiments are only for explaining the present invention, rather than limiting the protection scope of the present invention.

[0028] In the previous cattle genome resequencing research, it was found that the copy number variation occurred in the 37024 to 38623 positions of the bovine PLAG1 genome sequence. Therefore, based on the physiological role of the PLAG1 gene and the regulation mechanism of CNV, the copy number variation and growth of the PLAG1 gene were studied. The correlation of traits is very necessary and can provide an important theoretical basis for molecular breeding of cattle.

[0029] In the present invention, specific primers are designed according to the region of copy number variation in the bovine PLAG1 genome sequence obtained by resequencing as a template, and then qPCR amplification is performed using the yellow cattle genome D...

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Abstract

The invention discloses a method for assisting in detecting growth characters by using a CNV molecular marker of cattle PLAG1 genes and an application thereof. The method for assisting in detecting the growth characters by using the CNV molecular marker of the cattle PLAG1 genes comprises the following steps of: respectively amplifying partial fragments of a PLAG1 gene copy number variation regionand a reference gene BTF3 by using a cattle genome DNA as a template through a real-time fluorescence quantitative PCR method, and dividing the quantitative results into multiple copy type and normaltype by 2-delta delta Ct method, thereby identifying copy number variation type of individual PLAG1 gene of a cattle. The copy number variation closely related to the growth characters of the cattleis detected on a DNA level, and the copy number variation can be used as an important candidate molecular marker for marker-assisted selection of the growth characters of Chinese cattles and is used for accelerating the breeding work of the marker-assisted selection of the cattle.

Description

technical field [0001] The invention relates to the field of livestock molecular biology detection, in particular to a method for detecting the CNV marker of yellow cattle PLAG1 gene based on real-time fluorescent quantitative PCR (qPCR) technology. The method uses genomic DNA as a template, uses real-time quantitative PCR, and uses the BTF3 gene as a reference to determine the type of copy number variation of an individual based on the -ΔΔCt value. Background technique [0002] With the continuous development of genomics and bioinformatics, the direction of beef cattle breeding is developing from phenotypic breeding to molecular breeding. Molecular breeding, that is, molecular mark-assisted selection (MAS), is a breeding technology that uses DNA molecular markers to select genetic resources or breeding materials and improve the comprehensive traits of livestock and poultry. In livestock and poultry breeding, through the selection of DNA markers that are closely related to ...

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Application Information

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IPC IPC(8): C12Q1/6888C12Q1/6851
CPCY02A40/70
Inventor 黄永震何盼贺花王献伟张子敬徐泽君茹宝瑞王二耀雷初朝陈宏胡沈荣
Owner NORTHWEST A & F UNIV
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