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Molecular identification method of mouse species

A technology of molecular identification and rodent species, which is applied in the determination/inspection of microorganisms, biochemical equipment and methods, etc., can solve the problems of error-prone and inability to identify other rodent species, and achieve easy identification, obvious morphological characteristics, and versatility strong effect

Inactive Publication Date: 2019-06-28
PLANT PROTECTION RES INST OF GUANGDONG ACADEMY OF AGRI SCI
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Problems solved by technology

However, this invention is limited to identifying whether the samples to be tested are four kinds of mice: yellow-breasted rat, Mus musculus, Rattus norvegicus and Apodemus nigra, and cannot identify other rat species; and only the cyt b gene sequence is selected as the basis for identification. An error occurred

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  • Molecular identification method of mouse species
  • Molecular identification method of mouse species

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Embodiment

[0033] Yellow-haired rat (Rattus losea) and yellow-breasted rat (Rattus tanezumi) are closely related species, both belong to the genus Rat (Rattus) of the Muridae family (Muridae), and are easily confused in shape. Take the farmland of Xinhui District, Jiangmen City, Guangdong Province Take a captured mouse as an example, which was identified as a yellow-haired rat by morphological identification.

[0034] However, different results may be obtained by using the molecular identification method of the present invention;

[0035] The identification process is as follows:

[0036] (1) Cut 1-2 toes of the mouse into pieces, and extract genomic DNA according to the instructions of the MiniBEST Universal Genomic DNA Extraction Kit Ver.5.0 (TaKaRa) kit;

[0037] (2) Using the aforementioned primers (SEQ ID NO.1-SEQ ID NO.4) to amplify CO I and cyt b gene fragments, the PCR reaction conditions for CO I gene amplification are: 95°C pre-denaturation for 5 minutes, 95°C Denaturation fo...

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Abstract

The invention discloses a molecular identification method of a mouse species, which comprises the following steps of: extracting genome DNA of the mouse species to be detected; respectively amplifyingmitochondrial cytochrome C oxidase subunit I gene and mitochondrial cytochrome b gene by using specific primers, and then sequencing; carrying out Blast nucleic acid sequence homology search on the NCBI website, wherein the sequence with the highest homology in the comparison result is the mouse species with the closest genetic relationship. According to the molecular identification method of themouse species, a PCR primer and a reaction program have strong universality, the method is simple and easy to operate, the mouse identification can be quickly and accurately performed, and the defectof morphological identification is overcome; meanwhile, the accuracy of the identification result is improved by mutually verifying two gene sequences of CO I and cyt b. In addition to taxonomicallyidentifying the mouse species, the molecular identification method of the invention is important for the study of biological genetic diversity and phylogenetic evolution by analyzing the sequence characteristics of the CO I and cyt b genes between species.

Description

technical field [0001] The invention relates to an accurate and fast mouse species molecular identification method. Background technique [0002] Rat species identification is an important part of rodent research. At present, most of the identification is based on morphological characteristics, such as appearance characteristics, skull characteristics, and tooth characteristics. [0003] However, due to the influence of many factors such as habitat conditions, growth and development stages, and organ morphology, the appearance identification characteristics of the same species of mice sometimes vary and it is difficult to accurately identify them, such as the albino brown rat (Rattus norvegicus). Some closely related species and cryptic species are very similar in morphological characteristics and cannot be distinguished accurately. Damaged or incomplete morphological features of the sample, material corruption and other factors are also prone to misjudgment; and the identi...

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Application Information

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IPC IPC(8): C12Q1/6888C12Q1/6806
Inventor 姚丹丹姜洪雪冯志勇
Owner PLANT PROTECTION RES INST OF GUANGDONG ACADEMY OF AGRI SCI
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