Real-time fluorescent quantitative PCR (polymerase chain reaction) kit for assisting diagnosis of tuberculous pleuritis and application thereof

Abstract

The invention discloses a real-time fluorescent quantitative PCR (polymerase chain reaction) kit for assisting diagnosis of tuberculous pleuritis and application thereof. The kit comprises a primer and a probe which are used for detecting mycobacterium tuberculosis specific small RNA fragments (Mtb-ms-1 and Mtb-ms-2), and a sequence of Mtb-ms-1 is shown as SEQ ID NO.1, and a sequence of Mtb-ms-2 is shown as SEQ ID NO.2. Total RNA of little pleural fluid of a patient with pleuritis is extracted, mycobacterium tuberculosis specific small RNA segments in the total RNA are reversely transcribed into complementary cDNA, and a specific primer probe is applied for real-time fluorescent quantitative polymerase chain reaction detection, so that the kit is high in diagnosis sensitivity and specificity, can remarkably differentiate tuberculous pleuritis and a comparison group and is better than diagnosis methods like mycobacterium tuberculosis culture, anti-fast smear staining and DNA detection.

Description

technical field [0001] The invention relates to a kit for detecting specific small RNA fragments encoded by Mycobacterium tuberculosis in pleural effusion of patients with tuberculous pleurisy based on real-time fluorescent quantitative polymerase chain reaction method, which is applied to the auxiliary diagnosis of tuberculous pleurisy. The invention belongs to the technical field of medicine. Background technique [0002] Tuberculosis is one of the infectious diseases with the highest morbidity and impact worldwide, and about 1 / 4 of it is extrapulmonary tuberculosis. Tuberculous pleurisy is the second most common cause of exudative pleurisy, second only to lymphatic tuberculosis. Traditional diagnostic methods for tuberculous pleurisy include Mycobacterium tuberculosis culture, acid-fast smear staining, pleural biopsy, etc. The diagnostic sensitivity is low and time-consuming, which often leads to delays in treatment timing. In recent years, the molecular detection techn...

AI Technical Summary

Problems solved by technology

[0004] The present invention overcomes the defects of low diagnostic sensitivity, long time consumption and high cost of existing laboratory diagnostic methods for tuberculous pleurisy, and provides a sensitive, specific and rapid molecular biological detection method, which has a high sensitivity for diagnosing tuberculous pleurisy. It is superior to traditional tuberculosis detection and tuberculosis DNA detection methods, so it can be used as a new diagnostic method for tuberculous pleurisy

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