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Cell separation system and method

A separation system and cell technology, applied in the field of cell separation, can solve the problems of high cost, affect the separation effect, and less harvest of stem cells, and achieve the effect of improving efficiency

Active Publication Date: 2019-06-21
北京泰盛生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although these separation techniques can achieve the separation of specific types of cells, the complex operation, low separation efficiency and high cost limit their large-scale clinical application, and many existing experimental studies are based on a filter membrane and Separation with the same pore size will lead to the loss of cells of a specific size; when the sample quality is poor, the filter membrane is prone to clogging, which affects the separation effect
Its separation efficiency is low, the amount of harvested stem cells is small, and it is time-consuming and laborious

Method used

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  • Cell separation system and method
  • Cell separation system and method

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specific Embodiment approach 1

[0036] Combine below Figure 1-11 In this embodiment, a cell separation system includes a base 1, a separation cylinder assembly 2, a screening assembly 3, a power assembly 4, a reciprocating drive mechanism 5, a transmission wheel assembly 6, a stirring assembly 7 and an anti-clogging assembly 8. The separation cylinder assembly 2 is fixedly connected to the base 1, the screening assembly 3 is fixedly connected between the base 1 and the separation cylinder assembly 2, the power assembly 4 is fixedly connected to the upper end of the separation cylinder assembly 2, and the reciprocating drive mechanism 5 is fixedly connected to the separating The upper end of the cylinder assembly 2, the power assembly 4 is slidingly connected to the reciprocating drive mechanism 5, two transmission wheel assemblies 6 are provided, and the two transmission wheel assemblies 6 are symmetrically fixedly connected to the separation cylinder assembly 2, and the reciprocating drive mechanism 5 The ...

specific Embodiment approach 2

[0038] Combine below Figure 1-11 To illustrate this embodiment, the separation cylinder assembly 2 includes a separation cylinder body 2-1, a screen 2-2, an arc-shaped seat plate 2-3 and support legs 2-4; the front end of the separation cylinder body 2-1 is provided with The discharge through hole, the inner side of the discharge through hole is fixedly connected to the screen 2-2, the screen 2-2 is provided with a filter hole I, and the lower end of the separation cylinder body 2-1 is symmetrically fixedly connected to two arc-shaped seat plates 2- 3. The lower ends of the two arc-shaped seat plates 2-3 are respectively fixedly connected to the two support legs 2-4, and the four support legs 2-4 are all fixedly connected to the base 1. When the separation cylinder assembly 2 is in use, the mixed liquid is poured into the separation cylinder body 2-1, and the separated cells and waste liquid are discharged through the filter hole I on the screen 2-2.

specific Embodiment approach 3

[0040] Combine below Figure 1-11 To illustrate this embodiment, the screening assembly 3 includes a vertical plate 3-1, a horizontal plate 3-2, a first sieve plate 3-3, a second sieve plate 3-4, a side plate 3-5, and an L-shaped seat Plate 3-6, the first collection box 3-7 and the second collection box 3-8; the two ends of the vertical plate 3-1 are respectively fixedly connected to the separation cylinder body 2-1 and the base 1, and the front end of the vertical plate 3-1 Two horizontal plates 3-2 are fixedly connected, the first sieve plate 3-3 and the second sieve plate 3-4 are respectively fixedly connected to the two horizontal plates 3-2, and the second sieve plate 3-4 is located on the first sieve plate Below 3-3, the first sieve plate 3-3 is inclined to the lower left, the second sieve plate 3-4 is inclined to the lower right, and the left and right ends of the horizontal plate 3-2 are fixedly connected to a side plate 3-5 respectively. An L-shaped seat plate 3-6 is...

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Abstract

The invention relates to a cell separation system and method and relates to the technical field of cell separation. The cell separation system comprises a base, a separation cylinder component, a screening component, a power component, a reciprocating drive mechanism, drive wheel components, a stirring component and a blocking-preventing component and is characterized in that the separation cylinder component is fixedly connected to the base, the screening component is fixedly connected between the base and the separation cylinder component, the power component is fixedly connected to the upper end of the separation cylinder component, the reciprocating drive mechanism is fixedly connected to the upper end inside the separation cylinder component, the power component is connected to the reciprocating drive mechanism in a slide fit manner, and the two drive wheel components are symmetrically fixedly connected to the separation cylinder component. The cell separation system has the advantages that cells can be sufficiently separated from mixed liquid, the cells are filtered and separated in a gradient manner through filter holes with gradually reduced hole diameters, and a good retention rate can be kept for cells of different sizes; screen blocking can be effectively avoided.

Description

technical field [0001] The invention relates to the technical field of cell separation, in particular to a cell separation system and a cell separation method. Background technique [0002] Cell sorting is the most important technical method and necessary prerequisite for cell biology and molecular biology. At present, the technology is relatively mature and widely used, mainly including: density gradient centrifugation, immune density centrifugation, immunomagnetic bead method and flow cytometry Wait. Although these separation techniques can achieve the separation of specific types of cells, the complex operation, low separation efficiency and high cost limit their large-scale clinical application, and many existing experimental studies are based on a filter membrane and Separation with the same pore size will lead to the loss of cells of a specific size; when the quality of the sample is poor, the filter membrane is prone to clogging, which affects the separation effect. ...

Claims

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Application Information

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IPC IPC(8): C12M1/12C12M1/02C12M1/00C12N5/00
Inventor 何春兰董银
Owner 北京泰盛生物科技有限公司
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