Detection method of paper-based sensor based on specific CA19-9 antibody

Abstract

The invention discloses a detection method for a paper-based sensor based on a specific CA19-9 antibody. According to the detection method, a filter paper base and carboxylated carbon nanotubes are adopted; the plurality of the carboxylated carbon nanotubes are deposited on the filter paper base; and the carboxylated carbon nanotubes are specifically bound with a specific CA19-9 antibody of pancreatic cancer cells. The detection method of the invention can convert antigen concentration information into directly measurable electrical signals, has the advantages of low manufacturing cost, high precision and high stability, and can realize economical and rapid cancer antigen screening; the sensor of the invention has good detection performance under a low CA19-9 concentration range (from 0 to40U / ml), can detect pancreatic cancer in an early stage; and the detection time of the sensor of the invention only takes about two hours, and the detection efficiency of the sensor is about 12 timeshigher than that of the ELISA (enzyme-linked immune sorbent assay).

Description

technical field [0001] The invention belongs to the field of biosensor technology and cancer detection technology, and in particular relates to a detection method of a paper-based sensor based on a specific CA19-9 antibody. Background technique [0002] Early detection of cancer is crucial for its early detection and early treatment, which can significantly improve the survival chance of patients. Taking pancreatic cancer as an example, it is a malignant tumor of the digestive tract with a high degree of malignancy and is difficult to diagnose and treat. About 90% of them are ductal adenocarcinomas originating from the ductal epithelium. rise. The 5-year survival rate of pancreatic cancer is less than 1%, which is one of the malignant tumors with the highest mortality rate. Early detection of pancreatic cancer is crucial to ensuring high survival rates. Treatment of pancreatic cancer after early detection can extend a patient's five-year survival time by almost 100%, but i...

AI Technical Summary

Problems solved by technology

Enzyme-linked immunosorbent assay (ELISA) is a commonly used method for the detection of CA19-9 in hospitals. This method has poor repeatability, and is prone to interference from autoantibodies and heterophilic antibodies, and is prone to false positives. In addition, using this method However, regardless of instrument and manual operation, there are many interference factors and high cost, so it is not easy to popularize

[0003] Therefore, although there are many early detection methods for cancer and its antigens, they are greatly constrained by the detection limit, detection cost, and detection conditions. Even the relatively mature ELISA detection method is still time-consuming (about 1 day) and expensive. High, and detection of defects requires a well-equipped laboratory

In summary, the existing detection technology is limited by the detection site and conditions, and its high cost will also put greater economic pressure on the subjects

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