3D printing method for cell tissue by using bio-ink

A 3D printing, bio-ink technology, used in the field of biomedicine

Pending Publication Date: 2019-06-18
INST OF APPLIED PHYSICS JIANGXI ACADEMY OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, so far, there is no research report on the use of methylcellulose-based hydrogel 3D printing for cell sheet fabrication.

Method used

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  • 3D printing method for cell tissue by using bio-ink
  • 3D printing method for cell tissue by using bio-ink
  • 3D printing method for cell tissue by using bio-ink

Examples

Experimental program
Comparison scheme
Effect test

Embodiment Construction

[0012] Preparation of methylcellulose hydrogel

[0013] Briefly, methylcellulose hydrogels containing 8% w / v were obtained by mixing methylcellulose powder (cellulose A4M, ŋ = 4000 mPa, 2% in water at 20 °C, supplied by Dow Chemical Company) into Na2SO4 , 0.05 M saline solution. The resulting polymer suspension was stored in a refrigerator at 4 °C for 24 hours to fully hydrate the methylcellulose-based polymer chains. After repeated testing and demonstration, we confirmed that the methylcellulose-based hydrogel formulation has an optimal transition temperature range (LCST=34-37°C) in cell plate engineering and cell delivery applications.

[0014] 3D printer for methylcellulose hydrogel

[0015] Methylcellulose hydrogels are divided into block form and 3D printing form, and their respective preparation methods are shown in Table 1:

[0016] Table 1 Preparation method of methylcellulose-based hydrogel

[0017] name

Methylcellulose-based hydrogel concentration (%...

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PUM

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Abstract

The invention discloses a 3D printing method for MC (methyl cellulose) based hydrogel bio-ink. MC hydrogel is the best matrix to obtain regenerative medicine application cell slices. However, a preparation method of the MC hydrogel currently only allows obtain geometric shapes with standardization and simplicity (namely, geometric shapes of container generated by the hydrogel). By use of the MC hydrogel, corresponding cell plate engineering and 3D printing of complicated shapes of biological tissues and organs can be realized through the 3D printing method. The stability and the rheological property of the method is suitable for an application of biological plate engineering under 37 DEG C. The cells are embedded in the MC based hydrogel, the cell viability is detected to be larger than 90%; the bio-imprinting survival rate of C2C12 cells embedded into the MC based hydrogel is higher than 80% by use of optimized printing parameters.

Description

technical field [0001] The present invention relates to the field of 3D printing and molding of biomedicine and cell plate engineering, more specifically, relates to a method of embedding, culturing and proliferating cells, and adopting the method of biological 3D printing to realize the molding of living tissues and organs with complex structures that can be transplanted. method. Background technique [0002] Smart hydrogels change their structure in response to external factors such as temperature or pH changes. It can be divided into heat-responsive and pH-responsive smart hydrogels. In thermoresponsive hydrogels, N-isopropylacrylamide (PNIPAAm) and methylcellulose (MC) can be used for cell sheet engineering applications as an alternative cytoskeletal tissue engineering approach aimed at overcoming some of the traditional Tissue engineering approaches to cytoskeletal shortcomings, including difficulties in obtaining cell density structures and possible inflammatory scaf...

Claims

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Application Information

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IPC IPC(8): A61L27/38A61L27/20A61L27/52B33Y10/00B33Y70/00B33Y80/00
Inventor 付远谌昀姜江
Owner INST OF APPLIED PHYSICS JIANGXI ACADEMY OF SCI
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