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Method for in-vitro preservation of spathiphyllum kochii germplasm resources

A technology of in vitro preservation and white palm, which is applied in the field of in vitro preservation of plant germplasm resources, can solve the problems of frequent transfer of tissue culture preservation, high cost of planting and preservation, and susceptibility to pests and diseases, so as to ensure the excellent characteristics of germplasm , reduce labor and material costs, and save storage space

Active Publication Date: 2019-06-18
三明市农业科学研究院
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Problems solved by technology

[0006] The present invention provides a method for in vitro preservation of germplasm resources of Aphrodisiac A. costly defects

Method used

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  • Method for in-vitro preservation of spathiphyllum kochii germplasm resources
  • Method for in-vitro preservation of spathiphyllum kochii germplasm resources
  • Method for in-vitro preservation of spathiphyllum kochii germplasm resources

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Embodiment Construction

[0027] Specific embodiments of the present invention will be described below with reference to the accompanying drawings.

[0028] A method for in vitro preservation of germplasm resources of Atractylodes chinensis, comprising the following steps:

[0029] 1) Selection and disinfection of explants: use the thick sucking buds of Alba palmata after removing the leaves as explants, rinse them under tap water first, and then disinfect the surface of the explants with 72-75% alcohol for 45-60 seconds , then rinsed once with sterile water, then treated the explants with 0.1% mercuric chloride for 10-12 minutes, rinsed with sterile water for 3 times, and blotted dry with sterile filter paper for later use; Thick sucking buds are explants; and it is best to use 72% alcohol;

[0030] 2) Propagation of test-tube seedlings: Cut the explants treated in step 1 into segmented stem segments and inoculate them into a bud induction medium with a pH of 5.8-6.0 at a temperature of 27-29° C., li...

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Abstract

The invention discloses a method for in-vitro preservation of spathiphyllum kochii germplasm resources. According to the method, thick and strong spathiphyllum kochii offsets of which leaves are removed serve as explants, the explants are washed thoroughly with tap water, disinfected with 72-75% ethyl alcohol for 45-60 s, then washed with sterile water once, treated by 0.1% mercuric chloride and washed with sterile water 3 times, and sterile filter paper absorbs the water thoroughly. The explants are cut into stems with nodes, then the stems are inoculated to a bud induction culture medium tobe cultured for 30 d, and then germinated axillary buds are transferred to a multiplying culture medium for 40 d of expanding propagation to obtain cluster seedlings; the cluster seedlings are cut into single plants, and the single plants are transferred into an in-vitro preservation culture medium for preservation, wherein the in-vitro preservation culture medium is prepared from 3g / L of hyponexNO.1, 1-3 g / L of NH4NO3, 0.5-1.0 g / L of Centrum adult male and female comprehensive mineral substance decavitamin tablets, 30g / L of saccharose and 4g / L of agar powder. According to the method, spathiphyllum kochii test-tube plantlets of 16 months are preserved, the offspring botanical character and ISSR amplification spectrum bands have no difference, preservation materials are stable, heritable variation cannot occur, species character degradation and disease infection can be prevented, and the excellent feature of the germplasm is guaranteed.

Description

technical field [0001] The present invention relates to the field of in vitro preservation of germplasm resources of plants, in particular to a method for in vitro preservation of germplasm resources of Aphragmus japonicus. Background technique [0002] White palm, also known as white crane lily, is a perennial evergreen herbaceous plant of the Araceae family, white crane lily. It has both leaves and flowers. It also has the function of absorbing toxic and harmful gases such as formaldehyde. It is one of the top ten potted flowers in the market. White palm is distributed in tropical America, West Indies and Malay Archipelago. It has been reported that there are 41 native species and more than 100 cultivated varieties. Due to the heterozygous cultivation, the traits of offspring are easy to separate, and the seed life is extremely short, it is difficult to preserve germplasm resources through seeds, and the female parent is mainly preserved through facility cultivation. This ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00A01N3/00
Inventor 周辉明林辉锋莫智龙陈昌铭郭芸玮夏朝水林发壮姚凤琴陈玮婷曹奕鸯
Owner 三明市农业科学研究院
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