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HPLC finger-print establishing method of small flower sabia japonica

A kind of technology of Xiaohua Qingfengteng and fingerprints, which can be applied to measuring devices, instruments, scientific instruments, etc., and can solve problems such as weak quality control research

Active Publication Date: 2019-06-14
贵州中医药大学
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Problems solved by technology

In recent years, with the support of the National Natural Science Foundation of China and the construction of first-class disciplines in Guizhou Province, the author has carried out research on Tenghan and its related plants, including resource investigation, introduction and cultivation, identification of crude drugs, quality of medicinal materials, chemical components, pharmacological effects, etc. In-depth research on various aspects, it is found that Qingfengteng has high development and utilization value, but the research on quality control is still relatively weak

Method used

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  • HPLC finger-print establishing method of small flower sabia japonica
  • HPLC finger-print establishing method of small flower sabia japonica
  • HPLC finger-print establishing method of small flower sabia japonica

Examples

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Embodiment

[0104] A kind of method for establishing the HPLC fingerprint of genus floridae, comprising the following steps:

[0105] (1) Preparation of mixed reference solution: Accurately weigh quercetin-3-O-gentiobioside, Camellianoside, Tsubakioside A, kaempferol-3-O-rutinoside, isorhamnetin-3-O - Rutinoside reference substance, add 70% ethanol solution to dissolve, shake well, and make quercetin-3-O-gentiobioside with a mass concentration of 0.0625mg / mL, Camellianoside with a mass concentration of 0.250mg / mL, and Tsubakioside A with a mass concentration A mixed reference solution with a concentration of 0.0675 mg / mL, a mass concentration of kaempferol-3-O-rutinoside of 0.0625 mg / mL, and a mass concentration of isorhamnetin-3-O-rutinoside of 0.0500 mg / mL;

[0106] (2) Preparation of the test solution: Accurately weigh 1g of the powder of Fructus japonica, put it in a 50mL conical flask with a stopper, add 15mL of 70% ethanol solution accurately, weigh it, under the conditions of 100W ...

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Abstract

The invention discloses an HPLC finger-print establishing method of a small flower sabia japonica. The HPLC finger-print establishing method of the small flower sabia japonica has the advantages of convenience, stability, high precision and great repeatability; the kind and number of chemical components contained in the small flower sabia japonica can further be reflected more thoroughly by adopting the HPLC finger-print establishing method, and thus the overall description and evaluation of the quality of the small flower sabia japonica are further conducted. Detected small flower sabia japonica finger-print by using the HPLC finger-print establishing method includes more chemical components, the height proportion of all characteristic peaks are moderate, the base line is more stable, thedegree of separation and the peak shape are great, and the column efficiency is high.

Description

technical field [0001] The invention relates to a method for establishing an HPLC fingerprint of Qingfengteng chrysalis, belonging to the field of pharmaceutical technology. Background technique [0002] Traditional Chinese medicine fingerprint is a comprehensive and quantifiable means of identification. It is based on the systematic research on the chemical components of traditional Chinese medicine and is mainly used to evaluate the authenticity, goodness and stability of the quality of Chinese medicinal materials and semi-finished products of traditional Chinese medicine preparations. Traditional Chinese medicine and its preparations are multi-component complex systems, so the evaluation of its quality should adopt a detection method that is suitable for it and can provide rich identification information. The type and quantity of chemical components, and then the overall description and evaluation of drug quality. On this basis, if the study of spectrum efficacy is furth...

Claims

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Application Information

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IPC IPC(8): G01N30/06G01N30/74G01N30/86
Inventor 徐文芬孙庆文潘国吉王波陈胤睿
Owner 贵州中医药大学
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