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Marker for auxiliary detection of wheat high SNP (Single Nucleotide Polymorphism) and application thereof

An auxiliary detection and spikelet number technology, which is applied in the field of wheat genetics and breeding, can solve the problems of poor stability, heavy workload, and low efficiency, and achieve the effects of stable labeling, easy operation, and mature and stable technology

Active Publication Date: 2019-05-28
CHENGDU INST OF BIOLOGY CHINESE ACAD OF S
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  • Abstract
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Problems solved by technology

[0004] The purpose of the present invention is to provide a marker and its application for auxiliary detection of wheat high spikelet number traits, which solves the problems of large workload, long cycle, low efficiency and poor stability in conventional breeding methods

Method used

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  • Marker for auxiliary detection of wheat high SNP (Single Nucleotide Polymorphism) and application thereof
  • Marker for auxiliary detection of wheat high SNP (Single Nucleotide Polymorphism) and application thereof
  • Marker for auxiliary detection of wheat high SNP (Single Nucleotide Polymorphism) and application thereof

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Embodiment Construction

[0027] The present invention will be described in detail below in combination with specific embodiments.

[0028] 1. Using CTAB method to extract wheat genomic DNA, extraction steps:

[0029] (1) Take 100 mg of fresh young leaves, grind them into fine powder with liquid nitrogen, add 2×CTAB extract (2% CTAB; 1.4M NaCl, 0.1MTris-HCl, PH8.0, 0.1M EDTA) preheated to 65°C ,PH8.0) 1ml, mix well;

[0030] (2) 65 ℃ water bath for 30-45min, during which shake gently to mix. After cooling to room temperature, add an equal volume of chloroform:isoamyl alcohol (24:1), mix gently until the supernatant becomes milky, and centrifuge at 4000rpm for 10min;

[0031] (3) Take the supernatant, add an equal volume of isopropanol, and place in an ice bath to precipitate DNA;

[0032] (4) Centrifuge at 4000rpm for 10min, remove the supernatant, wash twice with 70% ethanol and once with absolute ethanol. Air-dry the DNA and dissolve it in an appropriate amount of 1×TE solution with pH 8.0. Add R...

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Abstract

The invention aims at providing a marker for auxiliary detection of wheat high SNP (Single Nucleotide Polymorphism) and application thereof. The SNP marker is positioned on 553937825-th site nucleotide on a 3D chromosome of a hexaploidy ordinary wheat gene; the basic group of the SNP marker in a high-single-spike spikelet number wheat material is G; the basic group of the SNP marker in a low-single-spike spikelet number wheat material is A. The marker has the beneficial effects that the marker is a colinear marker; through digestion belt type, the typing can be accurately performed; through the typing detection on 164 individuals, the accurate rate reaches 85 percent.

Description

technical field [0001] The invention belongs to the technical field of wheat genetics and breeding, and relates to a SNP (Single Nucleotide Polymorphism) marker for auxiliary detection of the trait of high spikelet number per spike in wheat and an application thereof. Background technique [0002] Wheat belongs to Gramineae, Triticeae, and Triticum. It is one of the most important and widely cultivated food crops in the world, providing important energy intake for about 35% of the world's population. Intake and source of protein. However, with population growth, climate change, and reduction of available arable land, the food crisis is becoming more and more serious. Therefore, the high and stable yield of wheat has always been the research focus of countries all over the world. The number of spikes per unit area, the number of grains per spike, and the thousand-grain weight are the three major elements of wheat yield. Therefore, the number of grains per panicle is closely...

Claims

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Application Information

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IPC IPC(8): C12Q1/6895C12N15/11
Inventor 龙海李涛邓光兵张海莉梁俊俊余懋群
Owner CHENGDU INST OF BIOLOGY CHINESE ACAD OF S
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