Method for preparing sapogenin through chenopodium quinoa willdL seed coat and separation and quantification method of sapogenin

A technology for quinoa saponin and sapogenin, which is applied in the field of preparation of sapogenin and its separation and characterization, can solve the problems of quinoa nutritional value, active substances and less research on its application, etc., so as to improve comprehensive utilization rate and economic added value, Conducive to the reuse of resources and the effect of sustainable development

Inactive Publication Date: 2019-05-28
QINGHAI NORMAL UNIV
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  • Abstract
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Problems solved by technology

Although quinoa has been introduced all over the world, the current research on quinoa is mostly in the stage of breeding, planting and primary processing, and there are few studies on the nutritional value, active substances and applications of quinoa

Method used

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  • Method for preparing sapogenin through chenopodium quinoa willdL seed coat and separation and quantification method of sapogenin
  • Method for preparing sapogenin through chenopodium quinoa willdL seed coat and separation and quantification method of sapogenin

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Embodiment 1

[0043] Materials and reagents used in this example include: oleanolic acid standard, hedera saponin standard, Shanghai Yuanye Biotechnology Co., Ltd. (content ≥ 98 (HPLC)); chromatographic grade methanol, American MREDA company.

[0044] The instruments and equipment used in this embodiment include: QTRAP 6500+LCMS system (triple quadrupole-ion trap mass spectrometer), AB SCIEX, USA.

[0045] (1) Separation and extraction of quinoa saponins

[0046] Accurately weigh 2g of quinoa seed coat powder in a 500mL round bottom flask, add 30mL of deionized water and a compound enzyme preparation total amount of 1.5% (0.03g), enzyme ratio [m (cellulase): m (pectin Enzyme)] was 3:2, the temperature of the adjustment system was 50.5°C, the pH value was 5.5, and the enzyme was hydrolyzed with electromagnetic stirring for 17 minutes; after the enzymolysis was completed, the enzyme was inactivated at 90°C for 30 seconds; 70 mL of absolute ethanol was added, and ultrasonically extracted for 2...

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Abstract

The invention provides a method for preparing sapogenin through chenopodium quinoa willdL seed coat and a separation and quantification method of the sapogenin, and belongs to the technical field of biological product processing. The method for preparing the sapogenin through the chenopodium quinoa willdL seed coat comprises the following steps that firstly, the chenopodium quinoa willdL seed coat, complex enzyme and deionized water are mixed and enzymolyzed to obtain enzymatic hydrolysate; then the enzymatic hydrolysate and ethyl alcohol are mixed, chenopodium quinoa willdL saponin in the enzymatic hydrolysate is extracted through ultrasonic waves, solid-liquid separation is conducted, and a chenopodium quinoa willdL saponin extracting solution is obtained; then the chenopodium quinoa willdL saponin extracting solution and hydrochloric acid are mixed and subjected to acidolysis to obtain acidolysis liquid; and finally, the acidolysis liquid is extracted through petroleum ether to obtain extract liquor, and a solvent in the extract liquor is removed through evaporation, and the sapogenin is obtained. According to the method, the chenopodium quinoa willdL seed coat serves as a raw material to prepare the sapogenin, the natural sapogenin raw material can be provided for a professional pharmaceutical factory, the comprehensive utilization rate and the economic added value of chenopodium quinoa willdL can further be increased, and reuse and sustainable development of resources are facilitated. The invention further provides a method for simultaneous separation and quantification of oleanolic acid and hederagenin in the chenopodium quinoa willdL seed coat through UPLC-MS.

Description

technical field [0001] The invention belongs to the technical field of biological product processing, and in particular relates to a method for preparing sapogenin from quinoa seed coat and its separation and characterization. Background technique [0002] Quinoa (Chenopodium quinoa WilldL.), also known as South American quinoa, quinoa, etc., belongs to Chenopodiaceae. It is a dicotyledonous plant that originated in the Andes region of South America. It is rich in vitamins and trace elements (such as minerals potassium, iron, calcium, etc.) ), dietary fiber, protein and essential amino acids, and contains saponins, polyphenols, flavonoids, phytosterols and other bioactive ingredients, which have antifungal, antiviral, anticancer, lowering cholesterol, lowering blood sugar, antithrombotic, diuretic, anti It is known as "the mother of food" and is defined as "one of the food in the 21st century". Although quinoa has been introduced all over the world, the current research on ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07J63/00G01N30/02
Inventor 张炜陈元涛雷蕾于小栋王志娟辛小丽田格甘文梅
Owner QINGHAI NORMAL UNIV
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