Application of scillaren compound to preparation of flavivirus infection resistant medicament

A kind of compound, the technology of onionin, applied in the field of medicine, can solve the problem of not found anti-flavivirus and other problems

Inactive Publication Date: 2019-05-28
INST OF MATERIA MEDICA AN INST OF THE CHINESE ACAD OF MEDICAL SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

No reports of anti-flavivirus (including Zika virus, dengue virus, yellow fever virus, Japane

Method used

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  • Application of scillaren compound to preparation of flavivirus infection resistant medicament
  • Application of scillaren compound to preparation of flavivirus infection resistant medicament
  • Application of scillaren compound to preparation of flavivirus infection resistant medicament

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0047] Embodiment 1. compound anti-flavivirus activity detection method

[0048] 1.1. Preparation of the test compound solution: the test compound was dissolved in DMSO as a solvent, the final concentration of the DMSO medium was 0.1%, and the final concentration of the test compound: 100nM, 30nM, 10nM, 3nM, 1nM.

[0049] 1.2. Anti-Zika virus activity detection method of compounds

[0050] The day before infection, the cells were inoculated with 2.0×10 4The density per well was inoculated into a 96-well plate, and the test compound was added, with DMSO as the control. After 1 h, the original culture medium was discarded, and Zika virus (GZ01 / 2016 strain, FSS13025 / 2010 strain and SZ01 / 2016 strain) culture medium (MOI: 0.5) was added, and the test compound was added at the same time. 24 hours after infection, the virus liquid was discarded, the cells were fixed with 4% paraformaldehyde solution, and PBS containing 0.1% Triton X-100 was added, and PBS containing 1% goat serum a...

Embodiment 2

[0077] Example 2. Insaridin A anti-flavivirus activity (Table 5)

[0078] 2.1. Anti-Zika virus activity of insaridin A

[0079]

[0080] The experimental results showed that insaridin A has anti-Zika virus activity. In this experiment, Zika virus GZ01 / 2016 strain and two other Zika virus strains (FSS13025 / 2010, SZ01 / 2016) were used, and it was found that instriside A had an inhibitory effect on them, and the half inhibitory concentration (IC 50 ) were 5.6nM, 4.9nM, 4.7nM, respectively.

[0081] 2.2. Compound anti-dengue virus activity

[0082] The experimental results showed that instritin A had anti-dengue virus activity, and the half maximal inhibitory concentration (IC 50 ) is 3.3nM.

[0083] 2.3. Compound anti-yellow fever virus activity

[0084] The experimental results showed that instritin A had anti-yellow fever virus activity, and the half inhibitory concentration (IC 50 ) is 3.4nM.

[0085] 2.4. Anti-Japanese Encephalitis Virus Activity of Compounds

[008...

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Abstract

The invention discloses application of a scillaren compound to preparation of a flavivirus infection resistant medicament, including application of the scillaren compound to the prevention or treatment of flavivirus infection or combined application with other antiviral medicaments. The structural formula of the scillaren compound is shown in the description as a formula (I) , wherein R is selected from monosaccharide and oligosaccharide; the monosaccharide includes rhamnose, 6-deoxysugar, quinovose, digitoxose, glucose, xylose and 4,6-deoxyazolone; the oligosaccharide includes oligosaccharideconsisting of the monosaccharide.

Description

technical field [0001] The invention relates to the application of calicoside compounds in anti-flavivirus infection, and belongs to the technical field of medicine. The present invention includes single or combined application of calicoside compounds in anti-flavivirus prevention or treatment. Background technique [0002] Flavivirus (Flavivirus) belongs to Flaviviridae (Flaviviridae), this family has three virus genera, namely Flavivirus (Flavivirus), Pestivirus (Pestivirus) and Hepacivirus (Hepacivirus) [Ming GL, TangH, Song H. Advances in Zika Virus Research: Stem Cell Models, Challenges, and Opportunities. J. Cell stem cell 2016; 19(6), 690-702.]. [0003] Flavivirus is a single-strand negative-strand RNA virus. The viral genome encodes 10 proteins, of which 3 are structural proteins: capsid protein (Capsid protein, C), immature membrane protein (Premembrane protein, prM), envelope protein ( Envelope protein, E); 7 non-structural proteins (NS1, NS2A, NS2B, NS3, NS4A, ...

Claims

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Application Information

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IPC IPC(8): A61K31/7048A61P31/14
CPCY02A50/30
Inventor 郭颖覃晓玲唐克陈勍徐燕
Owner INST OF MATERIA MEDICA AN INST OF THE CHINESE ACAD OF MEDICAL SCI
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