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Application of Streptococcus salivarius k12 in the preparation of medicaments for preventing and/or treating autoimmune diseases

A technology of Streptococcus salivarius and K12, which is applied in the field of treatment of autoimmune diseases, can solve problems such as concomitant infection, and achieve the effects of regulating immune balance, reducing the incidence of disease, and alleviating swelling symptoms.

Active Publication Date: 2020-11-17
PEOPLES HOSPITAL PEKING UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, excessive use of hormones, immunosuppressants, and biological agents to suppress the immune response cannot achieve the purpose of effectively alleviating the disease, and is usually accompanied by adverse reactions such as infection

Method used

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  • Application of Streptococcus salivarius k12 in the preparation of medicaments for preventing and/or treating autoimmune diseases
  • Application of Streptococcus salivarius k12 in the preparation of medicaments for preventing and/or treating autoimmune diseases
  • Application of Streptococcus salivarius k12 in the preparation of medicaments for preventing and/or treating autoimmune diseases

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0026] Streptococcus salivarius significantly reduced in the tonsil microbiota of patients with rheumatoid arthritis

Embodiment approach

[0028] (1) Collection of tonsil flora samples from patients with rheumatoid arthritis RA and healthy subjects: 1) Instruct the subjects to rinse their mouth gently and avoid touching the throat; 2) Use a moist sterile cotton swab to dip Take the surface of the tonsils of the subjects, and pay attention to avoid touching the saliva, the back of the tongue, the palate, the inner wall of the oral cavity and other parts to prevent bacterial contamination; 3) The bacterial flora samples are temporarily stored in a -80°C low-temperature refrigerator for future use.

[0029] (2) Next-generation sequencing analysis of tonsillar flora: 1) use DNA extraction kit (Catalog No. 12888-100, MO BIO Laboratories, Carlsbad, CA 92010, USA) to extract the genomic DNA of all flora in the sample; 2) use The Illumina library construction kit was used for library construction, and the quality inspection was carried out in strict accordance with the library quality inspection plan recommended by Illumi...

Embodiment 2

[0039] Streptococcus salivarius inhibits the development of experimental arthritis

[0040] method of execution

[0041] (1) Construction of experimental arthritis (CIA) mouse model: 1) Dissolving collagen, dissolving bovine type II collagen (CII) in 0.1mol glacial acetic acid, adjusting the final concentration of CII to 4mg / mL, and overnight at 4°C.

[0042] 2) Emulsifying collagen, mixing completely dissolved CII with an equal volume of Freund's complete adjuvant (CFA) to fully emulsify the two to prepare a collagen emulsion with a final concentration of 2 mg / mL.

[0043] 3) For the first immunization, DBA / 1 mice (purchased from Beijing Huafukang Biotechnology Co., Ltd., male, 6-8 weeks old) were depilated at the base of their tails, and a total of 200 μg of collagen emulsion was injected intradermally at multiple points.

[0044] 4) Booster immunization. On the 21st day after the initial immunization, dissolve type II collagen in 0.1mol glacial acetic acid (concentration 4...

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Abstract

The invention provides an application of streptococcus salivarius K12 in preparing a medicament for preventing and / or treating autoimmune diseases, and belongs to the technical field of treatment of autoimmune diseases. Preventive intervention of the streptococcus salivarius K12 can inhibit occurrence and development of experimental arthritis, so that the incidence of the experimental arthritis isreduced by 30-70% and the synovial tissue of a joint can be prevented from infiltration and erosion of inflammatory cells; in addition, streptococcus salivarius also has therapeutic effects on the experimental arthritis. The streptococcus salivarius K12 can significantly down-regulate the ratio of Tfh cells, GCB cells, DC cells and Neu cells, inhibit the expression of proinflammatory cytokine IL-6, and promote the secretion of anti-inflammatory cytokine IL-10. The streptococcus salivarius can promote the immune steady-state reconstruction by inhibiting the immune response and provides a potential target for the treatment of rheumatoid arthritis; can target and regulate abnormal immune cells, treat diseases from an upstream way, and regulate immune balance.

Description

technical field [0001] The invention belongs to the technical field of autoimmune diseases, and in particular relates to the application of Streptococcus salivarius K12 in the preparation of medicines for preventing and / or treating autoimmune diseases. Background technique [0002] Autoimmune diseases, such as rheumatoid arthritis (RA), systemic lupus erythematosus (SLE) and Sjogren's syndrome (SS), are characterized by immune inflammation, and a variety of pathogenic autoantibodies and multiple pathogenic autoantibodies appear in serum. Systemic involvement is the main clinical feature of this disease. The lesions of rheumatoid arthritis mainly invade the joints, and the symptoms of the joints generally recur, and the damage of the joints becomes more and more serious, eventually leading to varying degrees of dysfunction and deformity. In addition to joints, skin rheumatoid nodules, arteritis, pericarditis, scleritis, lymphadenitis, hepatosplenomegaly, and neuropathy are n...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K35/744A61P29/00A61P19/02A61P37/02A61P37/06
Inventor 栗占国李静何菁靳家扬覃俊杰李胜辉
Owner PEOPLES HOSPITAL PEKING UNIV
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