Compound biopesticide for preventing and treating apple canker and preparation method thereof

A technology of biological pesticides and microbial strains, applied in botany equipment and methods, chemicals for biological control, biocides, etc., can solve the problems of drug resistance of pathogenic bacteria, serious threats to human health, environmental pollution, etc., to achieve Strong permeability, improved disease resistance of crops, and convenient use

Inactive Publication Date: 2019-05-21
SHENYANG AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In addition, there are reports on the use of double-volume Bordeaux mixture (Cu: CaO: H2O = 1:2:200) to prevent and control apple tree rot, which has a high inhibition rate on the germination of conidia of the apple tree rot fungus, but its Disclosed are chemical pesticides used to control hazelnut powdery mildew
[0004] In addition, due to the extensive and long-term use of chemical agents, it not only poses a serious threat to human health, but also often causes environmental pollution and drug resistance of pathogenic bacteria. Key problems that need to be solved urgently in production

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0032] Example 1 Preparation of compound biological pesticide for preventing and controlling apple tree rot

[0033] The pesticide includes a microbial strain aqua part and a Chinese herbal medicine aqua part, and the microbial strain aqua part includes Bacillus amyloliquefaciens, Paecilomyces lilacinus (Thom.) Samson and Harzia Mould (Trichoderma harzianum); the Chinese herbal medicine liquid part includes garlic extract, spatholobi extract, perilla extract and sophora flavescens extract. Wherein, the preparation method of the microbial strain liquid part includes the following steps:

[0034] (1) Put Paecilomyces lilacinus and Trichoderma harzianum in a PD (potato) liquid medium at 25°C for 3 days, and Bacillus amyloliquefaciens in a beef extract peptone medium at 25°C for 3 days. The three kinds of bacteria were centrifuged and freeze-dried to make a live bacteria preparation,

[0035] (2) Mix the living bacteria preparations of the above three kinds of bacteria, and then add th...

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PUM

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Abstract

The embodiment of the invention discloses compound biopesticide for preventing and treating apple canker. The biopesticide comprises an aqueous microbial strain agent part and an aqueous traditional Chinese medicine agent part, the aqueous microbial strain agent part comprises bacillus amyloliquefaciens, paecilomyces lilacinus and trichoderma harzianum; the aqueous traditional Chinese medicine agent part comprises a garlic extract, a suberect spatholobus stem extract, a purple perilla extract and a radix sophorae flavescentis extract. The biopesticide can induce apple trees to generate resistance, improves the fertilizer efficiency of soil, achieves the sterilization effect, and is novel biopesticide with fertilizer and pesticide effects. The embodiment of the invention further provides apreparation method for the compound biopesticide.

Description

Technical field [0001] The invention relates to the technical field of biological pesticides, in particular to a compound biological pesticide for preventing and curing apple tree rot and a preparation method thereof. Background technique [0002] Apple tree rot disease (Valsa mali Miyabe et Yamada), also known as stinky skin disease and rot disease, is a type of fungal disease caused by the fungi of the genus Black rot. It has the characteristics of common occurrence, serious damage and difficult to cure. Important limiting factors for apple yield and quality. In the event of a rot disease in an apple orchard, the lesser ones will reduce the production of apples, the more severe ones will cause a large number of fruit trees to die or even destroy the orchard, which will cause serious economic losses to the fruit farmers. Apple tree rot disease has become an important disease threatening the development of my country's apple industry. [0003] At present, chemical agents are comm...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01N65/42A01N63/04A01N63/00A01P3/00
CPCY02A50/30
Inventor 何璐朱赫王凯李兴海刘睿博慈峻哲
Owner SHENYANG AGRI UNIV
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