Bifurcated vessel model and preparation method thereof

Abstract

The invention provides a bifurcated vessel model and a preparation method thereof. The bifurcated vessel model comprises a main vessel line and at least one branch vessel line. Materials of the bifurcated vessel model comprise biohydrogel, proliferous smooth muscle cells or smooth muscle cell precursor cells which can differentiate into the smooth muscle cells and mesenchymal stem cells. The content of the smooth muscle cells or the smooth muscle cell precursor cells is 1*10^6-10^7 cells per gram of biohydrogel, and the content of the mesenchymal stem cells is 1*10^5-5*10^6 cells per gram of biohydrogel. The vessel inner wall surface comprises a proliferous endothelium layer. The bifurcated vessel has good vascular function characteristics. A biological model and a disease model established by the bifurcated vessel can further satisfy the pathological research of diseases and the need for in vitro screening of a therapeutic drug.

Description

【Technical field】 [0001] The invention belongs to the field of artificial organs and organ chips in tissue engineering, and in particular relates to a model with bifurcated blood vessels and a preparation method thereof. 【Background technique】 [0002] The artificial blood vessels currently on the market are generally made of chemical materials such as synthetic polymers (such as ePTEF materials, polyethyleneterephthalatefibre). The artificial blood vessel models made of these materials are usually used to replace a small amount of blood vessels in the patient's body, usually larger Blood vessels (over 6mm in diameter). However, these artificial blood vessels are made of chemical materials, so the artificial blood vessels do not contain cells and living substances, so they have little biological functionality and are only used as a passage for a section of blood vessels. [0003] Existing biomaterials are used to prepare artificial blood vessels using a biomaterial stent me...

AI Technical Summary

Problems solved by technology

However, in the process of preparing the artificial blood vessel model, due to the special use of the artificial blood vessel model in biomedicine, the requirements for the size, temperature, non-toxicity, and purity of the prepared material are very strict, and due to the fragility of the artificial blood vessel model itself Due to strict requirements on preparation, installation, and disassembly, no further breakthroughs have been made in the preparation and development of artificial vascular models, and complex vascular structures cannot be produced, which cannot meet the requirements for further improvement of biological models and disease models in the field of tissue engineering.

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