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Method for quantitative determination of catechins

A technology for the quantitative determination of catechins, applied in the direction of material excitation analysis, fluorescence/phosphorescence, etc., can solve the problems of high cost, cumbersome operation, time-consuming, etc., and achieve the effect of good stability and high sensitivity

Active Publication Date: 2019-05-10
SOUTH CENTRAL UNIVERSITY FOR NATIONALITIES
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Although these methods have high precision, high sensitivity, and low cross-interference, they require complex derivatization steps, a large amount of organic solvents are required in the experimental process, the operation is cumbersome, time-consuming, expensive, and inconvenient for on-site detection

Method used

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  • Method for quantitative determination of catechins
  • Method for quantitative determination of catechins
  • Method for quantitative determination of catechins

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0044] Embodiment 1: The recognition and quantitative analysis of catechin by the reversible nano-porphyrin fluorescent sensor, the schematic diagram of the method is shown in 1, and the steps are as follows:

[0045] (1) Synthesis of ZnCdSe quantum dot fluorescent probe

[0046] Dissolve zinc dichloride (0.035g, 6.4mM) and N-acetyl-L-cysteine ​​(0.1253g, 19.2mM) in 40mL of ultrapure water, stir for 20 minutes in an ice bath, and use Adjust the pH of the solution to 9.7 with sodium hydroxide solution, adjust the pH and add 100 μL of cadmium dichloride (0.00058 g, 0.237 mM), then fill with nitrogen and stir in an ice bath for 5 to 10 minutes. NaHSe was added and stirred for 5 minutes. Finally, the solution was put into a reaction kettle and reacted in an oven at 200° C. for 65 minutes. Cooling to room temperature yielded 4.9×10 -9 mol / LZnCdSe quantum dot fluorescent probe.

[0047] (2) Synthesis of nanoporphyrin solution

[0048] Dissolve an appropriate amount of tetrakis-...

Embodiment 2

[0054] Embodiment 2: Quantitative analysis of epicatechin by the reversible nano-porphyrin fluorescent sensor, the schematic diagram of the method is shown in 1, and the steps are as follows:

[0055] (1) Synthesis of ZnCdSe quantum dot fluorescent probe

[0056] The ZnCdSe quantum dot fluorescent probe was synthesized by the method of step (1) in Example 1.

[0057] (2) Synthesis of tetrakis-(4-pyridyl)zinc porphyrin self-assembly solution

[0058] The method of step (2) in Example 1 was used to synthesize tetrakis-(4-pyridyl)zinc porphyrin self-assembly solution.

[0059] (3) Preparation of switch nanoporphyrin fluorescent sensor

[0060] The nano-porphyrin fluorescence sensor was prepared by the method of step (3) in Example 1.

[0061] (4) Quantitative analysis of epicatechin by reversible nano-porphyrin fluorescent sensor

[0062] Add 100 μL epicatechin aqueous solution to 1.5mL cuvette, 300 μL 1.68×10 -5 mol / L tetrakis-(4-pyridyl)zinc porphyrin self-assembly solution ...

Embodiment 3

[0064] Example 3: Quantitative analysis of epigallocatechin gallate by a reversible nanoporphyrin fluorescent sensor, the schematic diagram of the method is shown in Figure 1, and the steps are as follows:

[0065] (1) Synthesis of ZnCdSe quantum dot fluorescent probe

[0066] The ZnCdSe quantum dot fluorescent probe was synthesized by the method of step (1) in Example 1.

[0067] (2) Synthesis of tetrakis-(4-pyridyl)zinc porphyrin self-assembly solution

[0068] The method of step (2) in Example 1 was used to synthesize tetrakis-(4-pyridyl)zinc porphyrin self-assembly solution.

[0069] (3) Preparation of switch nanoporphyrin fluorescent sensor

[0070] The nano-porphyrin fluorescence sensor was prepared by the method of step (3) in Example 1.

[0071] (4) Quantitative analysis of epigallocatechin gallate by a reversible nanoporphyrin fluorescent sensor

[0072] Add 100μL epigallocatechin gallate aqueous solution to 1.5mL cuvette, 300μL 1.68×10 - 5 mol / L tetrakis-(4-pyr...

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Abstract

The invention relates to a method for quantitative determination of catechins, and belongs to the technical field of nanomaterialpreparation and chemical analysis detection. The method for the quantitative determination of the catechins comprises the steps that a four-(4-pyridyl) zinc porphyrinphotosensitive effect of self-assembled nanocrystallization and ZnCdSe quantum dots are used for preparing and obtaining a reversible nanometer porphyrin fluorescence sensor with a nanometer effect in a buffle sollution system; and then strong acting force between the catechins and the nanometer porphyrin is used for opening up different degrees of specific changes of the reversible composite sensing interface between the nanometer porphyrin and the quantum dots, so that the quantitative detection ofthe catechins is realized. The method for the quantitative determination of the catechins is simple and controllable, has high sensitivity, good selectivity and high specificity of the detection method of the catechins, and can play an important role in the detection of the catechins.

Description

technical field [0001] The invention belongs to the technical field of nanomaterial preparation and content determination, and in particular relates to a controllable preparation of a novel reversible nanoporphyrin fluorescence sensor and a method for highly sensitive detection of catechins. Background technique [0002] The detection and quantification of catechins is important for a healthy diet due to their many beneficial effects and uses. At present, a variety of detection methods have been used for the detection and quantification of catechins. Detection methods include: electrochemical high performance liquid chromatography, capillary electrophoresis, electrospray mass spectrometry, high-speed countercurrent chromatography, diode array, etc. Although these methods have high precision, high sensitivity, and low cross-interference, they require complex derivatization steps, a large amount of organic solvents are required in the experimental process, the operation is cu...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N21/64
Inventor 付海燕郭晓明佘远斌时琼杨天鸣
Owner SOUTH CENTRAL UNIVERSITY FOR NATIONALITIES
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