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A method for aluminum ion-induced phosphorescent copper nanocluster aggregation-enhanced fluorescence detection and its application

A copper nano-cluster and fluorescence-enhancing technology, applied in the field of biosensing, can solve the problems of low detection limit, complicated operation, high price, etc., and achieve the effects of fast reaction, simple synthesis and reduced competition effect.

Active Publication Date: 2022-04-05
NANJING TECH UNIV
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

In these methods, there are generally disadvantages such as low detection limit, high price, complicated operation, etc.

Method used

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  • A method for aluminum ion-induced phosphorescent copper nanocluster aggregation-enhanced fluorescence detection and its application
  • A method for aluminum ion-induced phosphorescent copper nanocluster aggregation-enhanced fluorescence detection and its application
  • A method for aluminum ion-induced phosphorescent copper nanocluster aggregation-enhanced fluorescence detection and its application

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0057] Embodiment 1: Get DMF and deionized water configuration 5mL mixed solution in 20mL glass bottle, DMF / H 2 O volume ratio is 2:1. Prepare 0.025M glutathione solution, add 200 μL of glutathione solution to the above mixed solution, shake gently until uniform, and the concentration of GSH in the mixed solution is 1 mM. Then configure 1M copper sulfate solution, take 5 μL of copper sulfate solution into the glass bottle, so that the concentration of copper ions in the mixed solution is 1000 μM. Put the glass bottle into a shaking box at 20°C, stir and shake at a slow speed for 1 minute to form phosphorescent copper nanoclusters. The prepared copper nanoclusters were measured by a fluorescence spectrophotometer to draw a fluorescence curve, such as figure 1 .

Embodiment 2

[0058] Embodiment 2: Get DMF and deionized water configuration 5mL mixed solution in 20mL glass bottle, DMF / H 2 O volume ratio is 2:1. Prepare 0.05M glutathione solution, add 200 μL of glutathione solution to the above mixed solution, shake gently until uniform, and the concentration of GSH in the mixed solution is 2 mM. Then configure 1M copper sulfate solution, take 5 μL of copper sulfate solution into the glass bottle, so that the concentration of copper ions in the mixed solution is 1000 μM. Put the glass bottle into a shaking box at 20°C, stir and shake at a slow speed for 1 minute to form phosphorescent copper nanoclusters. The prepared copper nanoclusters were measured by a fluorescence spectrophotometer to draw a fluorescence curve, such as figure 2 .

Embodiment 3

[0059] Embodiment 3: get DMF and deionized water configuration 5mL mixed solution in 20mL glass bottle, DMF / H 2 O volume ratio is 2:1. Prepare 0.1M glutathione solution, add 200 μL of glutathione solution to the above mixed solution, shake gently until uniform, and the concentration of GSH in the mixed solution is 4 mM. Then configure 1M copper sulfate solution, take 5 μL of copper sulfate solution into the glass bottle, so that the concentration of copper ions in the mixed solution is 1000 μM. Put the glass bottle into a shaking box at 20°C, stir and shake at a slow speed for 1 minute to form phosphorescent copper nanoclusters. The prepared copper nanoclusters were measured by a fluorescence spectrophotometer to draw a fluorescence curve, such as image 3 .

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Abstract

The invention relates to a method for detecting aluminum ions based on aluminum ion-induced phosphorescent copper nano-cluster aggregation enhanced fluorescence and an application thereof, belonging to the technical field of nanobiological sensing. Copper nanoclusters (Cu NCs) with phosphorescent properties were prepared by adding glutathione and copper sulfate to a mixed solvent of DMF and deionized water at room temperature. Aluminum ions (Al 3+ ) can effectively enhance the fluorescence of Cu NCs, while pyrophosphate and aluminum ions have a high affinity so that Cu NCs / Al 3+ The fluorescence of the system decreases. to Cu NCs / Al 3+ A co-incubation solution of alkaline phosphatase and pyrophosphate was added to the system. Since alkaline phosphatase can hydrolyze pyrophosphate, it effectively reduces the effect of pyrophosphate on Cu NCs / Al. 3+ Al in the system 3+ of competition. Quantitative detection of alkaline phosphatase is realized through the change of fluorescence intensity.

Description

technical field [0001] The invention relates to a method for detecting aluminum ions based on aluminum ion-induced aggregation of phosphorescent copper nano-clusters to enhance fluorescence and an application thereof, belonging to the technical field of biosensing. Background technique [0002] Metal nanoclusters are composed of several to dozens of metal atoms, and the size is close to the electron Fermi wavelength. It is a new type of fluorescent nanomaterial. Due to the ultra-small size of metal nanoclusters, it has excellent size effect, excellent photophysical properties and catalytic effect. From biological probes to catalysts, LEDs to photovoltaic cells, metal clusters have been widely studied and applied in many fields. Among them, metal copper nanoclusters have higher reactivity and higher correlation with molecular proteins and enzymes. In recent years, metal nanoclusters have attracted widespread attention due to their unique chemical and physical properties, an...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N21/64B82Y40/00B82Y15/00B22F9/24B22F1/00
Inventor 刘金华慈乔乔常进张承武李林张倩晨邹昌鹏
Owner NANJING TECH UNIV
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