Method for inspecting aluminium ion based on aluminium ion induced phosphorescent copper nano-cluster gathering enhanced fluorescence and application of method
A technology of copper nano-clusters and enhanced fluorescence, applied in the field of biosensing, can solve the problems of low detection limit, high price, complicated operation, etc., and achieve the effects of fast response, reduced competition, and simple synthesis
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Embodiment 1
[0057] Embodiment 1: Get DMF and deionized water configuration 5mL mixed solution in 20mL glass bottle, DMF / H 2 O volume ratio is 2:1. Prepare 0.025M glutathione solution, add 200 μL of glutathione solution to the above mixed solution, shake gently until uniform, and the concentration of GSH in the mixed solution is 1 mM. Then configure 1M copper sulfate solution, take 5 μL of copper sulfate solution into the glass bottle, so that the concentration of copper ions in the mixed solution is 1000 μM. Put the glass bottle into a shaking box at 20°C, stir and shake at a slow speed for 1 minute to form phosphorescent copper nanoclusters. The prepared copper nanoclusters were measured by a fluorescence spectrophotometer to draw a fluorescence curve, such as figure 1 .
Embodiment 2
[0058] Embodiment 2: Get DMF and deionized water configuration 5mL mixed solution in 20mL glass bottle, DMF / H 2 O volume ratio is 2:1. Prepare 0.05M glutathione solution, add 200 μL of glutathione solution to the above mixed solution, shake gently until uniform, and the concentration of GSH in the mixed solution is 2 mM. Then configure 1M copper sulfate solution, take 5 μL of copper sulfate solution into the glass bottle, so that the concentration of copper ions in the mixed solution is 1000 μM. Put the glass bottle into a shaking box at 20°C, stir and shake at a slow speed for 1 minute to form phosphorescent copper nanoclusters. The prepared copper nanoclusters were measured by a fluorescence spectrophotometer to draw a fluorescence curve, such as figure 2 .
Embodiment 3
[0059] Embodiment 3: get DMF and deionized water configuration 5mL mixed solution in 20mL glass bottle, DMF / H 2 O volume ratio is 2:1. Prepare 0.1M glutathione solution, add 200 μL of glutathione solution to the above mixed solution, shake gently until uniform, and the concentration of GSH in the mixed solution is 4 mM. Then configure 1M copper sulfate solution, take 5 μL of copper sulfate solution into the glass bottle, so that the concentration of copper ions in the mixed solution is 1000 μM. Put the glass bottle into a shaking box at 20°C, stir and shake at a slow speed for 1 minute to form phosphorescent copper nanoclusters. The prepared copper nanoclusters were measured by a fluorescence spectrophotometer to draw a fluorescence curve, such as image 3 .
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