Method for detecting Nosema bombycis spores in lepidopteran insects, staining solution and preparation method thereof

Abstract

The invention discloses a staining solution for Nosema bombycis spores in lepidopteran insects, which is a phosphate buffer containing isothiocyanate. Accordingly, the inventor also establishes a method for preparing the staining solution, and a detection method using the staining solution. In the detection method, a sample is fluorescently stained by the above staining solution, and then the spores are observed under a fluorescence microscope. The staining solution is used to fluorescently stain shells of the Nosema bombycis spores in the lepidopteran insects, and slides are easy to manufacture and easy to operate. The fluorescence microscope is used, and the staining solution used for staining the Nosema bombycis spores in the lepidopteran insects makes the Nosema bombycis spores in thelepidopteran insects have fluorescence specificity. The repeatability and accuracy of the detection are ensured by comparing with shape characteristics of a standard. In summary, the method for detecting the Nosema bombycis spores in the lepidopteran insects, the staining solution and the preparation method thereof have the characteristics of simple operation, strong specificity and easy identification, and lay a foundation for mechanical detection and fair detection, and have high practical value and broad market prospect.

Description

technical field [0001] The invention belongs to the field of lepidopteran insect detection and quarantine, and in particular relates to a detection method of lepidopteran insect microparticle protozoa spores, a staining solution and a preparation method thereof. Background technique [0002] Among the Lepidoptera insects, some are economic insects, such as silkworms and tussah silkworms. However, Lepidoptera insects can be infected with microparticle protozoon diseases, such as silkworm microparticle protozoon disease, tussah silkworm microparticle protozoon disease, castor silkworm microparticle protozoon disease, etc. Particulate protozoa are single-celled organisms that parasitize within cells. There are two main routes of infection: ingestion and germ infection. Particulate protozoa cause ingestion through host excrement and corpses; infection occurs through host germ embryos. The next generation is called germ-to-seed infection. [0003] Since the microparticle protoz...

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Problems solved by technology

Although the method has high accuracy, the operation is complicated and the cost is high, so it is difficult to be widely used in production.

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