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A kind of separation and purification of Rhododendron erinaceus mycorrhizal fungus strain dps-a and its inoculation application method

A technology of DPS-A and mycorrhizal fungi, applied in the direction of microorganism-based methods, biochemical equipment and methods, applications, etc., to achieve the effects of short strain isolation and purification cycle, simple technical operation, and improved seedling supply ability

Active Publication Date: 2022-04-29
浙江省大盘山国家级自然保护区管理局
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Problems solved by technology

[0006] In order to solve the deficiencies in the existing rhododendronaceous plant mycorrhizal fungus isolation and artificial cultivation technology, the purpose of the invention is to provide a kind of bacterial strain isolation and purification cycle is shorter, the technical operation is simple and convenient, realized the separation of rhododendron erinaceus mycorrhizal fungi and Isolation and purification of Rhododendron erinaceus mycorrhizal fungus strain DPS-A with high purification efficiency and large-scale operation and its inoculation application method

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  • A kind of separation and purification of Rhododendron erinaceus mycorrhizal fungus strain dps-a and its inoculation application method
  • A kind of separation and purification of Rhododendron erinaceus mycorrhizal fungus strain dps-a and its inoculation application method
  • A kind of separation and purification of Rhododendron erinaceus mycorrhizal fungus strain dps-a and its inoculation application method

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[0042] In order to make the technical means, creative features, goals and effects achieved by the present invention easy to understand, the present invention will be further described below in conjunction with specific examples. However, those skilled in the art will understand that the following examples are only used to illustrate the present invention and should not be considered as limiting the scope of the present invention. Those who do not indicate the specific conditions in the examples are carried out according to the conventional conditions or the conditions suggested by the manufacturer. The drugs and reagents used are not indicated by the manufacturer, and they are all conventional products that can be purchased through market sales.

[0043] A method for the isolation and purification of Rhododendron erinaceus mycorrhizal fungus strain DPS-A and its inoculation application method, comprising the following steps:

[0044] (1) Mycorrhizal selection and weighing: wa...

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Abstract

The invention discloses the separation and purification of Rhododendron erinaceus mycorrhizal fungus strain DPS‑A and its inoculation application method, comprising the following steps: (1) selecting mycorrhiza and weighing; (2) sterilizing the surface of the root system; (3) (4) root tissue homogenate; (5) strain purification; (6) preliminary screening of strains; (7) preparation of bacterial agents; (8) strain inoculation; (9) detection of inoculation effects. The advantages are: the strain isolation and purification period is short, the technical operation is simple, and the high efficiency and large-scale operation of the isolation and purification of Rhododendron erinaceus mycorrhizal fungi are realized.

Description

technical field [0001] The invention relates to the separation and purification of Rhododendron erinaceus mycorrhizal fungus strain DPS-A and its application method for inoculation. Background technique [0002] Mycorrhiza is a symbiosis formed by the vegetative roots of higher plants and mycorrhizal fungi, and is a very common natural phenomenon. Mycorrhizae can expand the nutrient absorption surface area of ​​the host plant root system, promote the production of various growth stimulating substances such as auxin, enhance the resistance of plant roots to diseases and insect pests, and improve the stress resistance of plants to harmful substances such as heavy metals, etc., thereby promoting plant growth. Individual growth and increase its resilience. The root system of most Rhododendron (Ericaceae) plants does not have a root hair structure, but consists of a large number of slender fibrous hairy roots with a limited surface area for nutrient absorption, and thus has a hi...

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/14C12N1/02A01G7/06A01G17/00C12R1/645
Inventor 刘亚王盼陈子林倪喜中宗侃侃张岑容周钰鸿陈江芳李月灵包金亮
Owner 浙江省大盘山国家级自然保护区管理局
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