Albumin nanoprobe co-loaded with a plurality of Alzheimer-treating medicines and preparation method thereof

A technology of nano-albumin and therapeutic drugs, which can be used in drug combinations, pharmaceutical formulations, preparations for in vivo tests, etc., can solve the problems of poor efficacy and inability to trace Alzheimer's disease, and achieve low cytotoxicity Effect

Inactive Publication Date: 2019-05-03
TIANJIN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0005] In order to solve the problems existing in the prior art, the present invention provides a method for preparing albumin nanoprobes co-loaded with various drugs for the treatment of Alzheimer’s, which solves the poor effect of a single drug in the treatment of Alzheimer’s disease in the prior art , Problems that cannot be traced when conventional drugs are used for treatment

Method used

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  • Albumin nanoprobe co-loaded with a plurality of Alzheimer-treating medicines and preparation method thereof
  • Albumin nanoprobe co-loaded with a plurality of Alzheimer-treating medicines and preparation method thereof
  • Albumin nanoprobe co-loaded with a plurality of Alzheimer-treating medicines and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0035] 1) Preparation of drug-loaded bovine serum albumin nanoprobe: get 2ml of 10mg / ml bovine serum albumin aqueous solution, and make it uniform by ultrasonic. Slowly add 5 μl of 40 mg / ml donepezil solution in dichloromethane, 7 μl of 40 mg / ml clioquinol solution and 2 μl of 80 mg / ml cy5 solution with a pipette gun. After stirring evenly, quickly add 100 μl of glutaraldehyde crosslinking agent aqueous solution, and finally react at 700 rpm / min, 20°C under the same stirring conditions for 3 hours. After the reaction is finished, filter with a 220nm water filter. The filtrate was centrifuged at 8,000 rpm / min for 4 minutes, and washed 3 times with deionized water to obtain a drug-loaded bovine serum albumin nanoprobe loaded with donepezil, clioquinol and cy5.

[0036] 2) Prepare a sample of the prepared drug-loaded bovine serum albumin nanoprobe, and observe its morphology with a transmission electron microscope.

Embodiment 2

[0038] 1) Preparation of drug-loaded bovine serum albumin nanoprobe: get 2ml of 15mg / ml bovine serum albumin aqueous solution, and make it uniform by ultrasonic. Slowly add 5 μl of 50 mg / ml donepezil solution in dichloromethane, 10 μl of 50 mg / ml clioquinol solution and 1 μl of 80 mg / ml cy5 solution with a pipette gun. After stirring evenly, quickly add 70 μl of glutaraldehyde crosslinking agent aqueous solution, and finally react at 700 rpm / min, 20°C under the same stirring conditions for 3 hours. After the reaction is finished, filter with a 220nm water filter. The filtrate was centrifuged at 7,000 rpm / min for 4 minutes and washed once with deionized water to obtain a drug-loaded bovine serum albumin nanoprobe loaded with donepezil, clioquinol and cy5.

[0039] 2) Prepare a sample of the prepared drug-loaded bovine serum albumin nanoprobe, and observe its morphology with a transmission electron microscope.

Embodiment 3

[0041] 1) Preparation of drug-loaded bovine serum albumin nanoprobe: get 2ml of 10mg / ml bovine serum albumin aqueous solution, and make it uniform by ultrasonic. Slowly add 5 μl of 60 mg / ml donepezil solution in dichloromethane, 12 μl of 60 mg / ml clioquinol solution and 2 μl of 90 mg / ml cy5 solution with a pipette gun. After stirring evenly, quickly add 100 μl of glutaraldehyde crosslinking agent aqueous solution, and finally react at 700 rpm / min, 20°C under the same stirring conditions for 4 hours. After the reaction is finished, filter with a 220nm water filter. The filtrate was centrifuged at 6,000 rpm / min for 4 minutes and washed twice with deionized water to obtain a drug-loaded bovine serum albumin nanoprobe loaded with donepezil, clioquinol and cy5.

[0042] 2) Prepare a sample of the prepared drug-loaded bovine serum albumin nanoprobe, and observe its morphology with a transmission electron microscope.

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Abstract

An albumin nanoprobe co-loaded with a plurality of Alzheimer-treating medicines and a preparation method thereof are disclosed. The albumin nanoprobe that can be co-loaded with a plurality of medicines and that can be tracked can be prepared from albumin by regulating fluorochrome for entrapment and the ratio and a solvent of two treatment medicines. Prepared nanoparticles can achieve co-loading of a plurality of medicines, and have relatively low cytotoxicity. The position of the medicines can be tracked in real time by observing the distribution of cy5 dye in the nanoparticles.

Description

technical field [0001] The present invention relates to the technical field of nanomedicine, and more specifically relates to an albumin nanoprobe co-loaded with various Alzheimer's therapeutic drugs and a preparation method thereof. Background technique [0002] Alzheimer's disease is a degenerative disease of the central nervous system. As my country enters the stage of population aging, the number of patients with Alzheimer's disease has increased significantly, which has caused great harm to human health and social harmony. Due to the complex pathogenesis of Alzheimer's disease, the effect of single drug therapy is poor. Therefore, how to effectively realize the synergistic effect of multiple drugs (such as donepezil and clioquinol) is a key scientific issue to improve the therapeutic effect of Alzheimer's disease. [0003] The rapid development of nanotechnology has made it possible to design multiple drug co-loaded delivery systems to distribute drug molecules only t...

Claims

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Application Information

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IPC IPC(8): A61K49/00A61K31/47A61K31/445A61K9/51A61K47/42A61P25/28
Inventor 常津杨涵武晓丽陈星濛高俊潇段玥董国修
Owner TIANJIN UNIV
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