Targeted gold nano-cone composite material used for photothermal therapy, and preparation method and applications thereof

A composite material and photothermal therapy technology, applied in the fields of nanomaterials and biomedicine, can solve the problems of limiting the biological application of gold nanocones and increasing biological toxicity, and achieve simple preparation steps, good stability and high biocompatibility. Effect

Inactive Publication Date: 2019-05-03
HUBEI UNIV OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, surfactants (such as cetyltrimethylammonium bromide CTAB) usually introduced during the preparation of gold nanocones enhance their wate

Method used

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  • Targeted gold nano-cone composite material used for photothermal therapy, and preparation method and applications thereof
  • Targeted gold nano-cone composite material used for photothermal therapy, and preparation method and applications thereof
  • Targeted gold nano-cone composite material used for photothermal therapy, and preparation method and applications thereof

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0060] Embodiment 1: Preparation of targeting gold nanocone composite material

[0061] 1) Preparation of pretreated bovine serum albumin:

[0062] a. Take sodium borohydride, bovine serum albumin and ethanol solution by weight ratio 1:1:15; Wherein, the volume fraction of ethanol solution is 60%;

[0063] b. Sodium borohydride was dissolved in ethanol solution, then bovine serum albumin was added, and the temperature was heated in a water bath at 70°C for 1 hour to obtain a pretreated bovine serum albumin solution;

[0064] 2) Preparation of gold nanocones by seed-mediated method

[0065] a. Preparation of Au seeds: Add chloroauric acid to 10mL of sodium citrate solution, stir vigorously and add sodium borohydride, store at room temperature for 2h, and obtain seed solution; wherein, the moles of sodium citrate, sodium borohydride and chloroauric acid The ratio is 1:1:5;

[0066] b. Preparation of gold nanocone: mix chloroauric acid solution, silver nitrate solution and cet...

Embodiment 2

[0073] Example 2: Characterization of gold nanocone composites

[0074] The gold nanocone composite was analyzed by transmission electron microscopy (TEM), figure 1 (A) and (B) are the gold nanocones and the targeted gold nanocone composites, respectively. The edges of the targeted gold nanocones composites are clear under the transmission electron microscope, and the morphology of the gold nanocones is not affected after BSA wrapping and targeting modification; Gold nanocones, gold nanocones coated with bovine serum albumin and targeted gold nanocone composites were analyzed by ultraviolet spectroscopy (UV-vis), according to figure 2 It can be seen that the gold nanocone composite material has a red shift of about 15-25nm in the maximum absorption wavelength compared with the gold nanocone composite material; compared with the gold nanocone composite material coated with bovine serum albumin, the maximum absorption wavelength has a red shift of about 5-15nm redshift.

Embodiment 3

[0075] Example 3: In vitro photothermal performance evaluation of gold nanocone composites

[0076] The in vitro photothermal performance of gold nanocone composites was evaluated by MTT method, and the photothermal toxicity of gold nanocone composites to B16F10 cells under different powers and different irradiation times was investigated. The results are as follows: image 3 (A) and (B), the survival rate of cells decreases with the increase of light intensity, and also decreases with the increase of light time, which shows that the in vitro photothermal performance of the gold nanocone composite is good. Using a cell apoptosis kit to observe at a certain intensity infrared laser (3W / cm 2 ,808nm) at different times of 0, 1, 3, 5min irradiation, cell apoptosis, the results are as follows image 3 (C). It can be seen that the gold nanocone composite has a significant photothermal effect.

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Abstract

The invention discloses a targeted gold nano-cone composite material used for photothermal therapy, and a preparation method and applications thereof. The targeted gold nano-cone composite material comprises a gold nano-cone, the outer surface of the gold nano-cone is coated with pre-treated bovine serum albumin, and the surface of each gold nano-cone particle is modified by a targeting molecule.The targeted gold nano-cone composite material is used in the preparation of a product for photothermal therapy on tumors, wherein the tumor is skin cancer (including epidermis, skin appendages, melanocytes and hematopoietic tissues) and breast cancer. The gold nano-cone composite material prepared by the invention is protected by the pretreated bovine serum albumin (BSA), and has high biocompatibility and good stability. The gold nano-cone composite material prepared by the invention can modify a plurality of targeted biomolecules, and can realize precise photothermal therapy on tumors. The composite material is relatively simple in preparation steps and good in repeatability, can be used for early treatment of cancers, and is a new technical means for clinical cancer treatment.

Description

technical field [0001] The invention relates to nanomaterials and applications in the field of biomedicine, in particular to a targeted gold nanocone composite material for photothermal therapy and its preparation method and application. Background technique [0002] Malignant tumor is a disease with a high mortality rate, which is constantly endangering human health and even life, and has been widely concerned by people. The current clinical treatment of tumors includes surgical treatment, chemotherapy, radiotherapy and immunotherapy. Due to the limitations of these treatment methods, such as chemotherapy and radiotherapy, due to low treatment accuracy, the treatment efficiency is reduced, systemic adverse reactions are increased, and adjacent healthy tissues are damaged. And other issues. [0003] Photothermal therapy (PTT) is an emerging technology for minimally invasive treatment of tumors. It uses exogenous photothermal materials to target tumor tissue, and irradiates ...

Claims

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Application Information

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IPC IPC(8): A61K41/00A61K47/64A61P35/00
Inventor 祝红达徐肖迪梅菲肖湘衡叶天宇秦汤
Owner HUBEI UNIV OF TECH
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