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Nanoparticles targeting systemic multistage lymph nodes, preparation method and application thereof

A nanoparticle and lymph node technology, applied in the field of biological science and drug carrier, can solve the problem of indistinguishable dark part imaging, achieve deep-level targeted imaging capability, facilitate large-scale production, and uniform particle size.

Active Publication Date: 2019-04-30
HUAZHONG UNIV OF SCI & TECH +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] At the same time, MRI (Magnetic Resonance Imaging) has high imaging contrast and resolution, but the dark part contrast produced by single-mode MRI imaging sometimes cannot be distinguished from the normal dark part of the body

Method used

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  • Nanoparticles targeting systemic multistage lymph nodes, preparation method and application thereof
  • Nanoparticles targeting systemic multistage lymph nodes, preparation method and application thereof
  • Nanoparticles targeting systemic multistage lymph nodes, preparation method and application thereof

Examples

Experimental program
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Effect test

Embodiment 1

[0043] Example 1: Nanoparticles Targeting Systemic Multilevel Lymph Nodes

[0044] An embodiment of the present invention provides a nanoparticle targeting multilevel lymph nodes throughout the body, see Figure 5 As shown, nanoparticles include phospholipids, lipiodol, optical probes, and alpha-helical polypeptides. Phospholipid is one or more of DMPC, MHPC, DMPE, DSPE-PEG2000. The optical probe is a water-soluble optical probe or a fat-soluble optical probe. The water soluble optical probe is ICG. The α-helical polypeptide sequence is FAEKFKEAVKDYFAKFWD or a polypeptide containing the FAEKFKEAVKDYFAKFWD sequence. The amino acid sequence of the α-helical polypeptide is shown in SEQ ID NO.1 in the sequence listing. seefigure 1 As shown in the electron microscope schematic diagram of the nanoparticles, the size distribution of the nanoparticles is uniform. see figure 2 As shown in the particle size distribution diagram of the nanoparticles, the particle size of the nanop...

Embodiment 2

[0045] Example 2: Preparation of nanoparticles encapsulating water-soluble optical probe ICG and lipiodol

[0046] The embodiment of the present invention provides a preparation method of nanoparticles targeting multi-level lymph nodes in the whole body:

[0047] A1. Dissolve DMPC, MHPC, DMPE, DSPE-PEG2000 and lipiodol in a mixture of chloroform and methanol (volume ratio 2:1); in terms of parts by mass, DMPC is 6-8 parts, and MHPC is 1.5-2 parts , 2.5 to 3.5 parts of DMPE, 2.5 to 3.5 parts of DSPE-PEG2000, and 30 to 35 parts of lipiodol.

[0048] A2, vacuum drying

[0049] Place the mixed solution in step A1 in a round-bottomed flask, ultrasonicate in a water bath for 30s; then use a vacuum pump to extract the organic solvent, and stir with a magnetic stirrer while vacuuming; continue drying for 2 hours until the organic solvent is completely extracted;

[0050] A3, water bath

[0051] Place the round-bottomed flask extracted in step A2 in water at 50°C, and continue the w...

Embodiment 3

[0060] Example 3: Preparation of nanoparticles encapsulating fat-soluble optical probe DiR-BOA and lipiodol

[0061] Steps refer to Example 2, the difference is that DiR-BOA is added to A1, wherein the α-helical polypeptide is 2 to 4 parts by mass, and the DiR-BOA is 0.24 to 0.3 parts, and the DiR -BOA was wrapped into nanoparticles, concentrated, purified in A7, and set aside.

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Abstract

The invention discloses nanoparticles targeting systemic multi-stage lymph nodes, which relates to the technical fields of biological science and drug carrier. The nanoparticles include phospholipids,iodized oil, optical probes and alpha-helical polypeptides, which have excellent physical and chemical properties and good biocompatibility. The invention also discloses a preparation method of nanoparticles for targeting systemic multistage lymph nodes, and the preparation process is simple. The invention also discloses an application of the nanoparticles targeting the systemic multi-stage lymphnode, the nanoparticles can be used for preparing a medicine, which carries two or more of a CT contrast agent, a fluorescent substance and a PET contrast agent to target the systemic multistage lymph nodes. The invention also discloses another nanoparticle targeting the systemic multi-stage lymph node, and the nanoparticle further comprises a DMPE-DTPA-Gd phospholipid derivative. The nanoparticle can be used to prepare the medicine that carries two or more of the CT contrast agent, the fluorescent substance, an MRI contrast agent, and the PET contrast agent to target the systemic multi-stagelymph node.

Description

technical field [0001] The invention relates to the technical fields of biological sciences and drug carriers, in particular to a nanoparticle targeting multilevel lymph nodes in the whole body, a preparation method and an application. Background technique [0002] The lymphatic vasculature, including the lymph nodes and the lymphatic vessels between them, plays an important role in the body. This system has strong permeability and transport capacity, and is mainly responsible for the collection of antigenic substances and the promotion of adaptive immune response under normal circumstances, including the promotion of antigen-specific T lymphocyte proliferation and antibody production. However, when tumor metastasis occurs, lymph nodes become one of the most likely tissues for metastatic tumor cells to gather due to the above characteristics. Visual analysis of lymph nodes with metastases in different periods is not only a tumor stage, but also an important indicator of pat...

Claims

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Application Information

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IPC IPC(8): A61K49/18A61K49/14A61K49/04A61K49/00
CPCA61K49/0002A61K49/0034A61K49/0093A61K49/0485A61K49/14A61K49/1869
Inventor 骆清铭张智红许国强钱源
Owner HUAZHONG UNIV OF SCI & TECH
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