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Proteolytic enzyme preparation applied for cancer treatment

A technology for proteolytic enzymes and cancer treatment, applied in the field of proteolytic enzyme preparations, can solve problems such as the lack of systematic development of cancer

Inactive Publication Date: 2019-04-30
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In China, the work of treating cancer with proteolytic enzymes has not been carried out systematically

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0011] Example 1: The killing effect of chymotrypsin preparation on cancer cells

[0012] The cancer cells were cultured together with normal tissues, the control group was treated with normal saline, and the experimental group was treated with chymotrypsin solution. In the control group, it can be seen that cancer cells have adverse effects on normal tissues. First, cancer cells penetrate into normal tissues during their own reproduction, and gradually crowd them out. After a few days, normal cells appear necrosis, cell lysis, and cancer cells multiply rapidly; In the group containing enzyme preparations, the situation was different. The cancer cells began to multiply in all directions, and the growth to the normal tissue was particularly fast, and then the growth stopped suddenly, some of them formed shafts or balls, and some curled up, the nucleus disappeared, and finally melted ; and the added enzyme preparations had no effect on normal tissues.

Embodiment 2

[0013] Embodiment 2: The curative effect of chymotrypsin preparation on mouse S180 sarcoma

[0014] (1) Animal selection: KM inbred mice, 22g±2g, single sex, 8 mice in each group.

[0015] (2) Grouping of animals: the experiment set up a blank control group, a high-dose chymotrypsin preparation group, a low-dose chymotrypsin preparation group, and a cyclophosphamide group

[0016] (3) Experimental method:

[0017] Mouse sarcoma (S180) in vitro cell line was resuscitated with liquid nitrogen, subcultured for several times, digested and centrifuged, and washed with 10 7 The concentration of cells per ml (0.2ml) was inoculated subcutaneously in healthy KM mice. After 15 days, a solid cancer with a size of one centimeter was formed. The nude mice were killed by neck dislocation, soaked in 70% alcohol for 1 minute, and the cancer was peeled off under sterile conditions and cut into 1mm 3 For tissue blocks of different sizes, select fresh cancer tissue without necrosis, wash it fi...

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PUM

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Abstract

The invention discloses a proteolytic enzyme preparation applied for cancer treatment. Healthy KM mice are inoculated subcutaneously with mice S180 sarcoma cells, and intramuscular administration is performed in a next day. The tumor inhibition rate of a high-dose group is 70% or more, and a new use of the proteolytic enzyme preparation in treatment of cancer is opened up.

Description

technical field [0001] The invention relates to a proteolytic enzyme preparation for cancer treatment, in particular to the application of chymotrypsin preparation in the treatment of cancer. Background technique [0002] Regarding the application of proteolytic enzymes in the treatment of cancer, in 1902 someone used pancreatic juice enzyme solution to treat various cancers and achieved certain results, which aroused the interest of many scholars and clinicians. It has been observed in many cases that the introduction of trypsin into the cancer quickly softens the tissue. [0003] In recent years, the data obtained from some foreign countries on the treatment of cancer with enzyme therapy shows that proteolytic enzymes have good clinical effects on various cancers. Local injection of enzyme preparations, and early use, with sufficient doses, and even continued use after physical symptoms have been significantly relieved, so that enzyme therapy will have a positive effect. ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K38/48A61P35/00
CPCA61K38/4826C12Y304/21001
Inventor 关茜茹
Owner 关茜茹
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