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Skin fibroblast precursor cell isolated culture and preparation preparing method

A precursor cell, separation and culture technology, applied in cell dissociation methods, biochemical equipment and methods, animal cells, etc., can solve the problem of the introduction of mycoplasma, virus and other pathogenic microorganisms from pigs, external pollution, and the introduction of mycoplasma from bovis , Viruses and other pathogenic microorganisms, etc., to achieve the effect of good ability, high safety, and ability to maintain

Pending Publication Date: 2019-04-23
王振坤
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  • Summary
  • Abstract
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AI Technical Summary

Problems solved by technology

[0004] There is a risk of exogenous contamination in the in vitro culture of skin fibroblast precursor cells. For example, the use of bovine serum in the culture system may introduce the risk of contamination by pathogenic microorganisms such as bovine mycoplasma and viruses. The use of porcine trypsin in the culture system may introduce porcine Risk of contamination by pathogenic microorganisms such as mycoplasma and virus

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0036] 1) Collect peripheral blood 60 in EDTA anticoagulation tube, transfer it to two 50ml centrifuge tubes in a biological safety cabinet. Centrifuge at 700g for 10 minutes, suck the upper yellow plasma into a clean 50ml centrifuge tube, and add normal saline to the remaining blood to the original volume.

[0037] 2) Add 15ml Percoll with a density of 1.057 to two 50ml centrifuge tubes, then slowly add 30ml of the peripheral blood diluted with the above physiological saline, and centrifuge at 500g for 20 minutes.

[0038] 3) Aspirate the middle and upper platelets into a clean 50ml centrifuge tube and centrifuge at 400g for 8 minutes.

[0039] 4) The supernatant was transferred to a clean 50ml centrifuge tube and centrifuged at 1600g for 5 minutes.

[0040] 5) Discard the supernatant and take the plasma from step 1 to resuspend the platelet pellet.

[0041] 6) Place the centrifuge tube containing platelet-rich plasma in liquid nitrogen for 30 seconds, and then thaw in a 37...

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PUM

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Abstract

The invention relates to the field of cell medical beauty, and discloses a skin fibroblast precursor cell isolated culture and preparation preparing method. Fibroblast precursor cells are obtained from the autologous skin tissue through a tissue block adherence method. Accordingly, autologous platelet-rich factor plasma is further utilized for expanding the autologous skin fibroblast precursor cells, and the sufficient amount of autologous skin fibroblast precursor cells are obtained. The skin fibroblast cells have the functions of secreting collagen and hyaluronic acid and other extracellularmatrices, and the good effect is achieved for wrinkle removing, soft tissue repairing and facial rejuvenation.

Description

technical field [0001] The invention relates to a method for separating and culturing skin fibroblast precursor cells and preparing a preparation. The invention belongs to the field of skin fibroblast precursor cell medical beauty treatment. Background technique [0002] There are a large number of fibroblasts in young skin, which can produce sufficient collagen, hyaluronic acid, elastic fibers and other substances, which well support the structure of the skin. However, with the increase of age, the various adult stem cells in the body gradually decrease, the fibroblasts in the skin begin to lose continuously, and the cell metabolism is weakened, and the corresponding substances such as collagen, elastic fibers, and hyaluronic acid will be Decreased, insufficient to support the structure of the skin, causing the dermis to thin, the skin begins to sag and wrinkles appear. Therefore, the decline in the vitality and number of fibroblasts is the root cause of skin aging. [0...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N5/077C12N5/078
CPCC12N5/0644C12N5/0656C12N2500/84C12N2509/00
Inventor 王振坤朱轶刘小龙
Owner 王振坤
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