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Preparation method of magnetic-metal organic framework MOF material

A metal-organic framework and magnetic technology, which is applied in the field of materials, can solve the problems affecting the enrichment and enrichment capacity and detection sensitivity of phosphopeptides, and achieve the ideal enrichment recovery rate, large pore volume, and high selectivity.

Pending Publication Date: 2019-04-23
ZHEJIANG FORESTRY UNIVERSITY
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Problems solved by technology

In addition, the various forms of affinity materials may affect the enrichment ability and detection sensitivity of phosphopeptide enrichment to a certain extent.

Method used

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  • Preparation method of magnetic-metal organic framework MOF material
  • Preparation method of magnetic-metal organic framework MOF material
  • Preparation method of magnetic-metal organic framework MOF material

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[0036]The embodiment of the present invention discloses a preparation method of a magnetic-metal organic framework MOF material, comprising the following steps: mixing oleic acid and ethanol under mechanical stirring, and then adding CaCl in sequence under vigorous stirring 2 Aqueous, aqueous NaOH and NaH 2 PO 4 ·2H 2 O aqueous solution, transferred to an autoclave, reacted at 160-200°C, washed to obtain hydroxyapatite nanowires; the hydroxyapatite nanowires and Fe(acac) were mixed under magnetic stirring 3 Disperse in triethylene glycol, then transfer to an autoclave, react at 200-240 ° C for 10-20 hours, wash, and obtain mag-HAP nanowires; alternately disperse the mag-HAP nanowires in FeCl 3 ·6H 2 In ethanol solution of O and 1,3,5-benzenetricarboxylic acid, incubate at 50-70°C for 10-20min respectively, in each cycle the intermediate is centrifuged at 3000-5000rpm, wash, repeat for 20-50 After several cycles of drying, a magnetic-metal organic framework MOF material was...

Embodiment 1

[0065] The mag-MOF nanofibers were prepared in three steps. First, inorganic hydroxyapatite nanowires were synthesized by solvothermal method as scaffolds. Specifically: 100 g of OA and 140 g of ethanol were mixed together under mechanical stirring. 200 mL of CaCl was added with vigorous stirring 2 (2.20 g) aqueous solution, 200 mL aqueous NaOH (10.0 g) and 100 mL NaH 2 PO 4 ·2H 2Aqueous O (2.80 g) was sequentially added to the mixture. The mixture was transferred to a 1 L Teflon-lined stainless steel autoclave and kept at 180 °C for 24 h. After naturally cooling to room temperature, the obtained HAP nanowire product was washed three times with ethanol and water, respectively.

[0066] Subsequently, the surface of the hydroxyapatite nanowires was functionalized with magnetic nanoparticles. Specifically: 1.0 g of hydroxyapatite nanowires and 0.5 g of Fe(acac) were mixed under magnetic stirring 3 Disperse in 180 mL TEG. The homogeneous mixture was transferred to six 100...

Embodiment 2

[0069] core-shell Fe 3 o 4 @MOF nanoparticles were prepared according to the prior art.

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Abstract

The invention provides a preparation method of a magnetic-metal organic framework MOF material. According to the preparation method of the magnetic-metal organic framework MOF material, an inorganic hydroxyapatite nanowire is used as a support; magnetic nano particles are functionalized on the surface of the hydroxyapatite nanowire; the hydroxyapatite nanowire is used as a template to perform theinduced assembly on the surface of the mag-HAP nanowire to form MOF nano crystals of Fe3O4. Firstly, the surface of the hydroxyapatite nanowire has rich active sites, and the magnetic nano particles directly grow on the surface, so that the agglomeration of the magnetic nano ions during the growth can be avoided. Secondly, the magnetic-metal organic framework MOF material has the characteristics of porous structure, large pore volume and good hydrophilcity, thereby improving the concentration efficiency and the concentration capability. Thirdly, the excellent super-paramagnetic magnetic response capability simplifies the separation process. The mag-MOF nano fibers prepared in the invention have high selectivity, low detection limit, ideal concentration recovery rate, excellent concentration capability and good reusability.

Description

technical field [0001] The invention relates to the field of material technology, in particular to a method for preparing a magnetic-metal organic framework MOF material. Background technique [0002] Selective capture and identification of targeted biomarkers from complex biological samples for early diagnosis of diseases is an attractive and important topic in biomedicine. As one of the most important post-translational modifiers, protein phosphorylation mediates a series of fundamental biological processes, such as protein folding, immune response, signal transduction and enzyme catalytic activity. The comprehensive analysis and identification of phosphorylated biomolecules can help to understand the biological response process and discover potential biomarkers of diseases. However, direct characterization of phosphopeptides by mass spectrometry (MS) remains a great challenge due to the low content of phosphopeptides in the sample and the strong signal interference from ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): B01J20/22B01J20/30
CPCB01J20/226
Inventor 郇伟伟李洁
Owner ZHEJIANG FORESTRY UNIVERSITY
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