Intelligent pH-catalytic-response micro-nano robot and assembling method and application thereof
An assembly method and robot technology, which can be applied in drug combinations, pharmaceutical formulations, organic active ingredients, etc., can solve problems that have not been reported in research, and achieve the effects of good targeting, fast speed, and high drug load
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Embodiment 1
[0026] This embodiment provides a method for assembling an intelligent pH catalysis-responsive micro-nano robot, which includes the following steps:
[0027] Step 1. Succinylated β-lactoglobulin is modified to obtain succinylated β-lactoglobulin.
[0028] The specific process is as follows: first dissolve 100 mg of β-lactoglobulin in 10 mL of phosphate buffer (50 mM, pH=7.4). Then, with stirring at room temperature, 25 mg of succinic anhydride was slowly introduced. Sodium hydroxide solution (1M) was added to maintain the pH value at 7.5-8.5, and the solution was stirred for 1.5 h. Then the solution was transferred to a dialysis bag (5000-7500 NMWCO), and placed in a glass beaker filled with 1 L of ultrapure water (4°C), and dialyzed for 24 hours, during which the dialysate outside the bag was replaced every 3 hours. Finally, the resulting solution was transferred to a round-bottom flask to freeze-dry to obtain succinylated β-lactoglobulin, which was called succinylated β-la...
Embodiment 2
[0036] This example is an evaluation of the motion performance of the Cat-β@ZIF micro-nano robot prepared in Example 1.
[0037] In order to objectively evaluate the movement performance of the Cat-β@ZIF micro-nano robot prepared in Example 1, this example tested the autonomous movement of the Cat-β@ZIF micro-nano robot under different hydrogen peroxide concentrations and pH conditions. The real-time movement of individual microrobots in X and Y coordinates is recorded by utilizing Nanoparticle Tracking Analyzer (NTA) and provides individual particle movement analysis. And this data is further used to plot the mean square displacement (MSD) curve of the particle, and the diffusion coefficient of the robot particle is calculated with the obtained MSD value.
[0038] image 3 It is the motion performance analysis diagram of the Cat-β@ZIF micro-nano robot in the first embodiment of the present invention.
[0039] Such as image 3 as shown, image 3 (a) Diffusion coefficient m...
Embodiment 3
[0044] In this example, the Cat-β@ZIF micro-nano robot prepared in Example 1 is used as a carrier to load the main drug to prepare a tumor treatment drug. In this embodiment, the main drug is doxorubicin (DOX).
[0045] The preparation process of the tumor treatment drug is as follows:
[0046] Add 1mg DOX to 1mL Cat-β@ZIF micro-nano robot particle aqueous solution (1.724mM DOX), after stirring slowly for 1h, centrifuge to remove the supernatant, and then wash to obtain the tumor treatment drug, which is loaded with DOX's Cat-β@ZIF micro-nano robot.
[0047] The DOX concentration in the supernatant was calculated from the concentration-absorbance standard curve, and the DOX loading rate of the Cat-β@ZIF micro-nano robot was as high as 83%.
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