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Kit for identifying benign and malignant human thyroid nodules and use method and application thereof

A technology for thyroid nodules and kits, which is applied in biochemical equipment and methods, and the determination/examination of microorganisms, can solve the problems of high positive rate, low probability of negative missed diagnosis, unclear diagnosis, etc., and achieves simple detection and easy operation. , reduce the effect of misjudgment

Pending Publication Date: 2019-04-19
上海安甲生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] However, in order to simultaneously detect somatic mutations of multiple thyroid cancer-specific pathogenic genes in thyroid nodule samples, it is necessary to break through the following scientific and technical difficulties: First, although a large number of thyroid Cancer-causing gene mutations, but among these reported mutated genes, it is unclear which are the real causative genes
Secondly, among the currently reported thyroid cancer pathogenic genes, it is not known which gene combinations have a high positive rate in the differential diagnosis of thyroid nodules and a low possibility of negative missed diagnosis

Method used

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  • Kit for identifying benign and malignant human thyroid nodules and use method and application thereof
  • Kit for identifying benign and malignant human thyroid nodules and use method and application thereof
  • Kit for identifying benign and malignant human thyroid nodules and use method and application thereof

Examples

Experimental program
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Effect test

Embodiment 2

[0097] The difference from Example 1 is that the PCR reaction system is shown in Table 7 below, the components and contents of PCR buffer 1-PCR buffer 7 are shown in Table 8 below, and the sample DNA of thyroid nodules is specifically analyzed by pathology The DNA content of the fresh pathological tissue determined to be benign thyroid nodules was 40ng, and the tested object was the same patient. image 3 As shown, the running tape of each gene segment in the electrophoresis graph is bright and clear, but a little blurred than that of Example 1. The electropherogram is basically consistent with that of Example 1, and the measured gene sequence is also the same as that of Example 1.

[0098] Table 7 PCR reaction system

[0099]

[0100] The composition and content of table eight PCR buffer 1-PCR buffer 7

[0101]

Embodiment 3

[0103] The difference from Example 1 is that the PCR reaction system is shown in Table 9 below, the components and contents of PCR buffer 1-PCR buffer 7 are shown in Table 10 below, and the sample DNA of thyroid nodules is specifically analyzed by pathology The DNA content of the postoperative frozen tissue determined to be benign thyroid nodules was 100ng, and the tested object was the same patient. Figure 4 As shown, the running tape of each gene segment in the electrophoresis graph is bright and clear, and the electrophoresis graph is basically consistent with that of Example 1, and the measured gene sequence is also the same as that of Example 1.

[0104] Table 9 PCR reaction system

[0105]

[0106] Table ten PCR buffer 1-PCR buffer 7 components and content

[0107]

[0108]

[0109] 1.4, Embodiment 4

[0110] The difference from Example 1 is that the PCR reaction system is shown in Table 11 below, the components and contents of PCR buffer 1-PCR buffer 7 are s...

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Abstract

The invention belongs to the technical field of kits, and in particular relates to a kit for identifying benign and malignant human thyroid nodules and a use method and application thereof. The kit comprises a sequencing primer system, a PCR buffer system, Taq enzyme, UNG enzyme and a negative quality control substance. The sequencing primer system comprises a primer pair BRAF-2, a primer pair KRAS-2, a primer pair NRAS-2, a primer pair HRAS-3, a primer pair HRAS-4 and a primer pair TERT-2, which are used for measuring 19 mutation sites on a BRAF gene, a KRAS gene, a NRAS gene, a HRAS gene anda TERT gene. The kit can be used for simultaneously detecting a plurality of gene variations, the detection result can be taken as an intermediate result to provide assistance for clinically diagnosing benign and malignant thyroid nodules and judging prognosis, and the kit can be further combined with other results to verify whether the thyroid cancer is suffered or not, so that probability of misjudgment is effectively reduced.

Description

technical field [0001] The invention belongs to the technical field of kits, and in particular relates to a kit for distinguishing benign from malignant human thyroid nodules and its use and application. Background technique [0002] Thyroid nodules are a common thyroid disease in the population. Most nodules are benign and can be treated conservatively, but 5%-10% of thyroid nodules are malignant and require early surgical treatment. to obtain a good prognosis. If a large number of patients with benign thyroid nodules who do not need surgery are given surgical treatment, a huge medical insurance burden will be generated; on the contrary, if the real malignant tumor cannot be identified from a large number of benign thyroid nodules, the treatment of these patients will be delayed, endangering patient's life and health. Therefore, timely and accurate detection of benign and malignant thyroid nodules is very important. [0003] With the development of molecular biology tech...

Claims

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Application Information

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IPC IPC(8): C12Q1/6883C12Q1/686
CPCC12Q1/686C12Q1/6883C12Q2600/118C12Q2600/16C12Q2600/156C12Q2565/125C12Q2535/122C12Q2537/143
Inventor 刘磊
Owner 上海安甲生物科技有限公司
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