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Method of preparing nano selenium from camellia plant polysaccharides and nano selenium prepared thereby

A plant polysaccharide and nano-selenium technology, which is applied in the field of nano-selenium, can solve the problems that the stability effect is not as good as that of nano-aggregates, and the stability is not ideal.

Active Publication Date: 2019-04-19
SOUTH CHINA AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

But, there are following problems and shortcomings in this invention: the water extract of camellia plant has the problem of combining alkaloids and polyphenols, and the stabilizing effect is not as good as that of nano-aggregates, but there is a problem of combining polyphenols and proteins in nano-aggregates, so the stability It is not ideal either; the substance that stabilizes nano-selenium at the core of camellia plant nano-aggregates is its polysaccharide-protein complex, so if it can be separated and extracted to stabilize nano-selenium, the effect may be better
[0008] In the prior art, there is no report on the method of preparing nano-selenium using camellia plant polysaccharides

Method used

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  • Method of preparing nano selenium from camellia plant polysaccharides and nano selenium prepared thereby
  • Method of preparing nano selenium from camellia plant polysaccharides and nano selenium prepared thereby
  • Method of preparing nano selenium from camellia plant polysaccharides and nano selenium prepared thereby

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Embodiment 1

[0036] Embodiment 1: A kind of method that utilizes camellia plant polysaccharide to prepare nano selenium.

[0037] (1) Accurately weigh 3.0g of Pu’er tea into a 500mL Erlenmeyer flask, add 240mL of ethanol with a volume concentration of 80%, shake in a shaker at 30°C and 200r / min for 24h, and after suction filtration, dry the tea leaves in an oven at 60°C .

[0038] (2) Pour the tea leaves into a beaker with a magnetic stirrer, add 60mL of water, put it in a magnetic stirring water bath at 80°C, stir in the water bath for 1.5h, extract twice, combine the filtrates, and depressurize at 60°C Concentrate to 1 / 10 of the original volume, then add 80% ethanol with a volume concentration three times the volume of the concentrated solution, and stand at 4°C for 24 hours.

[0039] (3) Pipette 30 mL of the liquid into a 50 mL centrifuge tube, centrifuge at 4°C, 4400 g for 10 min; discard the supernatant, dissolve the precipitate in water, freeze-dry to obtain a lyophilized powder, an...

Embodiment 2

[0043] Embodiment 2: A kind of method that utilizes camellia plant polysaccharide to prepare nano-selenium.

[0044] Compared with embodiment 1, the difference only lies in:

[0045] Weigh 25 mg (500 mg / L) of Puer tea polysaccharide freeze-dried powder into 50 mL centrifuge tubes, transfer to a beaker with 15 mL ultrapure water, add 9 mL ultrapure water, and stir evenly. The concentration of sodium selenite in the fixed system is 1mM. According to the ratio of reactants, Vc:sodium selenite 8:1 (mmol:mmol) is added successively with a certain amount of 100mM sodium selenite solution and a certain amount of 500mM vitamin C solution , adjust the order of adding, after the first reactant is added, mix well and let stand for 15min, then, mix well, react and let stand for 1h, to obtain nano-selenium colloidal solution; measure its colloidal chemical properties, the result is as follows figure 2 As shown, comprehensive consideration, first add a certain amount of 100mM sodium selen...

Embodiment 3

[0046] Embodiment 3: A kind of method that utilizes camellia plant polysaccharide to prepare nano selenium.

[0047] Compared with embodiment 1, the difference only lies in:

[0048] Weigh 12.5, 25, 37.5, 50 mg (250, 500, 750, 1000 mg / L) of Puer tea polysaccharide freeze-dried powder into 50 mL centrifuge tubes, transfer to a beaker with 15 mL ultrapure water, add 9 mL ultrapure water, and stir uniform. The concentration of sodium selenite in the fixed system is 1mM. According to the ratio of reactants, add a certain amount of 100mM sodium selenite solution to Vc:sodium selenite 8:1 (mmol:mmol), mix well and let stand for 15min, then add A certain amount of 500mM vitamin C solution was mixed evenly, and the reaction was allowed to stand for 1h to obtain a nano-selenium colloidal solution; the chemical properties of the colloid were measured, and the results were as follows: image 3 As shown, comprehensive consideration, when the template concentration is 500mg / L, the averag...

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Abstract

The invention belongs to the technical field of nano materials, and discloses a method of preparing nano selenium from camellia plant polysaccharides and a nano selenium prepared thereby. The method comprises following steps: (1) camellia plant polysaccharide separation: preparing a camellia plant water extract, and carrying out alcohol precipitation to obtain polysaccharide precipitates; and (2)preparing nano selenium from the camellia plant polysaccharides: carrying out reduction reactions between sodium selenite and vitamin C in the presence of camellia plant polysaccharides to obtain nanoselenium sol, and removing vitamin C to obtain a finished product of nano selenium. Camellia plant polysaccharides are taken as the template, the difference between nano selenium of different camellia plant polysaccharides is disclosed, the functions of nano selenium are enhanced, the application range is enlarged, and the application value is increased.

Description

technical field [0001] The invention belongs to the technical field of nanomaterials, and in particular relates to a method for preparing nano-selenium from camellia plant polysaccharides and the prepared nano-selenium. Background technique [0002] Selenium (Se) is one of the essential trace elements for the human body and is closely related to human health. Anti-oxidation and anti-cancer are the main biological activities of selenium. Selenium also has the functions of improving immunity, antagonizing heavy metals, participating in iodine metabolism in the body, regulating the level of iodine and free radicals in the body, etc. Selenium is a double-edged sword. It exerts anti-oxidant and anti-cancer activities but also produces toxicity. Biological effects of selenium and toxicity of selenium The concentration range of selenium is extremely narrow, and the toxicity is shown in a little more than the nutritional dose. The study found that the toxicity of selenium and sel...

Claims

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Application Information

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IPC IPC(8): C01B19/02B82Y30/00A61K33/04A61P35/00A61P39/06A61P37/04
CPCA61P35/00A61P37/04A61P39/06A61K33/04B82Y30/00C01B19/02
Inventor 林晓蓉穆静静李斌陈忠正张媛媛叶锡光高雄
Owner SOUTH CHINA AGRI UNIV
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