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Culture liquid and culture method for improving development quality of bovine fertilized embryo in vitro

A culture method and technology for fertilized embryos, which are applied in biochemical equipment and methods, culture process, tissue culture, etc., can solve the problems of insufficient quality and quantity, restrict wide application, and low number of blastocyst cells, and achieve lower production costs, The effect of improving growth rate, improving growth rate and development quality

Active Publication Date: 2019-04-16
NORTHWEST A & F UNIV
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Problems solved by technology

However, the quality and quantity of embryos cultured in vitro so far are often inferior to embryos that develop normally in vivo. In vitro fertilized embryos develop slowly in the stage of embryo compaction, resulting in a gradual decrease in the cleavage rate of embryos and a small number of cells forming blastocysts, which in turn damages Embryo quality affects the in vitro development of early embryos, which significantly restricts the wide application of this technology

Method used

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  • Culture liquid and culture method for improving development quality of bovine fertilized embryo in vitro

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Embodiment 1

[0023] (1) Source and preparation of reagents

[0024] Trypsin, EDTA, gentamicin, cephalosporin, inorganic salts, and paraffin oil (M8410) are products of sigma;

[0025] The M199 basal culture medium without Hepes is a product of Thermo Company;

[0026] Extra-grade fetal bovine serum (FBS) is a product of Gibco;

[0027] Essential amino acids are products of Thermo Fisher (product number 1831512);

[0028] Non-essential amino acids are products of Thermo Fisher (product number 1958909);

[0029] Fatty acid-free bovine serum albumin EFAF-BSA is a product of sigma (product number A6003-5G);

[0030] Insulin-transferrin-selenium additive ITS-G is a product of Thermo Fisher (product number 1865342, insulin 1g / L, transferrin 0.55g / L, selenium 0.00067g / L);

[0031] β-mercaptoethanol is a product of Thermo Fisher (Product No. 1922445);

[0032] Hypotaurine is a product of sigma company (article number H1384);

[0033] 1-oleoyl-sn-glycerol-3-sodium phosphate is a product of En...

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Abstract

The invention disclosesculture liquid for improving the development quality of a bovine fertilized embryoin vitro. The culture liquid comprises the following additives of an insulin-transferrin-selenium additive, beta-mercaptoethanol, hypotaurine, 1-oleoyl-sn-glycerol-3-sodium phosphate and salidroside. A culture method for improving the development quality of the bovine fertilized embryoin vitroby using the culture liquid is disclosed. The culture liquid and the culture methodcan effectively improve the development rate and development quality of the bovine fertilized embryoin vitro.

Description

technical field [0001] The invention relates to the field of livestock embryo engineering, in particular to a culture solution and a culture method for improving the development quality of bovine in vitro fertilized embryos. Background technique [0002] The in vitro culture of livestock embryos is a technology with potential research value and commercial value, which can be widely used in in vitro transplantation of embryos, animal gene editing and breeding, and the expansion of superior livestock breeds. However, the quality and quantity of embryos cultured in vitro so far are often inferior to embryos that develop normally in vivo. In vitro fertilized embryos develop slowly in the stage of embryo compaction, resulting in a gradual decrease in the cleavage rate of embryos and a small number of cells forming blastocysts, which in turn damages Embryo quality affects the in vitro development of early embryos, which significantly restricts the wide application of this technolo...

Claims

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Application Information

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IPC IPC(8): C12N5/073
CPCC12N5/0604C12N2500/25C12N2500/32C12N2500/33C12N2500/36C12N2500/44C12N2501/998C12N2501/999
Inventor 张涌王永张敏张景程王勇胜
Owner NORTHWEST A & F UNIV
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