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Antibacterial property test method of wet wipe products

A test method, technology of wet wipes, applied in the field of health care, can solve problems such as the inability to investigate antibacterial effects

Pending Publication Date: 2019-04-12
YANGZHOU PERFECT DAILY CHEM
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Therefore, it is impossible to investigate the antibacterial effect of wet wipes with non-woven fabrics

Method used

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  • Antibacterial property test method of wet wipe products
  • Antibacterial property test method of wet wipe products
  • Antibacterial property test method of wet wipe products

Examples

Experimental program
Comparison scheme
Effect test

preparation example Construction

[0027] 1. Preparation of bacterial suspension

[0028] Activate Staphylococcus aureus, Escherichia coli and Pseudomonas aeruginosa on the TSA plate for 18-24 hours respectively, at a culture temperature of 30-35°C;

[0029] Then use 0.85% sterile normal saline to elute the above three strains respectively, and prepare 5.0×10 6 cfu / ml bacterial suspension;

[0030] Finally, all bacterial suspensions were mixed to prepare 5.0×10 6 cfu / ml mixed bacterial suspension.

[0031] 2. Preparation of yeast suspension

[0032] Cultivate Candida albicans on SDA medium for 48 hours at a temperature of 20-25°C;

[0033] Then the Candida albicans strains were eluted with 0.85% sterile saline, and prepared into 5.0×10 5 cfu / ml bacterial suspension.

[0034] 3. Preparation of mold suspension

[0035] Cultivate Aspergillus niger species on SDA medium for 7 days at a culture temperature of 20-25°C;

[0036] Then with 0.85% sterile normal saline plus Tween 80 with a volume concentration of...

Embodiment 1

[0072] In Example 1, the wet tissue sample used was 5g / piece*3 pieces / pack, and the volume Vi of the inoculated bacterial suspension on each wet tissue sample=3×5g×0.3ml / g=4.5ml;

[0073] The volume of diluent added E = 5g x 3 x 10ml / g - 5g x 3 x 1ml / g - 4.5ml = 130.5ml.

[0074] Table 1 is the parameter of three test bacterial suspensions of embodiment 1.

[0075] Table 1

[0076]

[0077] Table 2 shows the number of colonies of the wet tissue samples of Example 1 at various time points during the test.

[0078] Table 2

[0079]

[0080] It can be seen from Table 2 that the number of colonies of bacteria on and after the 7th day was 0, reducing by 5 log levels, meeting the antibacterial performance requirements; the number of colonies of yeast on and after the 14th day was 0, reducing by 4 log levels, It meets the antibacterial performance requirements; after the 14th day, the number of colonies of mold is 10, a reduction of 2 log levels, which meets the antibacteria...

Embodiment 2

[0082] In Example 2, the wet tissue sample used is 16.5g / piece*1 piece / pack, and the volume Vi of the inoculated bacterial suspension on each wet tissue sample=16.5g×0.3ml / g=4.95ml;

[0083] The volume of diluent added E = 16.5g x 10ml / g - 16.5g x 1ml / g - 4.95ml = 143.55ml.

[0084] Table 3 is the parameter of three test bacterial suspensions of embodiment 2.

[0085] table 3

[0086]

[0087]

[0088] Table 4 shows the number of colonies of the wet tissue samples of Example 2 at various time points during the test.

[0089] Table 4

[0090]

[0091] It can be seen from Table 4 that the number of colonies of bacteria on and after the 7th day was 0, reducing by 5 log levels, meeting the antibacterial performance requirements; the number of colonies of yeast on and after the 14th day was 0, reducing by 4 log levels, It meets the antibacterial performance requirements; the number of mold colonies after the 14th day is 8, a reduction of 2 log levels, which meets the an...

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Abstract

The invention belongs to the technical field of hygiene care, and provides an antibacterial property test method of wet wipe products. The antibacterial property test method comprises the following steps of selecting wet wipe samples; inoculating the wet wipe samples with a bacterium suspension containing pathogenic bacteria, and performing primary culturing; regularly extracting the bacterium suspension in the wet wipe samples, performing secondary culture, and calculating the clump count of the pathogenic bacteria in the bacterium suspension after secondary culture; and judging whether reduction of the clump count of the pathogenic bacteria conforms to antibacterial property requirements or not. The antibacterial property test method is designed in accordance with preparation formulationof wet wipe products, a form that microorganisms can be carried in the actual using process is simulated, and representative pathogenic bacteria are used for inoculating. Test results are subjected to trend analysis, and whether the bacterial inhibition properties of the wet wipe products conform to the requirement is judged.

Description

technical field [0001] The invention relates to the technical field of health care, in particular to a method for testing the antibacterial performance of a wet tissue product. Background technique [0002] In recent years, because wet wipes are more convenient to carry and use, they have become an essential daily necessities for most people in their homes, trips, and offices. Especially for families with children, convenient and easy-to-use wet wipes, together with milk powder and diapers, have become the necessities of raising children in modern families. [0003] The use characteristics of wet wipes determine that conventional wet wipes must not only inhibit the reproduction of their own microorganisms, but also inhibit the reproduction of microorganisms brought in from the outside during use. [0004] The test samples for the antibacterial efficacy test in the current domestic and international regulations are all for liquid or paste formulations, not for wet wipes (non...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/18C12Q1/14C12Q1/10C12Q1/04C12R1/445C12R1/19C12R1/385C12R1/685C12R1/725
CPCC12Q1/04C12Q1/10C12Q1/14C12Q1/18G01N2333/21G01N2333/245G01N2333/31G01N2333/38G01N2333/40
Inventor 俞晨波
Owner YANGZHOU PERFECT DAILY CHEM
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