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Method for counting bacterial colonies in fermented feed

A technology for fermenting feed and feed, which is applied to biochemical equipment and methods, and the determination/inspection of microorganisms, can solve the problems of inaccurate counting, affecting the counting of lactic acid bacteria and yeast, and achieve the effect of improving counting accuracy and easy operation.

Inactive Publication Date: 2019-04-12
JILIN COFCO BIOCHEM +2
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Due to the strong stress resistance of spores, and the colony is easy to grow in other media, which affects the count of lactic acid bacteria and yeast, the current detection method (such as the national standard method [1] GBT13093-2006) cannot accurately count, and this value The accuracy is directly related to the quality of fermented feed

Method used

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  • Method for counting bacterial colonies in fermented feed
  • Method for counting bacterial colonies in fermented feed
  • Method for counting bacterial colonies in fermented feed

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0043] Embodiment 1: the enumeration of three kinds of strains in fermented feed 1

[0044] Weigh 10g of fermented feed 1 and add it to 90mL sterile saline and mix well to obtain 10 -1 The sample suspension, draw 1mL of the sample suspension and dilute it 10 times with sterile normal saline, and obtain dilutions of 10 times in turn. 2 、10 3 、10 4 、10 5 、10 6 times the sample dilution.

[0045] Draw 1mL respectively and the dilution factor is 10 4 、10 5 、10 6 and added to the center of 3 sterile plates with a diameter of 90mm (three repetitions were set up for each concentration of sample dilution, a total of 9 plates), and 20mL of 50°C containing 500ppm Natal was added to each plate. MRS medium of mycin, mix well, cool until it solidifies completely, then place it upside down in a constant temperature incubator at 37°C for about 48 hours, and take out the plate when clearly separated colonies can be seen with the naked eye.

[0046] Draw 1mL respectively and the dil...

Embodiment 2

[0053] Example 2: Enumeration of three bacterial strains in fermented feed 2

[0054] Except that fermented feed 1 in embodiment 1 was replaced by fermented feed 2, the same method as in embodiment 1 was used to count lactic acid bacteria, bacillus and yeast.

[0055] The result is as Figures 5A-5C As shown, most of the colonies on the MRS medium plate are lactic acid bacteria colonies, and the number of viable bacteria is 1.0×10 8 cfu / mL( Figure 5A left). Most of the colonies on the LB medium plate are Bacillus colonies, and the number of viable bacteria is 3.0×10 8 cfu / mL( Figure 5B left). The colonies on the YPD solid medium plate are all yeast colonies, and the number of viable bacteria is 1.6×10 8 cfu / mL( Figure 5C left).

[0056] The national standard method [1] GBT13093-2006 was used to count the lactic acid bacteria, bacillus and yeast in the same sample dilution. As a result, yeast and bacillus grew on the MRS medium plate and seriously affected the coun...

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Abstract

The present invention relates to a method for counting lactic acid bacteria, bacillus and yeast in a fermented feed. The method is as below: diluted fermented feed samples are separately counted in anMRS solid medium containing natamycin, a YPD medium containing streptomycin and penicillin, and an LB medium, so that the number of lactic acid bacteria, bacillus and yeast in the fermented feed canbe more accurately counted, and the counting accuracy of single microorganisms in the fermented feed is improved; and the method is easy to operate.

Description

technical field [0001] The invention relates to the field of microbial counting, in particular to a method for counting lactic acid bacteria, bacillus and yeast in fermented feed. Background technique [0002] Fermented feed is under artificial control conditions, through the metabolic activities of microorganisms themselves, the anti-nutritional factors in plant, animal and mineral substances are decomposed or transformed, and the resulting feed is more edible, digested and absorbed by livestock and poultry without toxic effects. feed ingredients. Studies have shown that fermented feed can improve its nutrient absorption level, degrade toxins that may exist in its raw materials, and promote growth, maintain micro-ecological balance in animals, enhance immunity, prevent and cure diseases, etc. reflect. [0003] Among them, microorganisms play a key role in this fermentation process. Therefore, one of the indicators for evaluating the quality of fermented feed is the numbe...

Claims

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Application Information

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IPC IPC(8): C12Q1/06
CPCC12Q1/06
Inventor 佟毅焦琳安泰郑晓卫陈博卢宗梅王博陈影金渭武
Owner JILIN COFCO BIOCHEM
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