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Wild chrysanthemum essential oil for controlling phytophthora and application thereof

A technology of wild chrysanthemum essence and Phytophthora, which is applied in the direction of application, chemicals for biological control, animal repellent, etc., can solve the problem of lack of plant prevention and control of Phytophthora pathogens, achieve good fumigation effect, inhibit spore germination, The effect of inhibiting mycelial growth

Active Publication Date: 2019-04-12
ZUNYI TOBACCO OF GUIZHOU TOBACCO CORP +1
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] In order to overcome the defect of the lack of vegetal prevention and control of Phytophthora pathogenic bacteria in the prior art, the present invention provides a kind of wild chrysanthemum essential oil for the prevention and treatment of Phytophthora, which has remarkable bacteriostasis effect on Phytophthora nicotiana and good antibacterial effect of fumigation

Method used

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  • Wild chrysanthemum essential oil for controlling phytophthora and application thereof
  • Wild chrysanthemum essential oil for controlling phytophthora and application thereof
  • Wild chrysanthemum essential oil for controlling phytophthora and application thereof

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Experimental program
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Effect test

Embodiment 1

[0046] 1. Plant material

[0047] Fresh wild chrysanthemums were collected from coastal beaches in Shandong in October 2017, brought back to the laboratory in nylon mesh bags, and dried in the shade for later use.

[0048] 2. Extraction

[0049] Grind the dried wild chrysanthemum flower buds into powder, sift through a 40-mesh sieve, weigh 100g, put it into a 2000mL beaker, add distilled water according to the ratio of material to liquid 1g:10mL, and place it in an ultrasonic container after standing at room temperature for 3 hours. 120W, ultrasonic time 30min, ultrasonic temperature 50°C to obtain the ultrasonic extraction mixture. The ultrasonic extraction mixture was subjected to steam distillation, the distillation time was 5 hours, the temperature of the oil-water separator was 20°C within half an hour of boiling, and then kept at 30°C, and the distillate was obtained after the distillation. The distillate and n-hexane were mixed and extracted at a volume ratio of 10:1,...

Embodiment 2

[0051] 1. Test process:

[0052] 1. Phytophthora nicotiana culture

[0053] For the strong pathogenicity of tobacco Phytophthora nicotianae JM01 strain culture and spore preparation methods refer to the literature method (Han, T.; You, C.; Zhang, L.; Feng, C.; Zhang, C.; Wang, J.; Kong, F. Biocontrol potential of antagonist Bacillus subtilis Tpb55 against tobacco black shank. BioControl 2016, 61, 195–205.).

[0054] 2. Determination of antibacterial activity

[0055] 2.1. Plate agar diffusion method

[0056] After Phytophthora nicotianae overgrows on the oat medium, pick the bacterium cake (5mm) on the edge of the colony and place it on one side of the plate, punch holes on the other side correspondingly, add 100 μL of the wild chrysanthemum essential oil prepared in Example 1 (the same below, No more details) (10 μl / mL), DMSO was used as the control, repeated three times, and then cultured at 28°C for 5 days, the diameter of the inhibition zone was measured, and the morpho...

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Abstract

The invention provides a wild chrysanthemum essential oil for controlling phytophthora and application thereof, and relates to the technical field of phytophthora pathogen control. The preparation method of the wild chrysanthemum essential oil for controlling phytophthora comprises the following steps that wild chrysanthemums and water are mixed and stood, the supersonic extraction is carried outto obtain an ultrasonic extraction mixture, stream distillation is performed on the ultrasonic extraction mixture, and distillate is collected; the distillate is extracted with normal hexane, organicphases are separated and dried to obtain wild chrysanthemum essential oil. The wild chrysanthemum essential oil has the advantages that the mycelial growth and spore germination of Phytophthora nicotianae are effectively inhibited, the minimum inhibition concentration can reach 200MuL / L, and the bacteriostasis rate under the concentration fumigation of 157.48MuL / L can reach 92.68%. The mechanism of bacteriostasis of the wild chrysanthemum essential oil may result from the incompleteness of cell walls and cell membranes. The wild chrysanthemum essential oil lays a foundation for the developmentof botanical pesticides for controlling phytophthora and even soil-borne diseases.

Description

technical field [0001] The invention relates to the technical field of Phytophthora prevention and control, in particular to a wild chrysanthemum essential oil for preventing Phytophthora and application thereof. Background technique [0002] Phytophthora nicotianae van Breda de Haan (syn.P.parasiticaDastur), a typical soil-borne pathogen, is now considered one of the most devastating oomycete diseases, capable of infecting more than 255 species of plants, including the severely damaged tobacco (Nicotiana spp.), tomato (Lycopersicum esculentum) and citrus (Citrus spp.), etc. Due to the wide distribution of host diversity and ecological regions, the huge loss caused by it is difficult to estimate, and it is a continuous challenge of plant disease control. The disease is currently mainly controlled by chemical pesticides such as metalaxyl, propamocarb, and mancozeb. The long service life of the pesticides is likely to lead to drug resistance of pathogenic bacteria, resulting ...

Claims

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Application Information

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IPC IPC(8): A01N65/12A01P3/00
CPCA01N65/00A01N65/12
Inventor 芶剑渝张成省刘明宏荆常亮韩小斌赵栋霖彭玉龙邹平刘京袁源温明霞王小彦张远淑祝乾湘罗玉英
Owner ZUNYI TOBACCO OF GUIZHOU TOBACCO CORP
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