Test strip for detecting influenza viruses and preparation method thereof
A type of influenza B virus and detection area technology, applied in the field of medical testing, can solve problems such as the inability to guarantee the quality of test units, poor sensitivity and repeatability, and affect test results, so as to facilitate clinical diagnosis, improve stability and sensitivity , the effect of easy operation
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[0044] A preparation method for preparing the time-resolved immunochromatographic test strip for detecting influenza A / B virus, comprising the following steps:
[0045] (1) On the reaction membrane 3, respectively immobilize the influenza A / B influenza monoclonal capture antibody and the goat anti-mouse IgG antibody recognizing a single epitope to form a detection area and a quality control area;
[0046] (2) Prepare time-resolved fluorescent microsphere-labeled influenza A / B influenza virus monoclonal detection antibody, and spray on the binding pad 2;
[0047] (3) Paste the sample pad 1, the binding pad 2, the reaction membrane 3 and the water-absorbing pad 4 on the bottom plate 5 in order to obtain the time-resolved fluorescent immunochromatographic test strip.
[0048] In a preferred embodiment, step (2) further includes the following steps:
[0049] (2.1) Use 0.01-0.05mol / L MES activation buffer of pH 5.5-6.0 to wash the microspheres, add carbodiimide (EDC) and N-hydroxy...
Embodiment 1
[0072] Example 1 Time-resolved immunochromatographic test strips for detecting influenza A / B viruses.
[0073] A time-resolved immunochromatographic test strip for detecting influenza A / B virus of the present embodiment, comprising a PVC bottom plate, and a sample pad, a binding pad, a reaction membrane and a water-absorbing pad sequentially arranged on the PVC bottom plate, The binding pad is coated with influenza A / B monoclonal detection antibody labeled with fluorescent microspheres, and the reaction membrane includes a detection area and a quality control area arranged in parallel and separated by 6 mm from each other, and the detection area is close to the The binding pad, the quality control area is close to the water-absorbing pad, the detection area is coated with a monoclonal capture antibody for influenza A / B that recognizes a single antigenic epitope, and the quality control area is coated with goat antibody Mouse IgG antibody.
[0074] In this embodiment, the reac...
Embodiment 2
[0083] Example 2 A time-resolved immunochromatographic kit for detecting influenza A / B viruses.
[0084] The time-resolved fluorescence immunochromatography kit for detecting influenza A / B virus of this embodiment, said kit includes: the test strip described in Embodiment 1, a plastic cartridge, and a sample buffer.
[0085] In this embodiment, the sample buffer contains 0.5% BSA, 0.05% Tween-20 in PBS pH7.4 buffer solution.
[0086] When the time-resolved fluorescent immunochromatography kit for detecting influenza A / B virus of the present invention is used, the throat swab sample to be tested is added to the sample buffer, and 50 μL of sample dilution is added dropwise on the sample pads of A and B respectively. liquid, which transports the sample to the conjugate pad by capillary action. When the throat swab sample contains influenza A / B virus, it forms an antigen-antibody complex with the monoclonal antibody on the fluorescent microspheres. With the action of chromatograp...
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