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A protein biomarker for the diagnosis of osteoporosis and its application

A biomarker and osteoporosis technology, applied in the field of protein biomarkers, can solve the problems of small OP heritability and unclear function of disease genes, and achieve excellent sensitivity and specificity

Active Publication Date: 2021-08-31
SUZHOU UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The combination of identified genetic susceptibility loci to explain OP heritability is small, a large number of genetic factors remain to be discovered, and the functions of known disease genes are mostly unclear

Method used

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  • A protein biomarker for the diagnosis of osteoporosis and its application
  • A protein biomarker for the diagnosis of osteoporosis and its application
  • A protein biomarker for the diagnosis of osteoporosis and its application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0029] Example 1: Screening of Dysregulated Proteins in Peripheral Blood Mononuclear Cells of Osteoporosis Patients (9:9)

[0030] 1. After obtaining informed consent, 9 patients with osteoporosis and 9 healthy individuals with bone were collected as normal controls. Fasting peripheral blood samples were collected and placed in sodium citrate anticoagulant tubes.

[0031] 2. Peripheral blood mononuclear cells were obtained by density gradient centrifugation and MACS positive sorting method, and the cell proteome was identified and quantified. Specific steps are as follows:

[0032] (1) Peripheral blood mononuclear cell isolation

[0033] 1. Add 15ml of density gradient separation solution to the leucosep tube, centrifuge at 100g for 1min.

[0034] 2. Pour the collected fresh whole blood into a 50ml centrifuge tube, add an equal amount of PBS and mix well, then transfer to a leucosep tube, and centrifuge at 310g for 20min.

[0035] 3. Aspirate and discard the remaining super...

Embodiment 2

[0080] Example 2: Screening of dysregulated proteins in peripheral blood mononuclear cells of patients with OF (18:18)

[0081] This example contains 18 OF patients and 18 controls with no history of fractures. Patients and controls were matched for age and sex. Peripheral blood was collected, blood mononuclear cells were isolated, total protein was extracted, enzyme digestion, identification and quantification, case-control differential analysis, identification of differentially expressed proteins.

[0082] The specific experiment and analysis method flow are the same as those in Example 1, and will not be repeated here. The analysis results showed that there was a significant difference in the expression level of ABI1 protein between the osteoporotic fracture group (OF) and the control group (NF) (P figure 2 (Right panel) shows dysregulated ABI1 protein expression in peripheral blood mononuclear cells of patients with osteoporotic fractures. As can be seen from the figure,...

Embodiment 3

[0083] Example 3: Validation of peripheral blood mononuclear cell protein markers in patients with osteoporosis

[0084] I. Identification of Osteoporosis Protein Markers

[0085] A total of 27 subjects were included in this example. Among them, 9 cases were individuals with normal bone density (CTRL), 9 cases were osteoporotic patients (OP), and 9 cases were osteoporotic fracture patients (OF). Peripheral blood was collected, blood mononuclear cells were separated, total cell protein was extracted, any three samples in the group were mixed as a small group, and the concentration was determined. The specific experiment and analysis method flow are the same as those in Example 1, and will not be repeated here.

[0086] image 3 The expression levels of ABI1 protein in peripheral blood mononuclear cells of individuals with normal bone density (CTRL), patients with osteoporosis (OP), and patients with osteoporotic fractures (OF) were significantly different (P=0.0053). It can...

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Abstract

The invention discloses a protein biological marker for diagnosing osteoporosis and its application, and belongs to the technical field of biological detection. The present invention uses Abl Interactor 1 protein as a biomarker for osteoporosis diagnosis. The protein biomarkers provided by the present invention can be used to prepare diagnostic kits, and have excellent diagnostic value when applied to blood samples to diagnose osteoporosis. Using the protein of the present invention alone, the diagnostic AUC can reach 0.76. Taking 0.376ng / ml as the cut-off point, the sensitivity and specificity were 73.3% and 64.3%, respectively. Combined with CTX and P1NP, the diagnostic AUC can reach 0.78. The protein can be used as a biomarker for the diagnosis of human osteoporosis blood samples, which is helpful for the diagnosis of osteoporosis, the identification of high-risk groups of osteoporotic fractures, and the prevention and treatment.

Description

technical field [0001] The invention relates to a protein biomarker for diagnosing osteoporosis and its application, belonging to the technical field of biological detection. Background technique [0002] Osteoporosis (Osteoporosis, OP) is a common chronic disease, and its clinical manifestations are short stature, low back pain, and low-trauma fractures (also known as osteoporotic fractures, OF). OF has a high morbidity rate, with a mortality rate as high as 31%. At present, the prevalence rate of osteoporosis among Chinese residents is as high as 8.8‰. Screening new biomarkers of OP, revealing its molecular pathogenic mechanism, establishing an early diagnosis and prediction model of OP, and developing targeted intervention measures are extremely important strategic measures to control OP. [0003] OP is a complex disease regulated by both environmental and genetic factors, among which genetic factors are an important factor affecting the pathogenesis of OP, accounting f...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N30/02G01N30/06
CPCG01N30/02G01N30/06
Inventor 邓飞艳雷署丰周旭
Owner SUZHOU UNIV
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