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Composite microbial remediation agent for black and odorous water body and application thereof

A technology for microbial remediation, black and odorous water bodies, applied in the directions of microorganisms, microorganism-based methods, biological water/sewage treatment, etc., it can solve the problems of limited degradation effect, limited rapid development of technical service market, and unclear ammonia nitrogen degradation indicators, etc. To achieve the effect of eliminating water odor

Inactive Publication Date: 2019-04-02
CHAMBROAD CHEM IND RES INST CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] Patents CN 107699513A and CN 107760625A respectively announced a special degrading bacterium for black and odorous water bodies and its application and a composite microbial bacterial agent for treating rivers with different pollution degrees, but the degradation effect was limited to the laboratory; patent CN 107267421 A announced a A compound bacterial agent suitable for black and odorous water treatment and its cultivation method, the ammonia nitrogen degradation index is not clear
The uneven effect of microbial remediation on black and odorous water restricts the rapid development of the technical service market, and it is urgent to develop efficient microbial agents in combination with the needs of water treatment in China

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0043] (1) Preparation of Bacillus subtilis bacterial agent

[0044] The composition of the liquid fermentation medium is by weight (w / w): glucose 1%, corn flour 1.0%, soybean meal 2%, urea 0.25%, manganese sulfate 0.01%, magnesium sulfate 0.04%, sodium chloride 0.04%, sulfuric acid Ferrous 0.004%, calcium chloride 0.02%, pH7.2;

[0045] Liquid fermentation conditions: the inoculum size is 5% (v / w), the culture temperature is 36°C, the shaker speed is 150r / min, and the culture time is 60h;

[0046] The content of Bacillus subtilis spores obtained by spray drying reaches 3×10 10 CFU / g of bacterial agent dry powder.

[0047] (2) Preparation of Pichia pastoris inoculant

[0048] The composition of the liquid fermentation medium is by weight (w / w): glucose 2.8%, soybean meal 2.4%, ammonium chloride 0.18%, magnesium sulfate 0.06%, potassium dihydrogen phosphate 0.11%, dipotassium hydrogen phosphate 0.06%, sulfuric acid Manganese 0.011%, pH6.0;

[0049] Liquid fermentation cond...

Embodiment 2

[0061] (1) Preparation of Bacillus subtilis bacterial agent

[0062] The composition of the liquid fermentation medium is by weight (w / w): glucose 1.4%, corn flour 0.8%, soybean meal 2.7%, urea 0.1%, manganese sulfate 0.021%, magnesium sulfate 0.08%, sodium chloride 0.025%, sulfuric acid Ferrous 0.003%, calcium chloride 0.028%, pH7.4;

[0063] Liquid fermentation conditions: the inoculum size is 9% (v / w), the culture temperature is 37°C, the shaker speed is 160r / min, and the culture time is 96h;

[0064] The content of Bacillus subtilis spores obtained by spray drying reached 7×10 10 CFU / g of bacterial agent dry powder.

[0065] (2) Preparation of Pichia pastoris inoculant

[0066] The composition of the liquid fermentation medium, by weight (w / w): glucose 2.5%, soybean meal 2.2%, ammonium chloride 0.3%, magnesium sulfate 0.04%, potassium dihydrogen phosphate 0.13%, dipotassium hydrogen phosphate 0.05%, sulfuric acid Manganese 0.012%, pH6.3;

[0067] Liquid fermentation c...

Embodiment 3

[0078] (1) Preparation of Bacillus subtilis bacterial agent

[0079] The composition of the liquid fermentation medium is by weight (w / w): glucose 2%, corn flour 1.1%, soybean meal 2.3%, urea 0.3%, manganese sulfate 0.03%, magnesium sulfate 0.06%, sodium chloride 0.03%, sulfuric acid Ferrous iron 0.006%, calcium chloride 0.03%, pH7.25;

[0080] Liquid fermentation conditions: the inoculum size is 7% (v / w), the culture temperature is 32°C, the shaker speed is 200r / min, and the culture time is 80h;

[0081] The content of Bacillus subtilis spores obtained by spray drying reaches 8×10 10 CFU / g of bacterial agent dry powder.

[0082] (2) Preparation of Pichia pastoris inoculant

[0083] The composition of the liquid fermentation medium is by weight (w / w): glucose 3.5%, soybean meal 2.0%, ammonium chloride 0.26%, magnesium sulfate 0.03%, potassium dihydrogen phosphate 0.1%, dipotassium hydrogen phosphate 0.03%, sulfuric acid Manganese 0.01%, pH6.2;

[0084] Liquid fermentation...

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PUM

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Abstract

The invention belongs to the technical field of black and odorous water body treatment, and provides a composite microbial remediation agent for a black and odorous water body and an application thereof. The compound microbial remediation agent for the black and odorous water body is composed of bacillus subtilis, pichia pastoris, candida tropicalis and a nitrifying bacterial agent. The weight ratio of the four bacterial agents is bacillus subtilis:pichia pastoris:candida tropicalis:nitrifying bacterial agent=(3-5):(1-3):(1-2):(1-2). The bacillus subtilis has a specific and efficient degradation effect on organic matters of black and odorous water and is compounded with specific bacterial agents, and the compound microbial remediation agent for the black and odorous water body is obtained.The compound microbial remediation agent is applied to treatment of the black and odorous water body, and has the advantages of all the bacterial agents, and degrades the black and odorous water bodyto the maximum extent. With adopting of the compound microbial remediation agent, after treatment, the odor of the water body is eliminated, the water body is clarified and transparent, and the waterquality COD and the ammonia nitrogen contents meet the V-class standard of 'environmental quality standards for surface water'.

Description

technical field [0001] The invention relates to the technical field of treatment of black and odorous water bodies, in particular to a composite bacterial agent for microbial restoration of black and odorous water bodies and its application. Background technique [0002] With the rapid development of economy, the continuous acceleration of urbanization process, and the sharp increase of urban population, urban rivers have been seriously polluted, black and odorous water bodies have appeared in large areas, and organic pollutants in water bodies such as sugars, amino acids, proteins, oils, etc. Decompose into small molecular substances, consume a large amount of dissolved oxygen; inorganic pollutants such as FeS and MnS, which are easily oxidized, cause the water to be black; the concentration of ammonia nitrogen and sulfide increases, causing odor, which seriously affects the image of the city and public life. [0003] Black and odorous water bodies in urban areas not only ...

Claims

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Application Information

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IPC IPC(8): C12N1/20C12N1/16C02F3/34C12R1/125C12R1/84C12R1/74
CPCC12N1/16C12N1/20C02F3/34C02F3/347C02F2303/02C02F2103/007
Inventor 潘冬梅马韵升杨传伦车树刚傅英旬魏征韩立霞倪建龙马娜娜杨丹丹
Owner CHAMBROAD CHEM IND RES INST CO LTD
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